Search results for "domain"

Rational backbone redesign of a fructosyl peptide oxidase to widen its active site access tunnel

2020

Fructosyl peptide oxidases (FPOXs) are enzymes currently used in enzymatic assays to measure the concentration of glycated hemoglobin and albumin in blood samples, which serve as biomarkers of diabetes. However, since FPOX are unable to work directly on glycated proteins, current enzymatic assays are based on a preliminary proteolytic digestion of the target proteins. Herein, to improve the speed and costs of the enzymatic assays for diabetes testing, we applied a rational design approach to engineer a novel enzyme with a wider access tunnel to the catalytic site, using a combination of Rosetta design and molecular dynamics simulations. Our final design, L3_35A, shows a significantly wider …

Structures, interactions and packing of filamin domains

2011

Identity Processes in Adulthood: Diverging Domains

2005

Patterns of identity formation were analyzed in a longitudinal framework, from ages 27 to 36 and then to 42 years of age. Information from all 3 ages was available for 197 participants (100 women, 97 men). A variation of Marcia’s (1966) Identity Status Interview included 5 domains: religious beliefs, political identity, occupational career, intimate relationships, and lifestyle. Great variability in identity status assessments was found across the domains at each age level. The domains representing work and family (occupation, relationships, and lifestyle) were more salient for middle- aged adults than were ideological domains (religion and politics). Development along the hypothesized sequ…

A Self-powered Ambient Light Power Measurement Platform with Time-domain Readout

2021

Internet of Things (IoT) provides more and more adequate infrastructures for many applications and advanced services. This scenario promotes the ability to satisfy the growing demand for innovative measuring systems, increasingly popular consisting of intelligent nodes, totally wireless, easy to install, and that requires the least possible maintenance. In this context, this paper aims to contribute to state of the art with an energy-autonomous and battery-free measurement platform designed to measure the ambient light power when its level exceeds the sensitivity threshold. The measurement platform utilizes a single transducer to scavenge energy and concurrently measure the ambient light in…

COMMENT OPTIMISER LA DYNAMIQUE RELATIONNELLE PATIENT-PHARMACIEN POUR ASSURER UNE ALLIANCE THÉRAPEUTIQUE

2010

Cette thèse étudie la dynamique relationnelle à l'officine entre un pharmacien et un patient, et donne certaines clés pour l'améliorer. Le pharmacien, en comprenant mieux son patient, pourra créer et maintenir une véritable alliance thérapeutique avec lui, dans le but d'optimiser sa prise en charge. Ainsi, le patient deviendra un acteur de son système de soins.

Hsp60, a Novel Target for Antitumor Therapy: Structure-Function Features and Prospective Drugs Design

2013

Heat shock protein 60 kDa (Hsp60) is a chaperone classically believed to be involved in assisting the correct folding of other mitochondrial proteins. Hsp60 also plays a role in cytoprotection against cell stressors, displaying for example, antiapoptotic potential. Despite the plethora of studies devoted to the mechanism of Hsp60's function, especially in prokaryotes, fundamental issues still remain unexplored, including the definition of its role in cancer. Key questions still unanswered pertain to the differences in structure-function features that might exist between the well-studied prokaryotic GroEL and the largely unexplored eukaryotic Hsp60 proteins. In this article we discuss these …

Spatially restricted expression of PlOtp, a Paracentrotus lividus Orthopedia-related homeobox gene, is correlated with oral ectodermal patterning and…

1999

ABSTRACT Several homeobox genes are expressed in the sea urchin embryo but their roles in development have yet to be elucidated. Of particular interest are homologues of homeobox genes that in mouse and Drosophila are involved in patterning the developing central nervous system (CNS). Here, we report the cloning of an orthopedia (Otp)-related gene from Paracentrotus lividus, PlOtp. Otp is a single copy zygotic gene that presents a unique and highly restricted expression pattern. Transcripts were first detected at the mid-gastrula stage in two pairs of oral ectoderm cells located in a ventrolateral position, overlying primary mesenchyme cell (PMC) clusters. Increases in both transcript abund…

Molecular markers for identified neuroblasts in the developing brain of Drosophila.

2003

The Drosophila brain develops from the procephalic neurogenic region of the ectoderm. About 100 neural precursor cells (neuroblasts) delaminate from this region on either side in a reproducible spatiotemporal pattern. We provide neuroblast maps from different stages of the early embryo (stages 9, 10 and 11, when the entire population of neuroblasts has formed), in which about 40 molecular markers representing the expression patterns of 34 different genes are linked to individual neuroblasts. In particular, we present a detailed description of the spatiotemporal patterns of expression in the procephalic neuroectoderm and in the neuroblast layer of the gap genes empty spiracles, hunchback, hu…

Number, identity, and sequence of the Drosophila head segments as revealed by neural elements and their deletion patterns in mutants.

1994

The development of the insect head tagma involves massive rearrangements and secondary fusions of segment anlagen during embryogenesis. Due to the lack of reliable morphological markers, the number, identity, and sequence of the head segments, particularly in the pregnathal region, are still a matter of ongoing debates. We examined the complex array of internal structures of the embryonic Drosophila melanogaster head such as the sensory structures and nerves of the peripheral and stomatogastric nervous systems, and we used embryonic head mutations causing a lack of overlapping segment anlagen to unravel the segmental identity and the sequence of the neural elements. Our results provide evid…

Regulatory sequences driving expression of the sea urchin Otp homeobox gene in oral ectoderm cells.

2005

Abstract PlOtp (Orthopedia), a homeodomain-containing transcription factor, has been recently characterized as a key regulator of the morphogenesis of the skeletal system in the embryo of the sea urchin Paracentrotus lividus . Otp acts as a positive regulator in a subset of oral ectodermal cells which transmit short-range signals to the underlying primary mesenchyme cells where skeletal synthesis is initiated. To shed some light on the molecular mechanisms involved in such a process, we begun a functional analysis of the cis -regulatory sequences of the Otp gene. Congruent with the spatial expression profile of the endogenous Otp gene, we found that while a DNA region from −494 to +358 is s…