Search results for "forme"

Ethics appraisal procedure in 79,670 Marie Skłodowska-Curie proposals from the entire European HORIZON 2020 research and innovation program (2014–202…

2021

Introduction Horizon 2020 was the most significant EU Research and Innovation programme ever implemented and included the Marie Skłodowska-Curie Actions (MSCA). Proposals submitted to the MSCA actions awere subject to the Ethics Appraisal Procedure. In this work we explored the ethics appraisal procedure in MSCA H2020. Methods Using a retrospective analysis of pooled anonymized data, we explored the ethics appraisal procedure on proposals submitted to Marie Skłodowska-Curie Actions (MSCA) during the entire Horizon 2020 program period (N = 79,670). Results Our results showed that one of the most frequently identified ethics categories was Data protection. We also detected slight differences…

Empirically based analysis of methodological and ethical challenges in research with children as participants: the case of bullying in kindergarten

2015

ABSTRACTWhen conducting research with children it is essential to consider not only the data, which have been produced as a result of the research, but also the research process itself. This article represents an attempt to contribute the accumulation of knowledge regarding methodological and ethical issues concerning research with children. The data in this article are based on individual and focus-group interviews with children where ethical and methodical challenges related to dealing with children's experiences in the context of bullying in kindergarten are discussed. The topics that will be discussed related to methodical issues are (1) the nature of interaction between the researcher …

Dalla bacheca di Facebook alla città. Il primo atto della campagna contro una mostra di Hermann Nitsch

2016

Hermann Nitsch è un artista austriaco, considerato uno dei massimi esponenti dell’Azionismo Viennese (Wiener Aktionismus). La sua lunga carriera d’artista, di primo livello nella scena internazionale, incontra Palermo per via di una mostra approdata in città da Buenos Aires, città in cui era stata cancellata dopo le proteste. La mostra è stata duramente contestata anche a Palermo a partire da una petizione online per la sua cancellazione, tenuta sulla piattaforma change.org. Responsabile della protesta è stato un anonimo cittadino indignato per le pratiche ritenute crudeli nei confronti degli animali durante le performance dell’artista. La petizione ha raggiunto quota 70.000 adesioni, gener…

LOMO sapiens: de la photographie

2015

Une des questions les plus fréquentes, dans les recherches sur la photographie, est liée à la valeur de médiation de l’image et donc au rapport qu’elle entretient avec la soi-disant réalité. D’un côté, la certitude de la présence devant l’objectif de ce que nous voyons représenté, une copie du monde excluant la nécessité d’un code pour être comprise, de l’autre, l’imposition d’un point de vue situé dans le temps et l’espace qui, par ces choix obligés, porte en soi le germe d’une stratégie de la communication. La sémiotique devient ainsi l’instrument pour analyser ces stratégies, pour rechercher des signifiés plus ou moins cachés, en démontant l’évidence supposée de l’image photographique. D…

Overexpression of apolipoprotein J in human fibroblasts protects against cytotoxicity and premature senescence induced by ethanol and tert-butylhydro…

2008

Human diploid fibroblasts (HDFs) exposed to subcytotoxic stresses under H2O2, tert-butylhydroperoxide (t-BHP), and ethanol (EtOH) undergo stress-induced premature senescence (SIPS) characterized by many biomarkers of HDFs replicative senescence. Among these biomarkers are a growth arrest, an increase in the senescence-associated β-galactosidase activity, a senescent morphology, an overexpression of p21waf-1 and the subsequent inability to phosphorylate pRb, the presence of the common 4977-bp mitochondrial deletion, and an increase in the steady-state level of several senescence-associated genes such as apolipoprotein J (apo J). Apo J has been described as a survival gene against cytotoxic s…

Changes associated with aging and replicative senescence in the regulation of transcription factor nuclear factor-kappa B.

1996

Both the aging of animals and the senescence of cultured cells involve an altered pattern of gene expression, suggesting changes in transcription factor regulation. We studied age-related changes in transcription factors nuclear factor (NF)-kappa B, activator protein factor-1 (AP-1) and Sp-1 by using electrophoretic mobility shift binding assays; we also analysed changes in the protein components of NF-kappa B complex with Western blot assays. Nuclear and cytoplasmic extracts were prepared from heart, liver, kidney and brain of young adult and old NMRI mice and Wistar rats as well as from presenescent, senescent and simian virus 40-immortalized human WI-38 fibroblasts. Aging of both mice an…

Attenuation of NF-κB Signaling Response to UVB Light during Cellular Senescence

1999

The ability of cells to adapt to environmental stresses undergoes a progressive reduction during aging. NF-kappaB-mediated signaling is a major defensive system against various environmental challenges. The aim of this study was to find out whether replicative senescence affects the response of the NF-kappaB signaling pathway to UVB light in human WI-38 and IMR-90 fibroblasts. The exposure of early passage fibroblasts to UVB light inhibited the proliferation and induced a flat phenotype similar to that observed in replicatively senescent fibroblasts not exposed to UVB light. The UVB radiation dose used (153 mJ/cm2) did not induce apoptosis in either early or late passage WI-38 fibroblasts. …

Potentially human-virulent Vibrio vulnificus isolates from diseased great pompano (Trachinotus goodei).

2019

Vibrio vulnificus is an opportunistic human pathogen responsible for the majority of seafood-associated deaths worldwide and is also a relevant fish pathogen for the aquaculture industry. In addition to infections in aquatic livestock, V. vulnificus also represents a risk to aquarium animals. For the first time, this work describes an important mortality outbreak in Trachinotus goodei in a zoo aquarium, with the isolation of Vibrio vulnificus (Vv) from the internal organs of the diseased fish. The isolates were identified by MALDI-TOF MS, serotyped and characterized by pulsed-field gel electrophoresis (PFGE). Although the isolates from great pompanos did not belong to pathovar piscis (forme…

IDENTIFICAZIONE E UTILIZZAZIONE DI MARCATORI MOLECOLARI NELLA SISTEMATICA DEI FITOSEIDI (PARASITIFORMES, PHYTOSEIIDAE)

2012

Metilazione del DNA in artrite reumatoide

2005

Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…