Search results for "domain"
showing 10 items of 2485 documents
Membrane topology and post-translational modification of the Saccharomyces cerevisiae essential protein Rot1.
2007
ROT1 is an essential gene that has been related to cell wall biosynthesis, the actin cytoskeleton and protein folding. In order to help to understand its molecular function, we carried out a characterization of the Rot1 protein. It is primarily located at the endoplasmic reticulum-nuclear membrane facing the lumen. Rot1 migrates more slowly than expected, which might suggest post-translational modification. Our results indicate that Rot1 is a protein that is neither GPI-anchored nor O-glycosylated. In contrast, it is N-glycosylated. By a directed mutagenesis of several Asn residues, we identified that the protein is simultaneously glycosylated at N103, N107 and N139. Although the mutation o…
Nonlinear vibrations of elastic beams with external general visco-elastic devices
2011
Vibration damping for slender beams is achieved by applying devices at external points. The latter consist of general single visco-elastic springpot elements. An approximate nonlinear boundary value problem is found in frequency domain that holds for moderately large vibrations or for linear beams with external nonlinear devices, both in the vicinity of primary resonances. The interaction force of a so-called springpot element is expressed as a sum of two separate forces: the first develops due to the external loading function at the device location, and the second contribution arises due to an imposed time-harmonic support excitation with no other external forces acting on the structure. F…
A Viscosity Equation for Minimizers of a Class of Very Degenerate Elliptic Functionals
2013
We consider the functional $$J(v) = \int_\varOmega\bigl[f\bigl(|\nabla v|\bigr) - v\bigr] dx, $$ where Ω is a bounded domain and f:[0,+∞)→ℝ is a convex function vanishing for s∈[0,σ], with σ>0. We prove that a minimizer u of J satisfies an equation of the form $$\min\bigl(F\bigl(\nabla u, D^2 u\bigr), |\nabla u|-\sigma\bigr)=0 $$ in the viscosity sense.
Attention Direction in Static and Animated Diagrams
2010
Two key requirements for comprehending a diagram are to parse it into appropriate components and to establish relevant relationships between those components. These requirements can be particularly demanding when the diagram is complex and the viewers are novices in the depicted domain. Lack of domain-specific knowledge for top-down guidance of visual attention prejudices novices' extraction of task-relevant information. Static diagrams designed for novices often include visual cues intended to improve such information extraction. However, because current approaches to cueing tend to be largely intuitive, their effectiveness can be questionable. Further, animated diagrams with their percept…
Controlled assembly of single colloidal crystals using electro-osmotic micro-pumps.
2017
We assemble charged colloidal spheres at deliberately chosen locations on a charged unstructured glass substrate utilizing ion exchange based electro-osmotic micro-pumps. Using microscopy, a simple scaling theory and Brownian Dynamics simulations, we systematically explore the control parameters of crystal assembly and the mechanisms through which they depend on the experimental boundary conditions. We demonstrate that crystal quality depends crucially on the assembly distance of the colloids. This is understood as resulting from the competition between inward transport by the electro-osmotic pump flow and the electro-phoretic outward motion of the colloids. Optimized conditions include sub…
Modelling the Water Retention Domain of a Compacted Scaly Clay
2012
A body of experimental data on the water retention behaviour of compacted samples of an Italian scaly clay is analysed. The mechanical and the hydraulic behaviour of compacted samples of this clay are governed by multi-scale arrangement of scales and aggregates, which characterises the compacted clay fabric. At least two different pore networks, namely intra-aggregate and inter-aggregate, may be identified, which interact one with the other along coupled hydro-mechanical paths. The retention behaviour is interpreted in the framework of a comprehensive multi-scale modelling approach recently proposed for compacted clays of low and medium activity, which proves to be able to account for the i…
Low levels of WWOX protein immunoexpression correlate with tumour grade and a less favourable outcome in patients with urinary bladder tumours
2008
Aims: To correlate the immunohistochemical detection of WWOX with histological measures and disease progression within the whole spectrum of urothelial bladder neoplasms. Methods and results: One hundred and one patients with primary bladder tumours were retrospectively analysed. Immunohistochemically, a polyclonal antibody was utilized and the level of WWOX protein expression was analysed by using a combined score system based on intensity of the reaction and percentage of immunoreactive tumour cells. WWOX protein expression was consistently expressed in non-neoplastic urothelium, whereas a progressive loss of immunoreactivity was observed as tumour grade and stage increased (P < 0.05). …
WWOX-related encephalopathies: delineation of the phenotypical spectrum and emerging genotype-phenotype correlation
2014
International audience; BACKGROUND:Homozygous mutations in WWOX were reported in eight individuals of two families with autosomal recessive spinocerebellar ataxia type 12 and in two siblings with infantile epileptic encephalopathy (IEE), including one who deceased prior to DNA sampling.METHODS:By combining array comparative genomic hybridisation, targeted Sanger sequencing and next generation sequencing, we identified five further patients from four families with IEE due to biallelic alterations of WWOX.RESULTS:We identified eight deleterious WWOX alleles consisting in four deletions, a four base-pair frameshifting deletion, one missense and two nonsense mutations. Genotype-phenotype correl…
Universal charts for optical difference frequency generation in the terahertz domain
2010
We present a universal and rigorous approach to study difference frequency generation in the terahertz domain, keeping the number of degrees of freedom to a minimum, through the definition of a suitable figure of merit. The proposed method relies on suitably normalized charts, that enable to predict the optical-to-terahertz conversion efficiency of any system based on wave propagation in quadratic nonlinear materials. The predictions of our approach are found to be in good agreement with the best experimental results reported to date, enabling also to estimate the d22 nonlinear coefficient of high quality GaSe.
A posteriori error estimates for Webster's equation in wave propagation
2015
We consider a generalised Webster’s equation for describing wave propagation in curved tubular structures such as variable diameter acoustic wave guides. Webster’s equation in generalised form has been rigorously derived in a previous article starting from the wave equation, and it approximates cross-sectional averages of the propagating wave. Here, the approximation error is estimated by an a posteriori technique. peerReviewed