Search results for "forme"

Nucleotide excision repair of abasic DNA lesions

2019

AbstractApurinic/apyrimidinic (AP) sites are a class of highly mutagenic and toxic DNA lesions arising in the genome from a number of exogenous and endogenous sources. Repair of AP lesions takes place predominantly by the base excision pathway (BER). However, among chemically heterogeneous AP lesions formed in DNA, some are resistant to the endonuclease APE1 and thus refractory to BER. Here, we employed two types of reporter constructs accommodating synthetic APE1-resistant AP lesions to investigate the auxiliary repair mechanisms in human cells. By combined analyses of recovery of the transcription rate and suppression of transcriptional mutagenesis at specifically positioned AP lesions, w…

Virulence and Molecular Typing of Vibrio harveyi Strains Isolated from Cultured Dentex, Gilthead Sea Bream and European Sea Bass

2003

Vibrio harveyi was isolated from internal organs or ulcers of diseased and apparently healthy gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax) cultured in several fish farms located on the Spanish Mediterranean coast. The prevalence of the bacterium was significantly higher in European sea bass than in gilthead sea bream, and was closely related to the season in both fish species, occurring almost exclusively on warm months (June to November). After phenotypic characterization, a selection of forty five isolates from gilthead sea bream, sea bass, and several isolates previously obtained from common dentex (Dentex dentex) of the same area, were molecularly type…

Description of Enterovibrio nigricans sp. nov., reclassification of Vibrio calviensis as Enterovibrio calviensis comb. nov. and emended description o…

2009

Eleven strains of halophilic, facultative anaerobes isolated from healthy and diseased Dentex dentex and Sparus aurata (bony fishes) cultured in Spanish Mediterranean fisheries have been studied by a polyphasic approach that included a wide phenotypic characterization, DNA-DNA hybridization and phylogenetic analysis using 16S rRNA, recA and rpoD gene sequences. All strains were phylogenetically related to Enterovibrio species and Vibrio calviensis. On the basis of sequence analysis and DNA-DNA hybridization data, eight of the strains were identified as Enterovibrio coralii. The remaining three strains formed a tight, independent clade in all sequence analyses and showed less than 70 % DNA-D…

The human p53 gene mutated at position 249per se is not sufficient to immortalize human liver cells

1999

A particular point mutation of the tumor suppressor gene p53, namely a G→T transversion at the third base of codon 249, is frequently detected in primary hepatocellular carcinomas from patients living in areas where the levels of dietary exposure to aflatoxin B 1 and the rates of infection with the hepatitis B virus are very high. Very recently, a nontumorigenic liver epithelial cell line (HACL-1) with a finite life-span and expressing a number of hepatocyte-specific markers was established from a human hepatocellular adenoma in our laboratory. To analyze the role of mutated p53 in the immortalization of human liver cells, we transfected HACL-1 cells with an expression vector containing a h…

Social transmission in the wild can reduce predation pressure on novel prey signals

2021

Funder: Suomen Kulttuurirahasto (Finnish Cultural Foundation); doi: https://doi.org/10.13039/501100003125

Fantasmi dell'isteria nella danza contemporanea

2008

Comparison between complete and simplified methods for determining the microwave noise parameters of HEMTs

1994

Modem automatic noise parameter measuring systems are based on measurements of some values of F(rs ) for different values of IГs (more than four, for redundancy) realized by adjusting an admittance transformer (tuner) inserted between the noise source and the TUT mput . Such values are then manipulated by means of proper data processing techniques using eqn.( 1) [ 11. This measuring procedure is hereafter referred to as standard to point out that the old `Standards on methods of measuring noise in linear twoports´ published by the IRE Subcommittee on noise in 1960 is considered obsolete by all the experimenters.

Datora ģenerēta dzeja

2022

Pēdējos gados ir bijuši vairāki mēģinājumi ar datora palīdzību automātiski ģenerēt arī ko radošu – vizuālās mākslas radīšana, radoša teksta ģenerēšana vai pat mūzikas skaņdarbu komponēšana – šīs ir tikai dažas no jomām, ar kurām darbojas mākslīgais intelekts. Dabiskās valodas ģenerēšana ir visai daudzsološa mākslīgā intelekta un datorlingvistikas apakšnozare, kuras galvenais mērķis ir izstrādāt datorprogrammas, kas spēj radīt cilvēkiem uztveramu tekstu. Starp automātiski ģenerētajiem teksta veidiem var atrast pat dažādas laika prognozes, biogrāfijas, kā arī tekstus, kas ietver sevī radošumu – dažādus stāstus, jokus vai dzeju. Automātiskā dzejas ģenerēšana ir šķietami sarežģīts uzdevums un ļ…

Dabiskās Valodas Dziļo Neironu Tīklu Ģeneratora GPT-2 Sniegums Latviešu un Angļu Valodā

2021

Darbs ir veltīts dabiskās valodas apstrādes tēmas un modeļa GPT-2 izpētei un tā snieguma novērtēšanai angļu valodā, kā arī pielāgota valodas modeļa realizēšanai un apmācībai tekstu ģenerēšanai arī latviešu valodā. GPT-2 ir liels dabiskās valodas apstrādes modelis, kas ir paplašināts no GPT modeļa, daudzkārt palielinot modeļa parametru skaitu un apmācot to uz vairāk treniņa datiem. GPT-2 modeļa apmācībai izmantots valodas korpuss no interneta mājaslapu tekstiem, atšķirībā no GPT modeļa, kura apmācībai izmantots valodas korpuss no grāmatu tekstiem. Šis modelis ir radīts ar mērķi paredzēt nākamo vārdu, ņemot vērā iepriekš esošos vārdus konkrētā tekstā. Apstrādājot iepriekš dotu tekstu, ar GPT-…

Protocol of a multicenter international randomized controlled manikin study on different protocols of cardiopulmonary resuscitation for laypeople (MA…

2018

IntroductionOut-of-hospital cardiac arrest is one of the leading causes of death in industrialised countries. Survival depends on prompt identification of cardiac arrest and on the quality and timing of cardiopulmonary resuscitation (CPR) and defibrillation. For laypeople, there has been a growing interest on hands-only CPR, meaning continuous chest compression without interruption to perform ventilations. It has been demonstrated that intentional interruptions in hands-only CPR can increase its quality. The aim of this randomised trial is to compare three CPR protocols performed with different intentional interruptions with hands-only CPR.Methods and analysisThis is a prospective randomise…