Search results for "fusion"
showing 10 items of 4513 documents
Physics of the nuclear pore complex: Theory, modeling and experiment
2021
Abstract The hallmark of eukaryotic cells is the nucleus that contains the genome, enclosed by a physical barrier known as the nuclear envelope (NE). On the one hand, this compartmentalization endows the eukaryotic cells with high regulatory complexity and flexibility. On the other hand, it poses a tremendous logistic and energetic problem of transporting millions of molecules per second across the nuclear envelope, to facilitate their biological function in all compartments of the cell. Therefore, eukaryotes have evolved a molecular “nanomachine” known as the Nuclear Pore Complex (NPC). Embedded in the nuclear envelope, NPCs control and regulate all the bi-directional transport between the…
Uso pubblico della ragione e ruolo pubblico della Facoltà Filosofica nell'Illuminismo di Kant
2012
I filosofi accademici come contrappeso politico alla commerciabilità del sapere professionale; il "non accademico colto" nell'illuminismo di Kant.
La naturaleza estratégica del proceso de branding
2019
El branding, anglicismo con el que se denomina a la creación y gestión estratégica de una marca, cobró notoriedad en la década del ’90 incorporándose al debate académico y a la actividad profesional del campo disciplinar del diseño y el marketing. En ese trayecto provocó cierta confusión respecto a la naturaleza –a veces estratégica y a veces táctica– de la actividad. Este hecho da lugar a la reflexión teórica sobre el nuevo territorio y a explorar la actividad que rodea a la construcción de una marca, en un intento por descubrir perspectivas teóricas que ayuden a comprender el proceso. Al hacerlo, se examina la noción demarca como sistema bipolar de producción y proyección de imágenes, y s…
The Itô Integral
2014
The Ito integral allows us to integrate stochastic processes with respect to the increments of a Brownian motion or a somewhat more general stochastic process. We develop the Ito integral first for Brownian motion and then for generalized diffusion processes (so called Ito processes). In the third section, we derive the celebrated Ito formula. This is the chain rule for the Ito integral that enables us to do explicit calculations with the Ito integral. In the fourth section, we use the Ito formula to obtain a stochastic solution of the classical Dirichlet problem. This in turn is used in the fifth section in order to show that like symmetric simple random walk, Brownian motion is recurrent …
Production of Hev b5 as a fluorescent biotin-binding tripartite fusion protein in insect cells
2005
The presented green fluorescent protein and streptavidin core-based tripartite fusion system provides a simple and efficient way for the production of proteins fused to it in insect cells. This fusion protein forms a unique tag, which serves as a multipurpose device enabling easy optimization of production, one-step purification via streptavidin-biotin interaction, and visualization of the fusion protein during downstream processing and in applications. In the present study, we demonstrate the successful production, purification, and detection of a natural rubber latex allergen Hev b5 with this system. We also describe the production of another NRL allergen with the system, Hev b1, which fo…
Recombinant avidin and avidin-fusion proteins.
2000
Both chicken egg-white avidin and its bacterial relative streptavidin are well known for their extraordinary high affinity with biotin (Kd approximately 10(-15) M). They are widely used as tools in a number of affinity-based separations, in diagnostic assays and in a variety of other applications. These methods have collectively become known as (strept)avidin-biotin technology. Biotin can easily and effectively be attached to different molecules, termed binders and probes, without destroying their biological activity. The exceptional stability of the avidin-biotin complex and the wide range of commercially available reagents explain the popularity of this system. In order by genetic enginee…
Molecular Co-operation between Protein PAM and Streptokinase for Plasmin Acquisition by Streptococcus pyogenes
1998
Bacterial surface-associated plasmin formation is believed to contribute to invasion, although the underlying molecular mechanisms are poorly understood. To define the components necessary for plasmin generation on group A streptococci we used strain AP53 which exposes an M-like protein ("PAM") that contains a plasminogen-binding sequence with two 13-amino acid residues long tandem repeats (a1 and a2). Utilizing an Escherichia coli-streptococcal shuttle vector, we replaced a 29-residue long sequence segment of Arp4, an M-like protein that does not bind plasminogen, with a single (a1) or the combined a1a2 repeats of PAM. When expressed in E. coli, the purified chimeric Arp/PAM proteins both …
Mutations affecting MHC class II binding of the superantigen streptococcal erythrogenic toxin A.
1993
Streptococcal pyrogenic exotoxin A (SPEA) is an important pathogenicity factor of group A streptococci. It is a member of the family of 'superantigens' produced by Staphylococcus aureus and Streptococcus pyogenes, and its T lymphocyte stimulating activity is involved in the pathogenesis of certain diseases caused by pyogenic streptococci. In this study we have generated nine mutant SPEA molecules by substituting amino acids in the regions of homology between different streptococcal and staphylococcal superantigens. An additional mutant was created by deletion of the 10 N-terminal amino acids. The mutants were expressed as fusion proteins. Several mutations led to a loss of function due to a…
Effect of microcracking on pressure-solution strain rate: The Gratz grain-boundary model
1998
Different, but reasonable and well-accepted assumptions made about grain-boundary structure during pressure-solution (PS) creep may easily have an effect of more than 10 orders of magnitude on the calculated PS deformation rate. Understanding of grain-boundary structure during PS creep is therefore extremely important. Experimental evidence is presented in support of a grain-boundary model previously proposed by A. J. Gratz on the basis of observations on naturally deformed rocks. In this model, boundaries are assumed to have a static island-channel network structure. Channels are located where microcracks intersect the boundary. The rate of material transport is governed by thin-film diffu…
Formation of dislocation patterns: Computer simulations
1996
Dislocations patterns have been extensively studied by means of TEM. In parallel, theoretical approaches have been developed by using two methods; reaction diffusion schemes and computer simulation models. This distinction is not rigid since some computer models include the former approach in their evolution equations. Independently from the difficulties each approach presents in formulating the collective behavior of dislocations, the aim of these studies is to exhibit simple dislocation patterns as persistent slip bands and/or cellular organization. In this context, computer simulations brought a methodology which undoubtedly is a complement to the existing approaches for dislocations. Ne…