Search results for "gel"

Hydrogel‐Based 3D Bioprinting for Bone and Cartilage Tissue Engineering

2020

As a milestone in soft and hard tissue engineering, a precise control over the micropatterns of scaffolds has lightened new opportunities for the recapitulation of native body organs through three dimentional (3D) bioprinting approaches. Well-printable bioinks are prerequisites for the bioprinting of tissues/organs where hydrogels play a critical role. Despite the outstanding developments in 3D engineered microstructures, current printer devices suffer from the risk of exposing loaded living agents to mechanical (nozzle-based) and thermal (nozzle-free) stresses. Thus, tuning the rheological, physical, and mechanical properties of hydrogels is a promising solution to address these issues. Th…

Comparison of Diatoms and Dinoflagellates from Different Habitats as Sources of PUFAs

2019

Recent studies have clearly shown the importance of omega-3 (&omega

Postharvest Application of Aloe vera Gel-Based Edible Coating to Improve the Quality and Storage Stability of Fresh-Cut Papaya

2020

Ready-to-eat products are damaged by various factors, including exposure to O2 and CO2, extreme temperatures, and rapid decay, due to trauma during processing. The use of natural antimicrobial agents and antioxidants might extend the shelf-life of the fruits. The aim of this work is to investigate the effects of four different antibrowning and gelling agents added into the Aloe vera gel-based edible coatings and applied to fresh-cut papaya. EC1 treatment consists of Aloe vera gel (30% v/v), EC2 contains CaCl2 (5% v/v), EC3 contains K carrageenan (0.5% v/v), and EC4 contains sodium alginate (1.5% v/v) and K carrageenan (0.5% v/v). The fruits treated with EC2 showed the best results while mai…

Plankton Tracker: A novel integrated system to investigate the dynamic sinking behavior in phytoplankton

2020

Abstract Phytoplankton sinking is an important property that can determine community composition, affecting nutrient and light absorption in the photic zone, and influencing biogeochemical cycling via material loss to the deep ocean. To date, the difficulty in exploring the sinking processes is partly due to methodological limitations in measuring phytoplankton sinking rate. However, in the last decade, works have illustrated various methods based on some non-invasive and low perturbing approaches (laser scanner, video-microscopy, fluorescence spectroscopy). In this study, we review the methods for sinking rate estimation and describe the Plankton Tracker, a novel integrated system to inves…

The shell matrix of the pulmonate land snail Helix aspersa maxima.

2012

12 pages; International audience; In mollusks, the shell mineralization process is controlled by an array of proteins, glycoproteins and polysaccharides that collectively constitute the shell matrix. In spite of numerous researches, the shell protein content of a limited number of model species has been investigated. This paper presents biochemical data on the common edible land snail Helix aspersa maxima, a model organism for ecotoxicological purposes, which has however been poorly investigated from a biomineralization viewpoint. The shell matrix of this species was extracted and analyzed biochemically for functional in vitro inhibition assay, for amino acid and monosaccharides composition…

Larger cell or colony size in winter, smaller in summer – a pattern shared by many species of Lake Kinneret phytoplankton

2017

We examined an 8.5-year record (2004-2012) of cell size data for phytoplankton species from Lake Kinneret, Israel, sampled weekly or at 2-week intervals and determined microscopically by the same person. Many of the species abundant enough to be counted year-round showed a typical seasonal cell size pattern that repeated annually: cell diameter was maximal in winter and minimal in summer. This pattern was shared by species from different taxonomic groups including cyanobacteria, chlorophyta, and dinoflagellates. Similarly, in colonial species of diatoms, chlorophyta, and cyanobacteria the number of cells per colony was larger in winter and smaller in summer. We postulated that the seasonal …

Chromatographic separation and partial identification of glycosidically bound volatile components of fruit

1990

Abstract Synthetic monoterpenic and aromatic β- d -glucosides and β- d -rutinosides were separated by Fractogel TSK HW-40 S chromatography according to their molecular size and interactions occurring between the aglycone moiety and the gel matrix. Under these conditions terpenyl rutinosides were eluted before the homologous glucoside derivatives. In the two classes, monoglucosides and rutinosides, aromatic glycosidically bound components had lower retention times than the corresponding terpenyl components. The use of over-pressure layer chromatography (OPLC) allowed the separation of glucoside and rutinoside derivatives, and in these two classes aromatic and several monoterpene compounds we…

2020

Phytoplankton synthesizes essential ω-3 and ω-6 polyunsaturated fatty acids (PUFA) for consumers in the aquatic food webs. Only certain phytoplankton taxa can synthesize eicosapentaenoic (EPA; 20:5ω3) and docosahexaenoic acid (DHA; 22:6ω3), whereas all phytoplankton taxa can synthesize shorter-chain ω-3 and ω-6 PUFA. Here, we experimentally studied how the proportion, concentration (per DW and cell-specific), and production (µg FA L−1 day−1) of ω-3 and ω-6 PUFA varied among six different phytoplankton main groups (16 freshwater strains) and between exponential and stationary growth phase. EPA and DHA concentrations, as dry weight, were similar among cryptophytes and diatoms. However, Crypto…

Subcellular localization and purification of a p-hydroxyphenylpyruvate dioxygenase from cultured carrot cells and characterization of the correspondi…

1997

p-Hydroxyphenylpyruvate dioxygenase catalyses the transformation of p-hydroxyphenylpyruvate into homogentisate. In plants this enzyme has a crucial role because homogentisate is the aromatic precursor of all prenylquinones. Furthermore this enzyme was recently identified as the molecular target for new families of potent herbicides. In this study we examine precisely the localization of p-hydroxyphenylpyruvate dioxygenase activity within carrot cells. Our results provide evidence that, in cultured carrot cells, p-hydroxyphenylpyruvate dioxygenase is associated with the cytosol. Purification and SDS/PAGE analysis of this enzyme revealed that its activity is associated with a polypeptide of 4…

Innovative food processing technologies on the transglutaminase functionality in protein-based food products: Trends, opportunities and drawbacks

2018

Abstract Background Consumption of protein-based food products has a key role in the improvement of human health. The crosslinking agent microbial transglutaminase (mTGase) is an effective and promising tool to modify animal proteins used in the food industry. Improvement in the gelation process, physicochemical and textural quality, and consumer's demand of protein-based food products could be attained by combining mTGase and some non-conventional food processing technologies. Scope and approach New perspectives and key areas for future research in the development of high-quality food proteins and protein-based products as a function of interaction effect of mTGase and some new processing …