Search results for "erk"
showing 10 items of 2599 documents
Chironomid incorporation of methane-derived carbon in plankton- and macrophyte-dominated habitats in a large shallow lake
2018
1. While 13 C-depleted carbon derived from biogenic methane can substantially contribute to the benthic secondary production in deep stratified lakes, its role in shallow lakes is less clear. We investigated the dynamics of δ13 C and δ15 N in the larvae of Chironomus plumosus throughout an annual cycle in two ecologically distinct basins (open-water plankton-dominated and sheltered macrophyte- covered) of a large (270 km2 ), shallow, polymictic and eutrophic lake (Võrtsjärv, Estonia, North Europe). The larval stable isotopic compositions were linked to the presence of methane-oxidising bacteria (MOB) in larval guts and sediments. 2. Molecular detection of MOB revealed their presence in vari…
Species interactions, environmental gradients and body size shape population niche width
2021
1. Competition for shared resources is commonly assumed to restrict population-level niche width of coexisting species. However, the identity and abundance of coexisting species, the prevailing environmental conditions, and the individual body size may shape the effects of interspecific interactions on species’ niche width. 2. Here we study the effects of interspecific and intraspecific interactions, lake area and altitude, and fish body size on the trophic niche width and resource use of a generalist predator, the littoral-dwelling large, sparsely rakered morph of European whitefish (Coregonus lavaretus; hereafter LSR whitefish). We use stable isotope, diet and survey fishing data from 14 …
Whole-Lake Sugar Addition Demonstrates Trophic Transfer of Dissolved Organic Carbon to Top Consumers
2017
Terrestrial dissolved organic carbon (DOC) provides an external carbon source to lake ecosystems. However, there is ongoing debate about whether external DOC that enters a lake can pass up the food web to support top consumers. We show, from experimental manipulation of a whole lake, that externally loaded DOC can contribute appreciably to fish biomass. Monthly additions of cane sugar with a distinct carbon stable isotope value during 2 years rapidly enriched the 13C content of zooplankton and macroinvertebrates, with a more gradual 13C enrichment of fish. After sugar addition stopped, the 13C content of consumers reverted towards original values. A simple isotope mixing model indicated tha…
Peer-to-peer cooperative GNSS-based localization for stationary reference nodes in wireless sensor networks
2017
Most localization algorithms in wireless sensor networks rely on a few reference nodes with known locations to estimate the locations of unknown nodes. The locations of reference nodes can be either manually configured or, more practically, obtained by means of some satellite-based positioning system(s). However, satellite-based locations may be inaccurate and imprecise, which results in reduced location accuracy of localization algorithms. This paper proposes a peer-to-peer cooperative GNSS-based localization algorithm for stationary reference nodes to improve their relative location accuracy and precision. The algorithm applies simple statistical methods and GNSS-based information from mu…
Using Recorded Audio Feedback in Cross-Cultural e-Education Environments to Enhance Assessment Practices in a Higher Education
2018
Providing feedback to learners on their writing assignments is perhaps one of the most important and time-consuming tasks that a supervisor performs. In e-Education environments, giving feedback becomes more challenging because there are often no possibilities for face-to-face discussions with learners. Typically, a supervisor provides comments to learners in written form via email; however, the use of recorded audio feedback (RAF) in e-Education environments has become a viable alternative. The purpose of this case study was to examine learners’ perceptions of RAF and written feedback for their assignments at the University of Jyväskylä (Finland) and at Keio University SFC (Japan). Formati…
Extreme minimal learning machine: Ridge regression with distance-based basis
2019
The extreme learning machine (ELM) and the minimal learning machine (MLM) are nonlinear and scalable machine learning techniques with a randomly generated basis. Both techniques start with a step in which a matrix of weights for the linear combination of the basis is recovered. In the MLM, the feature mapping in this step corresponds to distance calculations between the training data and a set of reference points, whereas in the ELM, a transformation using a radial or sigmoidal activation function is commonly used. Computation of the model output, for prediction or classification purposes, is straightforward with the ELM after the first step. In the original MLM, one needs to solve an addit…
Mitigation of Fatigue Damage and Vibration Severity of Electric Drivetrains by Systematic Selection of Motion Profiles
2016
The offshore drilling industry is among the most demanding markets for electrical equipment. Heave motion, irregular cyclic loads, harsh weather conditions, and vibrations are causing accelerated deterioration of drilling equipment. One of the most common solutions to these problems is to design actuation systems of such machinery overly conservative to gain additional safety, which results in too high initial investment and maintenance costs. To mitigate the fatigue damage and vibration severity of rotating elements of electric drivetrains operating offshore, this paper presents a comparative analysis of four popular input functions used in motion control of industrial systems. We evaluate…
Expression of NF-κB and IL-6 in oral precancerous and cancerous lesions: An immunohistochemical study.
2014
Background The purpose of this study was to evaluate the immunohistochemical expression of NF-κB and IL-6 in oral premalignant and malignant lesions and to investigate their possible correlation with the presence of subepithelial inflammation. Material and Methods Thirty two oral premalignant lesions, clinically compatible with leukoplakia or erythroplakia, were investigated. Microscopically, 11 of them showed hyperkeratosis and acanthosis (epithelial hyperplasia) and 21 showed dysplasia of varying degrees. Nine cases of OSCC and four control cases of normal oral mucosa were also included in the study. Immunohistochemical staining with NF-κB (p65) and IL-6 was performed. IL-6 and nuclear NF…
Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus
2017
Dysregulation of NO production is implicated in pregnancy-related diseases, including gestational diabetes mellitus (GDM). The role of NO and its placental targets in GDM pregnancies has yet to be determined. S-Nitrosylation is the NO-derived posttranslational protein modification that can modulate biological functions by forming NO-derived complexes with longer half-life, termed S-nitrosothiol (SNO). Our aim was to examine the presence of endogenous S-nitrosylated proteins in cysteine residues in relation to antioxidant defense, apoptosis, and cellular signal transduction in placental tissue from control (n=8) and GDM (n=8) pregnancies. S-Nitrosylation was measured using the biotin-switch …
Desensitization of cAMP Accumulation via Human β3-Adrenoceptors Expressed in Human Embryonic Kidney Cells by Full, Partial, and Biased Agonists
2019
β3-Adrenoceptors couple not only to cAMP formation but, at least in some cell types, also to alternative signaling pathways such as phosphorylation of extracellular signal-regulated kinase (ERK). β3-Adrenoceptor agonists are used in long-term symptomatic treatment of the overactive bladder syndrome; it is only poorly understood which signaling pathway mediates the clinical response and whether it undergoes agonist-induced desensitization. Therefore, we used human embryonic kidney cells stably transfected with human β3-adrenoceptors to compare coupling of ligands with various degrees of efficacy, including biased agonists, to cAMP formation and ERK phosphorylation, particularly regarding des…