Search results for "echinostoma"

Helminth-Trematode: Echinostoma

2014

The term, echinostomes, includes those digeneans belonging to the family Echinostomatidae. Echinostomes are a rather heterogeneous group of cosmopolitan hermaphroditic digeneans that inhabit, as adults, the intestine of a great spectrum of vertebrate hosts, such as birds, mammals and, occasionally, reptiles and fishes. They can also parasitize humans causing the food-borne infection called echinostomiasis. The definitive hosts become infected after ingestion of the second intermediate host harboring the encysted metacercariae. Clinical symptoms of echinostomiasis include abdominal pain, violent watery diarrhea, and anorexia. The disease occurs focally and transmission is linked to fresh or …

Criteria for species determination in the 'revolutum' group of Echinostoma.

2004

Expresión diferencial de proteínas en el íleon de ratón asociadas con el desarrollo de infecciones crónicas por Echinostoma caproni

2015

Entre las enfermedades parasitarias que afectan a la humanidad, las helmintiasis intestinales destacan por su gran morbilidad, ya que actualmente afectan a más de 2000 millones de personas y causan grandes pérdidas económicas relacionadas con la ganadería y la veterinaria. Echinostoma caproni es un trematodo intestinal que, por sus características, es utilizado en numerosos estudios como modelo experimental para determinar los factores por los que se resuelve una infección aguda o se desarrolla una infección crónica causada por helmintos intestinales. En este trabajo se analizó la expresión diferencial de proteínas durante una primoinfección, después de un tratamiento farmacológico y durant…

Differential expression and glycosylation of proteins in the rat ileal epithelium in response to Echinostoma caproni infection.

2013

Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode that has been used as experimental model to investigate the factors determining the expulsion of intestinal helminths. We analyze the changes in the protein expression and glycosylation induced by E. caproni in Wistar rat, a host of low compatibility in which the parasites are rapidly rejected. To determine the changes in protein expression, two-dimensional difference gel electrophoresis was employed using protein extracts from the intestine of naïve and infected rats. The patterns of glycosylation were analyzed by lectin blotting. Those spots showing differential expression or glycosylation were analyzed by mass s…

Echinostomes in the definitive host: a model for the study of host-parasite relationships.

2008

Echinostomes are hermaphroditic digeneans that parasitize, as adults, numerous vertebrate hosts of all classes. In this chapter, the main features of the development of adult echinostome in the definitive host are analyzed in detail. The stage of the echinostome in the definitive host undergoes a number of features such as metacercarial excystment, establishment, habitat location, adult development, mate, and release of eggs. These features are documented with an emphasis in their potential use as experimental models. The analysis is focused on members of the genus Echinostoma, although members of other genera of the Echinostomatidae are mentioned when they are relevant to the discussion. A…

Specific tyrosine phosphorylation in response to bile in Fasciola hepatica and Echinostoma friedi

2003

Protein tyrosine phosphorylation (PY) is a well-known signalling mechanism which is also involved in host-parasite interactions. Despite its transcendence, PY has been poorly studied in parasitic helminths. The aim of this study is to examine the effect of bile salts on the PY pattern in parasitic trematodes. Two distinct adult models were analysed: Echinostoma friedi, of intestinal habitat, and Fasciola hepatica, naturally inhabitant of host biliary channels. Our results show that bile salts induce specific and distinct protein PY in both trematode species, indicating that this signalling process seems to be also involved in host-trematode relationships.

Survival and infectivity of Hypoderaeum conoideum and Euparyphium albuferensis cercariae under laboratory conditions.

1999

The survival characteristics of the cercariae of Hypoderaeumconoideum and Euparyphiumalbuferensis (Trematoda: Echinostomatidae) at 20°C and 30°C are described, and the age dependency of their infectivity at 20°C is studied to determine their respective transmission efficiencies. Cercarial survival was found to be age-dependent and was higher at 20°C. For both cercariae, the maximum life-span was 26 h at 20°C and 16 h at 30°C, and their respective times to 50% mortality were similar at each temperature. Both cercariae seem to be well adapted to transmission in their natural habitat, though cercarial infectivity of H. conoideum was higher than that of E. albuferensis, this being correlated wi…

Echinostoma friedi: the effect of age of adult worms on the infectivity of miracidia.

2004

AbstractThe effect of ageing of adults ofEchinostoma friedi(Trematoda: Echinostomatidae) on the infectivity of miracidia yielded was analysed. Miracidia were obtained after hatching of eggs obtained from adult worms ofE. friedicollected weekly during the course of experimental infections in golden hamsters. Miracidial infectivity, measured in terms of percentage of infection inLymnaea peregra, was significantly influenced by the age of the adult worms from which the miracidia were derived. Infective miracidia only were obtained from adult worms in the age range from 4 to 9 weeks post-infection. Infectivity was maximal in those miracidia derived from adults collected 8 and 9 weeks post-infec…

Effects of Nonschistosome Larval Trematodes on Biomphalaria Snails

2010

This chapter examines the most salient literature on the development of nonschistosome larval digeneans in Biomphalaria spp. (members of the ­superfamilies Clinostomoidea, Diplostomoidea, Echinostomatoidea, Paramphistomoidea, and Pronocephaloidea). For this purpose, the general biology of the nonschistosome trematode species that use Biomphalaria spp. as ­intermediate hosts are ­analyzed. Thereafter, the main features of these parasitic infections are studied with ­particular emphasis on topics such as the effect of the infections on the snail, the antagonism and/or competition between different digenean species in the snail, the ­immunology of the infection, and the genomic and proteomic a…

Development of an antibody-based capture enzyme-linked immunosorbent assay for detecting echinostoma caproni (trematoda) in experimentally infected r…

2003

The present study reports on the development of a coproantigen capture enzyme-linked immunosorbent assay (ELISA) for detecting Echinostoma caproni in experimentally infected rats. The capture ELISA was based on polyclonal rabbit antibodies that recognize excretory–secretory (ES) antigens. The detection limit of pure ES was 3 ng/ml in sample buffer and 60 ng/ml in fecal samples. The test was evaluated using a follow-up of 10 rats experimentally infected with 100 metacercariae of E. caproni, and the results were compared with those of other diagnostic methods such as parasitological examination and antibody titers determined by indirect ELISA. Coproantigens were detected in all the infected r…