Search results for "focal"

showing 3 items of 803 documents

Human Papillomavirus Types 16, 18, and 31 Share Similar Endocytic Requirements for Entry

2013

ABSTRACT Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.

virusesImmunologyEndocytic cycleTetraspanin 24EndocytosisMicrobiologyClathrinDynamin IIPolymerizationDynamin IIMembrane MicrodomainsTetraspaninVirologyCaveolinHumansHuman papillomavirus 31Lipid raftDynaminHuman papillomavirus 16Microscopy ConfocalHuman papillomavirus 18biologyvirus diseasesLysosome-Associated Membrane GlycoproteinsVirus InternalizationVirologyActinsEndocytosisVirus-Cell InteractionsCell biologyMicroscopy ElectronMicroscopy FluorescenceInsect Sciencebiology.proteinElectrophoresis Polyacrylamide GelHeLa CellsJournal of Virology
researchProduct

Baculovirus-mediated immediate-early gene expression and nuclear reorganization in human cells

2007

Baculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), has the ability to transduce mammalian cell lines without replication. The general objective of this study was to detect the transcription and expression of viral immediate-early genes in human cells and to examine the interactions between viral components and subnuclear structures. Viral capsids were seen in large, discrete foci in nuclei of both dividing and non-dividing human cells. Concurrently, the transcription of viral immediate-early transregulator genes (ie-1, ie-2) and translation of IE-2 protein were detected. Quantitative microscopy imaging and analysis showed that virus transduction altered the size of …

virusesImmunologyGene ExpressionAnthraquinonesMicrobiologyCell LineHistonesMiceViral ProteinsTransduction (genetics)CapsidViral entryTranscription (biology)VirologyAnimalsHumansInsect virusGenes Immediate-EarlyGeneCell NucleusMicroscopy ConfocalbiologyChromatin Assembly and DisassemblyMolecular biologyNucleopolyhedrovirusesChromatinHistoneMicroscopy Fluorescencebiology.proteinImmediate early geneCellular Microbiology
researchProduct

Conception en technologie CMOS d'un Système de Vision dédié à l'Imagerie Rapide et aux Traitements d'Images

2008

Our work presented in this thesis focuses on the design, testing and implementation of monolithics CMOS image smart sensors : The principle, performance and limitations. The hardware implementation of a vision smart system is the central link. HISIC is High Speed Image Capture with processing at pixel level. An experimental platform for instrumentation and evaluation of retina operators was conducted during this thesis. After a state of the smart sensors and CMOS retinas, the second part is dedicated to the study and design of the pixel image sensor HISIC. Two circuits were realized in CMOS technology. The first identied a new type of photo-detector, and the second, to create a prototype em…

vision artificielleimagerie rapiderecongurabilityprogrammability[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronicsparallélisme massifspeed imagingSystème embarquéartificial vision.image processing on focal planerecongurabilitéprogrammabilitéstandard CMOS technology[ SPI.NANO ] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/MicroelectronicsEmbedded systemtraitements d'images sur plan focalmassive paralleltechnologie standard CMOS
researchProduct