Search results for "echovirus"
showing 10 items of 35 documents
Infectious Entry Pathway of Enterovirus B Species
2015
Enterovirus B species (EV-B) are responsible for a vast number of mild and serious acute infections. They are also suspected of remaining in the body, where they cause persistent infections contributing to chronic diseases such as type I diabetes. Recent studies of the infectious entry pathway of these viruses revealed remarkable similarities, including non-clathrin entry of large endosomes originating from the plasma membrane invaginations. Many cellular factors regulating the efficient entry have recently been associated with macropinocytic uptake, such as Rac1, serine/threonine p21-activated kinase (Pak1), actin, Na/H exchanger, phospholipace C (PLC) and protein kinase Cα (PKCα). Another…
Enterovirus-induced non-acidic entry pathway and its relation to the epidermal growth factor receptor pathway
2014
Infectious Entry Pathway of Enterovirus B Species
2015
ESCRT-kompleksien merkitys echovirus 1 -infektiossa ja kaveosomin monivesikkelisen rakenteen muodostumisessa
2008
Endosomin sisäisen ioniympäristön vaikutukset echovirus 1 -infektioon
2009
Cholesterol Dependence of Collagen and Echovirus 1 Trafficking along the Novel alpha2beta1 Integrin Internalization Pathway
2013
We have previously shown that soluble collagen and a human pathogen, echovirus 1 (EV1) cluster α2β1 integrin on the plasma membrane and cause their internalization into cytoplasmic endosomes. Here we show that cholesterol plays a major role not only in the uptake of α2β1 integrin and its ligands but also in the formation of α2 integrin-specific multivesicular bodies (α2-MVBs) and virus infection. EV1 infection and α2β1 integrin internalization were totally halted by low amounts of the cholesterol-aggregating drugs filipin or nystatin. Inhibition of cholesterol synthesis and accumulation of lanosterol after ketoconazole treatment inhibited uptake of collagen, virus and clustered integrin, an…
Integrin-mediated entry of echovirus 1
2008
Paula Upla selvitti väitöskirjassaan echovirus 1:n solunsisäistä reittiä ja tunnisti solurakenteita ja säätelijöitä, jotka ovat tärkeitä infektiossa. Echovirus 1 (EV1) kuuluu pikornavirusperheeseen, jonka muita tunnettuja edustajia ovat poliovirus sekä karjan suu- ja sorkkatautivirus. EV1 on pieni (25-30 nm), vaipaton RNA-virus. EV1 aiheuttaa useimmiten oireettomia tai vähäoireisia suolisto- ja hengitystieinfektioita, mutta myös vakavampia tiloja, kuten aivokalvontulehdusta.Upla seurasi viruksen tunkeutumista soluun käyttäen elektronimikroskopiaa sekä tehokasta kolmi- ja neliulotteista konfokaalimikroskopiaa. Näin hän pystyi havainnoimaan yhtä aikaa usean eri molekyylin vuorovaikutusta niih…
Echovirus 1 entry into polarized Caco-2 cells depends on dynamin, cholesterol, and cellular factors associated with macropinocytosis
2013
Enteroviruses invade their hosts by crossing the intestinal epithelium. We have examined the mechanism by which echovirus 1 (EV1) enters polarized intestinal epithelial cells (Caco-2). Virus binds to VLA-2 on the apical cell surface and moves rapidly to early endosomes. Using inhibitory drugs, dominant negative mutants, and small interfering RNAs (siRNAs) to block specific endocytic pathways, we found that virus entry requires dynamin GTPase and membrane cholesterol but is independent of both clathrin- and caveolin-mediated endocytosis. Instead, infection requires factors commonly associated with macropinocytosis, including amiloride-sensitive Na /H exchange, protein kinase C, and C-termina…