Search results for "extract"

Sulfated Lupane Triterpene Derivatives and a Flavone C-Glycoside from Gypsophila repens

2007

A new sulfated lupane triterpene, Gypsophilin (1), and its glucosyl ester, Gypsophilinoside (2) were isolated from the roots of Gypsophila repens whereas a new flavone C-glycoside (3) was obtained from the aerial parts. Their structures were established as (3beta)-3-O-(sulfo)lup-20(29)-en-23,28-dioic acid (1), (3beta)-3-O-(sulfo)lup-20(29)-en-23,28-dioic acid -28-O-beta-D-glucopyranosyl ester (2) and luteolin-7-O-alpha-L-arabinopyranosyl-6-C-beta-glucopyranoside (3) by spectroscopic methods such as 1D and 2D NMR, HR-ESI-MS and FAB-MS.

ImageRover: A Content-Based Image Browser for the World Wide Web

1997

ImageRover is a search-by-image-content navigation tool for the World Wide Web (WWW). To gather images expediently, the image collection subsystem utilizes a distributed fleet of WWW robots running on different computers. The image robots gather information about the images they find, computing the appropriate image decompositions and indices, and store this extracted information in vector form for searches based on image content. At search time, users can iteratively guide the search through the selection of relevant examples. Search performance is made efficient through the use of an approximate, optimized k-d tree algorithm. The system employs a novel relevance feedback algorithm that se…

Measurement of T Cell Activation After 16‐hr In Vitro Stimulation with Concanavalin A

2010

A flow cytometry assay that can be used to directly determine the proportion of activated T lymphocytes in human whole blood samples after stimulation with concanavalin A is presented here. Human whole blood is incubated with fluorescently labeled antibodies (against CD3, CD4, CD8, and CD69), erythrocytes are then lysed, and the samples are analyzed using a flow cytometer. The assay presented is able to differentiate between CD4+ and CD8+ T lymphocytes. Thus, it is possible to quantify both lymphocyte populations in parallel, as well as the respective proportions of activated T lymphocytes, all from one sample. An additional advantage of this assay is that it was developed to assay whole bl…

Sojas pupiņu un smiltsērkšķu ekstraktu saturoša matu kopšanas līdzekļa izstrāde un efektivitātes noteikšana

2018

Sojas pupiņu un smiltsērkšķa ekstraktu saturoša matu kopšanas līdzekļa izstrāde un efektivitātes noteikšana. Ostrovska G., zinātniskā vadītāja vadošā pētniece Dr. chem. Orola L. Maģistra darbs, 51 lappuses, 16 attēli, 6 tabulas, 59 literatūras avoti, 3 pielikumi. Latviešu valodā. Darbā veikta matu kopšanas līdzekļa- matu maskas bāzes izstrāde, kura papildināta ar sojas un smiltsērkšķa ekstraktiem. Sojas ekstrakts ekstrahēts no sojas pupiņam un ar AEŠH/MS metodi, noteikts ekstraktā esošo aminoskābju saturs. Novērtēta matu maskas bāzes un matu masku efektivitāte uz matu virsmas morfoloģiskajām izmaiņām, matu mehāniskajām un mitruma sorbcijas īpašībām.

ANTIPROLIFERATIVE ACTIVITY OF CYSTOSEIRA FOENICULACEA SFE EXTRACT ON HEPG2 CELL LINE.

2008

The flesh ethanolic extract of Hylocereus polyrhizus exerts anti-inflammatory effects and prevents murine colitis

2015

IBD is a chronic disorder of the gastrointestinal tract characterized by mucosal inflammation and epithelial damage. Biologic therapy has significantly improved the course of the disease but there are still a high percentage of patients that do not respond to current therapies. We aim to determine the effects of the flesh ethanolic extract of Hylocereus polyrhizus (EH) in a mice model of colitis induced by TNBS.Balb/c mice received TNBS (175 mg/kg, 100 μl, i.r.) and six and thirty hours later were administered with EH (1 g/kg, i.p.). Mice were weighted daily and after sacrificing (2 and 4 days after TNBS) we analyzed mucosal histology, myeloperoxidase activity (MPO), the expression of pro-i…

Simultaneous determination of eight underivatised biogenic amines in fish by solid phase extraction and liquid chromatography-tandem mass spectrometr…

2010

Biogenic amines on fish tissue are formed as a result of bacterial contamination and spoilage during storage. A new method based on liquid chromatography (LC) and tandem mass spectrometry (MS/MS) using a triple quadrupole (QqQ) analyser was developed for the analysis of eight biogenic amines (cadaverine, histamine, phenylethylamine, putrescine, spermine, spermidine, tyramine and tryptamine) in fish tissues. Sample preparation was performed by extraction with trichloroacetic acid 5% and solid phase extraction clean up with STRATA X cartridge. The MS/MS method was validated and compared with a method based on the analysis of dansyl derivatives by LC and fluorescence detector (FD). MS/MS achie…

Ultrasound-assisted sequential extraction method for the evaluation of mobility of toxic elements in contaminated soils

2005

A method for the fast sequential extraction of toxic elements in contaminated soil samples using an ultrasonic water bath, followed by determination with inductively coupled plasma optical emission spectrometry (ICP-OES), was developed and compared with other methods introduced in the literature. The five-step sequential extraction (Tessier scheme) was shortened using ultrasound-assisted sequential extraction (UASE). The optimization of the five-step sequential extraction was based on the analysis of SRM 2710 using Tessiers’s method as a reference. Several extracting solutions with different sonication times and temperatures were tested in the optimization procedure. Concentrations of arsen…

A new simple approach to evaluate pedogenic clay transformation in a Vertic Calcisol

2006

The aim of this study is to characterize the pedogenic clay minerals by using simple approach: Mixing mineralogical and geochemical findings. The fine clay fractions (< 0.1 μm) of a Vertic Cambisol profile were studied by means of X-ray diffraction (XRD), infrared spectroscopy (FTIR) and cation exchange capacity (CEC). Qualitative and quantitative mineralogical compositions of the clay mixture were determined. Moreover, chemical equilibria and thermodynamic stabilities of minerals (calcite, gypsum, kaolinite, smectites and illites) were studied using results of ionic activities obtained from total concentration of various aqueous species in water extracts from soil-saturated pastes. XRD ana…

In-situ suspended aggregate microextraction: A sample preparation approach for the enrichment of organic compounds in aqueous solutions.

2015

Abstract This work presents in-situ suspended aggregate microextraction (iSAME) as a new and expedient sample preparation method. This new concept capitalizes on the general principles of in-situ solvent formation microextraction, in the sense that extraction is carried out in a supramolecular aggregate phase, which is formed in-situ in the sample through one-step process involving ion-association between a cationic surfactant and a benzene sulfonic acid derivative. The suspended aggregate containing the analytes is then collected in the form of a thin-film on the surface of a common filter paper by suction filtration. The entrapped analytes are released by completely dissolving the thin-fi…