Search results for "flu"

showing 10 items of 16583 documents

Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
researchProduct

Effect of Nanoaquacitrates on Physiological Parameters of Fodder Galega Infected with Phytoplasma

2018

AbstractThe laboratory experiments have been found that soaking seedsGalega orientalisL. (Fodder galega) in nanoaquacitrates solutions of Mn (10 and 20 mg/dm3), Mo (4 mg/dm3) and Mg (2 and 4 mg/dm3), has been lead to germination energy rise, while Mn (10 and 20 mg/dm3) and Mo (4 mg/dm3) concentrations has been influenced germinating ability. At the same time, the soaking seeds in solution of nanoaquacitrates Mn (20 mg/dm3) had the biggest stimulatory effect on the accumulation 7 daily sprouts mass (on 18%). It has been shown that soaking seeds in nanoparticles Mn and Mo solutions leads to the increase of catalase activities (especially under the influence of manganese) and peroxidase activi…

0106 biological sciences0301 basic medicineVeterinary medicineEnvironmental EngineeringbiologyChemistrybiology.organism_classification01 natural sciencesGalega03 medical and health sciences030104 developmental biologyFodderPhytoplasmaAcholeplasma laidlawiiFluorescence inductionEnvironmental Chemistry010606 plant biology & botanyEcological Chemistry and Engineering S
researchProduct

Clone-Dependent Expression of Esca Disease Revealed by Leaf Metabolite Analysis

2019

International audience; Grapevine trutk diseases, especially Esca, are of major concern since they gradually alter vineyards worldwide and cause heavy economic losses. The expression of Esca disease symptoms depends on several factors, including the grapevine cultivar. In this context, a possible clone-dependent expression of the Esca disease was studied. Two clones of 'Chardonnay' grown in the same plot were compared according to their developmental and physiological traits, metabolome, and foliar symptom expression. Analysis of their leaf metabolome highlighted differences related to symptom expression. Interestingly, the content of a few specific metabolites exhibited opposite variations…

0106 biological sciences0301 basic medicine[SDV]Life Sciences [q-bio]EscaClone (cell biology)Context (language use)DiseasePlant ScienceBiologylcsh:Plant culture01 natural sciences03 medical and health sciencesMetabolomics[SDV.IDA]Life Sciences [q-bio]/Food engineeringMetabolomelcsh:SB1-1110Cultivargrapevine trunk diseasesComputingMilieux_MISCELLANEOUSOriginal Research2. Zero hungerclonefood and beveragesMetabolite analysismetabolomicsVitis vinifera;grapevine trunk diseases;clone;metabolomics;Esca;3D fluorescenceHorticulture030104 developmental biologyVitis viniferaShoot3D fluorescence[SDV.IB]Life Sciences [q-bio]/Bioengineering010606 plant biology & botany
researchProduct

2020

Turtles, a speciose group consisting of more than 300 species, demonstrate karyotypes with diploid chromosome numbers ranging from 2n = 26 to 2n = 68. However, cytogenetic analyses have been conducted only to 1/3rd of the turtle species, often limited to conventional staining methods. In order to expand our knowledge of the karyotype evolution in turtles, we examined the topology of the (TTAGGG)n telomeric repeats and the rDNA loci by fluorescence in situ hybridization (FISH) on the karyotypes of two emydids: the Sicilian pond turtle, Emys trinacris, and the yellow-bellied slider, Trachemys scripta scripta (family Emydidae). Furthermore, AT-rich and GC-rich chromosome regions were detected …

0106 biological sciences0301 basic medicinebiologymedicine.diagnostic_testZoologyKaryotypeEmydidaebiology.organism_classification010603 evolutionary biology01 natural scienceslaw.invention03 medical and health sciences030104 developmental biologyEmys trinacrislawChromosome regionsGeneticsmedicineMicrochromosomePloidyTurtle (robot)Genetics (clinical)Fluorescence in situ hybridizationGenes
researchProduct

2014

The Vipp1 protein is essential in cyanobacteria and chloroplasts for the maintenance of photosynthetic function and thylakoid membrane architecture. To investigate its mode of action we generated strains of the cyanobacteria Synechocystis sp. PCC6803 and Synechococcus sp. PCC7942 in which Vipp1 was tagged with green fluorescent protein at the C-terminus and expressed from the native chromosomal locus. There was little perturbation of function. Live-cell fluorescence imaging shows dramatic relocalisation of Vipp1 under high light. Under low light, Vipp1 is predominantly dispersed in the cytoplasm with occasional concentrations at the outer periphery of the thylakoid membranes. High light ind…

0106 biological sciences0303 health sciencesPhotosystem IIBiology01 natural sciencesMicrobiologyGreen fluorescent proteinMicrobiologyChloroplast03 medical and health sciencesMembraneCytoplasmThylakoidBiophysicsProtein biosynthesisPhage shockMolecular Biology030304 developmental biology010606 plant biology & botanyMolecular Microbiology
researchProduct

Environmental and ontogenetic constraints on developmental stability in the spatangoid sea urchin Echinocardium (Echinoidea)

2006

Biogeosciences, UMR-CNRS 5561, Universite de Bourgogne, 6 bd Gabriel, 21000 Dijon, FranceReceived 25 June 2004; accepted for publication 25 July 2005Spatangoid irregular sea urchins are detritivorous benthic organisms particularly prone to variations of environ-ment, and their mode of growth and plate morphology make them an appropriate model to assess the effects of envi-ronmental variations. Two populations of Echinocardium flavescens were sampled in two sites of the Norwegiancoast characterized by contrasted environmental conditions. Different morphological descriptors (plate areas, inter-landmarks distances, overall size, and shape of the posterior ambulacra) were used to appraise interi…

0106 biological sciences0303 health scienceseducation.field_of_studyEcologyOntogenyPopulationBiologybiology.organism_classification010603 evolutionary biology01 natural sciencesStability (probability)EchinocardiumFluctuating asymmetry03 medical and health sciencesBenthic zonebiology.animal14. Life underwaterBiogeoscienceseducationSea urchinEcology Evolution Behavior and Systematics030304 developmental biologyBiological Journal of the Linnean Society
researchProduct

Functional reconstitution of a proton-translocating system responsive to fusicoccin

1988

Crude fusicoccin binding proteins and a partially purified plasma membrane H+-transporting ATPase (EC 3.6.1.34), both solubilized from maize tissues, were simultaneously inserted into liposomes by the freeze-thaw method. ATP-driven intravesicular acidification in the proteoliposomes, measured by the fluorescence quenching of the dye 9-amino-6-chloro-2-methoxyacridine, markedly increased upon addition of fusicoccin to the reconstituted system. This effect could not be observed when binding sites and ATPase preparations were separately reconstituted into the proteoliposomes, thus demonstrating that fusicoccin binding to its receptor is a prerequisite for ATPase stimulation.

0106 biological sciencesATPase[SDV]Life Sciences [q-bio]01 natural sciences03 medical and health scienceschemistry.chemical_compoundProton transportGlycosidesBinding siteComputingMilieux_MISCELLANEOUSFluorescent Dyes030304 developmental biologychemistry.chemical_classification0303 health sciencesLiposomeBinding SitesMultidisciplinarybiologyAminoacridinesCell MembraneBiological activityPlants[SDV] Life Sciences [q-bio]Proton-Translocating ATPasesMembraneEnzymeSolubilitychemistryBiochemistryFusicoccinLiposomesbiology.proteinResearch Article010606 plant biology & botany
researchProduct

Granulation and microbial community dynamics in the chitosan-supplemented anaerobic treatment of wastewater polluted with organic solvents.

2018

Abstract The effect of chitosan on the development of granular sludge in upflow anaerobic sludge blanket reactors (UASB) when treating wastewater polluted with the organic solvents ethanol, ethyl acetate, and 1-ethoxy-2-propanol was evaluated. Three UASB reactors were operated for 219 days at ambient temperature with an organic loading rate (OLR) of between 0.3 kg COD m −3 d −1 and 20 kg COD m −3 d −1 . One reactor was operated without the addition of chitosan, while the other two were operated with the addition of chitosan doses of 2.4 mg gVSS −1 two times. The three reactors were all able to treat the OLR tested with COD removal efficiencies greater than 90%. However, the time required to…

0106 biological sciencesAigua ContaminacióEnvironmental EngineeringPolymersEthyl acetate010501 environmental sciencesWastewater01 natural sciencesWaste Disposal FluidMethanosaetaMethanomicrobialesChitosanchemistry.chemical_compoundGranulationExtracellular polymeric substanceBioreactors010608 biotechnologyAnaerobiosisParticle SizeWaste Management and Disposal0105 earth and related environmental sciencesWater Science and TechnologyCivil and Structural EngineeringBiological Oxygen Demand AnalysisChitosanbiologySewageEcological ModelingMicrobiotaGranule (cell biology)biology.organism_classificationPulp and paper industryPollutionMethanogenchemistryWastewaterSolventsAigua MicrobiologiaGeobacterWater Pollutants ChemicalWater research
researchProduct

Climatic influence on the growth pattern ofPanthasaurus maleriensisfrom the Late Triassic of India deduced from paleohistology

2020

Metoposaurids are representatives of the extinct amphibian clade Temnospondyli, found on almost every continent exclusively in the Late Triassic deposits. Osteohistologically, it is one of the best-known temnospondyl groups, analyzed with a wide spectrum of methods, such as morphology, morphometry, bone histology or computed modelling. The least known member of Metoposauridae isPanthasaurus maleriensisfrom the Pranhita-Godavari basin in Central India, being geographically the most southern record of this family. For the first time the bone histology of this taxon was studied with a focus on the intraspecific variability of the histological framework and the relationship between the observed…

0106 biological sciencesAmphibian010506 paleontologyHistologyOntogenylcsh:MedicineMetoposaurus010603 evolutionary biology01 natural sciencesGeneral Biochemistry Genetics and Molecular BiologyIntraspecific competitionPaleontologyTemnospondyliMetoposauridaebiology.animalCortex (anatomy)medicine0105 earth and related environmental sciencesPanthasaurus maleriensisHistovariabilitybiologyLate triassicGeneral Neurosciencelcsh:RPaleohistologyPaleontologyTemnospondyliClimatic influenceGeneral Medicinebiology.organism_classificationTaxonmedicine.anatomical_structureGeneral Agricultural and Biological SciencesZoologyPeerJ
researchProduct

2018

0106 biological sciencesAtmospheric Science010504 meteorology & atmospheric sciencesEcology010604 marine biology & hydrobiologyPaleontologySoil Sciencechemistry.chemical_elementForestryAquatic Science01 natural sciencesOxygenchemistry.chemical_compoundchemistryTotal inorganic carbonBorealEffects of global warmingEnvironmental chemistryDissolved organic carbonCarbon dioxideEnvironmental scienceEffluxWater quality0105 earth and related environmental sciencesWater Science and TechnologyJournal of Geophysical Research: Biogeosciences
researchProduct