Search results for "fusion"

showing 10 items of 4513 documents

Matrix-mediated canal formation in primmorphs from the sponge Suberites domuncula involves the expression of a CD36 receptor-ligand system.

2004

Sponges (Porifera), represent the phylogenetically oldest metazoan phylum still extant today. Recently, molecular biological studies provided compelling evidence that these animals share basic receptor/ligand systems, especially those involved in bodyplan formation and in immune recognition, with the higher metazoan phyla. An in vitro cell/organ-like culture system, the primmorphs, has been established that consists of proliferating and differentiating cells, but no canals of the aquiferous system. We show that after the transfer of primmorphs from the demosponge Suberites domuncula to a homologous matrix (galectin), canal-like structures are formed in these 3D-cell aggregates. In parallel …

CD36 AntigensTime FactorsGalectinsRecombinant Fusion ProteinsAmino Acid MotifsMolecular Sequence DataGene ExpressionChick EmbryoLigandsEvolution MolecularDemospongeAllantoisSequence Analysis ProteinAnimalsAmino Acid SequenceCloning MolecularReceptorCells CulturedPhylogenyGalectinCell AggregationGlutathione TransferasebiologyDose-Response Relationship DrugMolecular StructureSequence Homology Amino AcidCell growthCell DifferentiationCell BiologyAnatomyChorionLigand (biochemistry)biology.organism_classificationIn vitroCell biologyExtracellular MatrixPoriferaProtein Structure TertiarySuberites domunculaSpongeThrombospondinsCell DivisionNaphthoquinonesJournal of cell science
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Donor CD4 T cells convert mixed to full donor T-cell chimerism and replenish the CD52-positive T-cell pool after alemtuzumab-based T-cell-depleted al…

2009

Donor lymphocyte infusions (DLI) are used to resolve mixed T-cell chimerism (TCC) after allo-SCT despite a substantial risk of GVHD. We analyzed the impact of prophylactic CD8-depleted (CD8(depl)) DLI in 20 recipients of anti-CD52 alemtuzumab in vivo T-cell-depleted allografts with declining donor TCC after day +60. A total of 13 patients received CD8(depl) DLI and 7 patients did not. All but one of the DLI patients converted to complete donor T-cell chimeras, whereas only one non-DLI patient converted spontaneously. DLI induced transient acute GVHD in five and extensive chronic GVHD in two patients. These data suggest the use of CD8(depl) DLI as an effective treatment for mixed TCC, partic…

CD4-Positive T-LymphocytesAntibodies NeoplasmLymphocytemedicine.medical_treatmentHematopoietic stem cell transplantation*Lymphocyte DepletionT-Lymphocyte SubsetsAlemtuzumabddc:616Transplantation Chimera/*immunologyHematopoietic Stem Cell TransplantationAntibodies MonoclonalHematologyMiddle Agedmedicine.anatomical_structureCD52 AntigenLymphocyte TransfusionAlemtuzumabmedicine.drug*GlycoproteinsAdultCD52T cellAntibodies Monoclonal/therapeutic useAntineoplastic AgentsCD4-Positive T-Lymphocytes/*transplantationAntibodies Monoclonal HumanizedLymphocyte DepletionAntigens CDAntigens NeoplasmmedicineAntibodies Neoplasm/therapeutic useHumans*Peripheral Blood Stem Cell TransplantationAgedCell ProliferationGlycoproteinsLymphocyte Transfusion/*methodsTransplantationPeripheral Blood Stem Cell TransplantationTransplantation Chimerabusiness.industryAntineoplastic Agents/therapeutic usemedicine.diseaseTransplantationGraft-versus-host disease*Antigens NeoplasmImmunology*Antigens CDbusinessCD8Bone marrow transplantation
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The gp130-stimulating designer cytokine hyper-IL-6 promotes the expansion of human hematopoietic progenitor cells capable to differentiate into funct…

2000

Abstract Objective . Hyper-IL-6, a fusion protein of interleukin-6 and its specific receptor, together with stem cell factor leads to the proliferation of primitive hematopoietic progenitor cells. Based on these findings, the current study examined whether hyper-IL-6 promotes the growth of precursor cells that can be further differentiated into dendritic cells in the presence of additional cytokines. Methods . Dendritic cell cultures were generated from CD34 + hematopoietic progenitor cells derived either from bone marrow or from peripheral blood. CD34 + cells were cultured in the presence of cytokines for 2 weeks and then used for phenotyping and T-cell stimulation assays. Results . Hyper-…

CD4-Positive T-LymphocytesCancer ResearchRecombinant Fusion ProteinsAntigen presentationBiologyDinoprostoneImmunophenotypingAntigens CDOxytocicsGeneticsCytokine Receptor gp130HumansProgenitor cellAntigen-presenting cellMolecular BiologyCells CulturedInterleukin 3Antigen PresentationStem Cell FactorMembrane GlycoproteinsFollicular dendritic cellsInterleukin-6Tumor Necrosis Factor-alphaGranulocyte-Macrophage Colony-Stimulating FactorCell DifferentiationCell BiologyHematologyDendritic cellDendritic CellsReceptors InterleukinFlow CytometryHematopoietic Stem CellsHepatitis B Core AntigensReceptors Interleukin-6Recombinant ProteinsCell biologyEndothelial stem cellMyeloid-derived Suppressor CellInterleukin-4Cell DivisionInterleukin-1Experimental hematology
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Regulation of Protein-DNA Interactions at the Interferon-gamma Gene Promoter by Corticosteroids: Implications for Inflammatory Bowel Diseases

1998

CD4-Positive T-LymphocytesRecombinant Fusion ProteinsProtein dnaInterferon-gamma biosynthesisTransfectionGeneral Biochemistry Genetics and Molecular BiologyInterferon-gammaHistory and Philosophy of ScienceAdrenal Cortex HormonesGenes ReporterT-Lymphocyte SubsetsmedicineHumansInterferon gammaPromoter Regions GeneticGenebusiness.industryGeneral NeuroscienceInflammatory Bowel DiseasesPromoterTransfectionInflammatory Bowel DiseasesTranscription Factor AP-1ImmunologyLeukocyte Common AntigensCancer researchLeukocyte Common Antigensbusinessmedicine.drugAnnals of the New York Academy of Sciences
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Local blockade of IL-6R signaling induces lung CD4+ T cell apoptosis in a murine model of asthma via regulatory T cells.

2007

We previously reported high levels of the soluble form of the IL-6R (sIL-6R) in the airways of asthmatic subjects. Here, we analyzed the IL-6R effects on Th2 cell survival in the lung by locally antagonizing sIL-6R-mediated trans-signaling with a designer fusion protein (gp130-Fc) as well as IL-6R signaling with an antibody against the gp80 unit of the IL-6R (alphaIL-6R) in a murine model of asthma after ovalbumin peptide (OVA) sensitization and challenge. Blockade of the sIL-6R led to a significant decrease in inflammatory cells by an apoptosis-independent mechanism. In contrast, local treatment with alphaIL-6R antibodies that also block signaling via the membrane-bound IL-6R (mIL-6R) led …

CD4-Positive T-LymphocytesSTAT3 Transcription FactorOvalbuminT cellRecombinant Fusion ProteinsImmunologyGene ExpressionApoptosisBiologyT-Lymphocytes RegulatoryAntibodiesInterleukin 21MicemedicineCytokine Receptor gp130Immunology and AllergyCytotoxic T cellAnimalsIL-2 receptorPhosphorylationLungMice Inbred BALB CInterleukin-6FOXP3Forkhead Transcription FactorsGeneral MedicineT lymphocyterespiratory systemReceptors Interleukin-6AsthmaCoculture TechniquesImmunoglobulin Fc FragmentsDisease Models Animalmedicine.anatomical_structureApoptosisImmunologyCancer researchFemaleImmunizationSignal transductionBronchoalveolar Lavage FluidSignal TransductionInternational immunology
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Increased antigen presentation efficiency by coupling antigens to MHC class I trafficking signals.

2007

Abstract Genetic modification of vaccines by linking the Ag to lysosomal or endosomal targeting signals has been used to route Ags into MHC class II processing compartments for improvement of CD4+ T cell responses. We report in this study that combining an N-terminal leader peptide with an MHC class I trafficking signal (MITD) attached to the C terminus of the Ag strongly improves the presentation of MHC class I and class II epitopes in human and murine dendritic cells (DCs). Such chimeric fusion proteins display a maturation state-dependent subcellular distribution pattern in immature and mature DCs, mimicking the dynamic trafficking properties of MHC molecules. T cell response analysis in…

CD4-Positive T-LymphocytesT cellRecombinant Fusion ProteinsImmunologyAntigen presentationMolecular Sequence DataMice Inbred StrainsCD8-Positive T-LymphocytesProtein Sorting SignalsMajor histocompatibility complexTransfectionViral Matrix ProteinsEpitopesMiceAntigens NeoplasmMHC class ImedicineImmunology and AllergyAnimalsHumansAmino Acid SequenceAntigensMHC class IIAntigen PresentationbiologyAntigen processingHistocompatibility Antigens Class IVaccinationMembrane ProteinsDendritic CellsMHC restrictionPhosphoproteinsCell biologyProtein Transportmedicine.anatomical_structurebiology.proteinCD8Journal of immunology (Baltimore, Md. : 1950)
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Regulation of Protein-DNA Interactions at the Interferon-gamma Gene Promoter by Corticosteroids

1998

CD4-Positive T-LymphocytesTranscription GeneticRecombinant Fusion ProteinsProtein dnaBiologyLymphocyte ActivationTransfectionDexamethasoneGeneral Biochemistry Genetics and Molecular BiologyInterferon-gammaHistory and Philosophy of ScienceAdrenal Cortex HormonesAntigens CDGenes ReportermedicineHumansInterferon gammaInterleukin 29Promoter Regions GeneticCells CulturedGeneral NeurosciencePromoterTATA BoxMolecular biologyTranscription Factor AP-1Cancer researchLeukocyte Common AntigensTetradecanoylphorbol Acetatemedicine.drugAnnals of the New York Academy of Sciences
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The blood perfusion mapping in the human skin by photoplethysmography imaging

2010

A CMOS camera-based imaging photoplethysmographic (PPGI) system is described to detect the blood pulsations in tissue. Attention of PPGI is drawn to the potential applications in visualized blood perfusion. Intensity variations of three wavelengths (620 nm, 520 nm and 432 nm) were detected and analyzed in each pixel of image. To obtain a twodimensional mapping of the dermal perfusion measurement, custom image-processing software has been developed. The high-resolution PPGI images were derived from human fingers (transmission mode) and face (reflection mode), evaluated at three wavelengths. The newly developed system can be usable in skin blood perfusion monitoring for clinical applications.

CMOS sensorPixelbusiness.industryPhotoplethysmogramMedicineHuman skinbusinessPerfusionBiomedical engineeringIntensity (physics)
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Gravi infezioni del virus della “tristezza” degli agrumi (CTV) mettono a rischio l’agrumicoltura calabrese

2007

CTV diffusioneSettore AGR/12 - Patologia Vegetale
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Modelling Cd(II) removal from aqueous solutions by adsorption on a highly mineralized peat. Batch and fixed-bed column experiments

2006

This paper evaluates the potential use of a locally available organic soil amendment as a low-cost adsorbent. The removal of cadmium from aqueous solutions was studied by means of kinetic, batch and fixed-bed experiments. Batch experiments were conducted to evaluate the process kinetics and the removal equilibrium over a broad pH range. Pseudo-second-order kinetics and Freundlich equilibrium parameters were obtained. Six column experiments were carried out at different flow-rates and feed concentrations. Breakthrough curves showed higher metal retention than expected from the batch adsorption isotherms. Column modelling assuming rate-controlled pore diffusion was successfully performed. The…

CadmiumAqueous solutionChromatographyRenewable Energy Sustainability and the EnvironmentGeneral Chemical EngineeringDiffusionOrganic ChemistryKineticsAmendmentchemistry.chemical_elementHydrochloric acidPollutionInorganic Chemistrychemistry.chemical_compoundFuel TechnologyAdsorptionchemistryChemical engineeringFreundlich equationWaste Management and DisposalBiotechnologyJournal of Chemical Technology & Biotechnology
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