Search results for "estradioli"
showing 10 items of 15 documents
Design and protocol of Estrogenic Regulation of Muscle Apoptosis (ERMA) study with 47 to 55-year-old women's cohort : novel results show menopause-re…
2018
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Estrogen Regulates the Satellite Cell Compartment in Females
2019
SUMMARY Skeletal muscle mass, strength, and regenerative capacity decline with age, with many measures showing a greater deterioration in females around the time estrogen levels decrease at menopause. Here, we show that estrogen deficiency severely compromises the maintenance of muscle stem cells (i.e., satellite cells) as well as impairs self-renewal and differentiation into muscle fibers. Mechanistically, by hormone replacement, use of a selective estrogen-receptor modulator (bazedoxifene), and conditional estrogen receptor knockout, we implicate 17β-estradiol and satellite cell expression of estrogen receptor α and show that estrogen signaling through this receptor is necessary to preven…
Associations of resting and peak fat oxidation with sex hormone profile and blood glucose control in middle-aged women.
2022
Background and Aims Menopause may reduce fat oxidation. We investigated whether sex hormone profile explains resting fat oxidation (RFO) or peak fat oxidation (PFO) during incremental cycling in middle-aged women. Secondarily, we studied associations of RFO and PFO with glucose regulation. Method and Results We measured RFO and PFO of 42 women (age 52–58 years) with indirect calorimetry. Seven participants were pre- or perimenopausal, 26 were postmenopausal, and nine were postmenopausal hormone therapy users. Serum estradiol (E2), follicle-stimulating hormone, progesterone, and testosterone levels were quantified with immunoassays. Insulin sensitivity (Matsuda index) and glucose tolerance (…
Ympäristöestrogeeniseoksen yhteisvaikutus 20dpfZF-seeprakalan vitellogeniinigeenin ilmentymiseen
2011
Ihmisten toimesta ympäristöön joutuvien vierasaineiden vaikutukset hormonitoimintaan ovat viime vuosina herättäneet paljon keskustelua. Yksi alan tutkituimmista aihealueista on estrogeenin kaltaisesti vaikuttavien aineiden eli ympäristöestrogeenien aiheuttamat lisääntymis- ja kehityshäiriöt. Estrogeenisiä vaikutuksia voidaan tutkia monella tapaa, alkaen solutason vasteista populaatiokehitykseen. Yksi paljon käytetty molekyylimarkkeri on kalojen vitellogeniinitason mittaus. Vaikka normaalioloissa vain naaraat tuottavat vitellogeniiniä, estrogeenialtistuksen vaikutuksesta myös koiraat ja nuoret yksilöt voivat sitä tuottaa. Tässä opinnäytetyössä tutkittiin kolmen ympäristöestrogeenin, bisfenol…
Mechanisms of honest sexual signalling and life history trade-offs in three cyprinid fishes
2005
Anssi Vainikka on väitöskirjassaan selvittänyt vaikuttavatko särjen ja suutarin ornamenttien tuottoon kytketyt hormonit niiden immuunipuolustuskykyyn. Vastoin ennakko-oletuksia, testosteronin tai estradiolin ei havaittu alentavan immuunipuolustuskykyä. Myöskään ornamenttien ja immuunipuolustuskyvyn mittareiden väliltä ei löytynyt seksuaalivalinnan teorioiden ennustamia positiivisia yhteyksiä. Korkea testosteronipitoisuus oli kuitenkin yhteydessä koirassuutarin hyvään kuntoon ja ornamentit kertovatkin koiraan hyvästä elinkyvystä. Testosterone is known to enhance reproductive success, but has also been proposed to suppress immune defence. Prior to spawning, male roach (Rutilus rutilus L.) pro…
Hormonikorvaushoito ja luurankolihaksisto : estrogeenisten ja progestogeenisten komponenttien vaikutukset lihassoluviljelmässä
2010
Yksi merkittävimmistä ikääntymiseen liittyvistä tapahtumista on luurankolihasten heikentyminen. Sarkopenia eli lihaskato heikentää ennen kaikkea ikääntyvän ihmisen terveyttä ja sitä kautta elämänlaatua, mutta sen kustannukset myös sosiaali- ja terveydenhuollolle ovat suuret. Naisilla lihasten suorituskyky heikkenee merkittävästi vaihdevuosien aikana, jolloin myös estrogeenin ja progesteronin tasot laskevat. Etenkin 17β-estradiolin (E2) tuotanto munasarjoissa loppuu vaihdevuosien aikana. Estrogeenin tiedetään vaikuttavan primaaristen sukupuoliominaisuuksien lisäksi myös moniin muihin kudoksiin, kuten luuhun ja lihaksiin. Vaihdevuosien mukanaan tuomia oireita hoidetaan usein estradiolia ja pr…
Effects of combined hormone replacement therapy or its effective agents on the IGF-1 pathway in skeletal muscle.
2010
Objectives To investigate the effects of combined hormone replacement therapy (HRT) and its effective agents on the IGF-1 signaling pathway. Design and methods To examine the effects of HRT on skeletal muscle in vivo, we utilized pre- and post-intervention samples from a randomized double blinded trial with 50–57-year-old women. The intervention included the year-long use of either HRT preparation (2 mg 17β-estradiol, E2; 1 mg norethisterone acetate, NETA, n = 10) or placebo (CO, n = 9). Microarray technology and quantitative PCR (qPCR) were used to study the expression of insulin-like growth factor I (IGF-1) and its splice variants as well as IGF-1 receptor, Akt1, mTOR, FOXO1, FOXO3, atrog…
Muscle function in monozygotic female twin pairs discordant for hormone replacement therapy
2011
Introduction: Postmenopausal monozygotic twin pairs discordant for hormone replacement therapy (HRT) provide an advantageous study design controlling for genetic background for elucidating the relationships between aging, sex hormone levels, muscle strength, contractile capacity, and fatigability. Methods: Thirteen postmenopausal monozygotic twin pairs discordant for HRT were measured for maximal voluntary torque (MVC) and twitch characteristics using electrical stimulation before and after intermittent dynamic plantarflexor exercise until exhaustion. Results: Peak twitch torque was 32% higher in HRT users than in their non-HRT, genetically identical sisters (P = 0.002), but MVC did not dif…
Menopause modulates the circulating metabolome: evidence from a prospective cohort study
2022
Abstract Aims We studied the changes in the circulating metabolome and their relation to the menopausal hormonal shift in 17β-oestradiol and follicle-stimulating hormone levels among women transitioning from perimenopause to early postmenopause. Methods and results We analysed longitudinal data from 218 Finnish women, 35 of whom started menopausal hormone therapy during the study. The menopausal transition was monitored with menstrual diaries and serum hormone measurements. The median follow-up was 14 months (interquartile range: 8–20). Serum metabolites were quantified with targeted nuclear magnetic resonance metabolomics. The model results were adjusted for age, follow-up duration, educat…
Estradiol deficiency and skeletal muscle apoptosis: Possible contribution of microRNAs.
2020
Background Menopause leads to estradiol (E2) deficiency that is associated with decreases in muscle mass and strength. Here we studied the effect of E2 deficiency on miR-signaling that targets apoptotic pathways. Methods C57BL6 mice were divided into control (normal estrous cycle, n = 8), OVX (E2 deficiency, n = 7) and OVX + E2 groups (E2-pellet, n = 4). Six weeks following the OVX surgery, mice were sacrificed and RNA isolated from gastrocnemius muscles. miR-profiles were studied with Next-generation sequencing (NGS) and candidate miRs verified using qPCR. The target proteins of the miRs were found using in silico analysis and measured at mRNA (qPCR) and protein levels (Western blot). Resu…