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Generation of homozygosity and genome fixation in pea (Pisum sativum L.)
2013
Pea cultivars are nearly homozygous and thus homogeneous when they are released. The traditional method of selfing is slow and inefficient, taking up to ten generations of inbreeding following a cross to achieve a high level of homozygosity. Current single-seed-descent (SSD) methodologies enable a maximum of three generations per year to be developed in pea. Doubled haploidy and an in vitro based modified SSD technology have been utilised in many important crops for the rapid achievement of homozygosity, and thus acceleration of the breeding process. In pea, due to the lack of robust protocols, none of these technologies is routinely used in a breeding program. The aim of this study was to …