Search results for "globin"

Protection of islets in culture by delivery of oxygen binding neuroglobin via protein transduction.

2005

Islet transplantation has become an accepted method to treat type 1 diabetes. To succeed and achieve normal levels of glucose in transplant recipients, the quality of the transplanted islets is of the utmost importance. Lack of oxygen during organ procurement, islet isolation, and subsequent culture triggers apoptosis or necrosis and loss of islet function, causing the yield and quality to diminish. A promising candidate for cytoprotection against oxygen deprivation is neuroglobin (Ngb). Ngb is a recently described member of globin family and is expressed in neurons, retina, and pancreatic islets. To overexpress this protein in the islets and study its ability to protect them, we utilized p…

Oxygen parameters of blood: Definitions and symbols

1990

Definitions and symbols for relevant parameters of the oxygen status of arterial blood are recommended. The recommendations are as simple as possible, easy to understand, and devoid of misinterpretations and double meanings. The authors propose no new definitions for limited new methods, no combination of symbols and methods, and no association between definition, symbol and commercial name.

Cooperativity of Protein Binding to Vesicles

2011

Electrostatics role is studied in protein adsorption to phosphatidylcholine (PC) and PC/phosphatidylglycerol (PG) small unilamellar vesicles (SUVs). Protein interaction is monitored vs. PG content at low ionic strength. Adsorption of lysozyme, myoglobin and bovine serum albumin (BSA) isoelectric point (pI) is investigated in SUVs, along with changes in protein fluorescence emission spectra. Partition coefficients and cooperativity parameters are calculated. At pI, binding is maximum while at lower/higher pHs binding drops. In Gouy–Chapman model activity coefficient goes with square charge number, which deviations indicate asymmetric location of anionic lipid in the bilayer inner leaflet, in…

Adaptations to long-term apnoea training

2010

Myelotoxicity from cytostatic treatment, an important risk factor in the management of neoplasias in the elderly

2006

Myelotoxicity is the most common complication of cancer chemotherapy. A brief review of hematopoiesis is necessary to understand the mechanisms of myelotoxicity and the treatment strategies that may minimize this complication in elderly patients. From this review of hematopoiesis and age, we may draw three conclusions: aging is associated with a progressive reduction in hematopoietic reserve; by itself, the decline in hematopoietic reserve does not produce abnormalities of the circulating blood, these may occur as a consequence of hematopoietic stress; hematopoietic growth factors are effective in promoting granulopoiesis and erythropoiesis in older individuals. Alternative strategies inclu…

PREDICTING BLADDER CANCER AT THE EMERGENCY UNIT: THE IMPACT OF HEMOGLOBIN VALUE

2014

Aim of the study Haematuria represents the most common symptom at presentation in patients with bladder Cancer (BCa). However, no study has tested the predictors of BCa in patients presenting with haematuria. This prospective study was aimed to assess the factors associated with the presence of BCa in patients presenting at our emergency unit due to gross-hematuria. Materials and methods All patients presenting at the emergency unit of our institution for their first episode of gross haematuria between January and December 2012 were evaluated. All clinical patient characteristics, including the ongoing antiplatelets and/or oral anticoagulative treatment, were recorded. Demographical, hemato…

Correlations of blood selenium with hematological parameters in West German adults.

1988

The serum selenium and the whole blood selenium of 72 healthy persons (47 women, 25 men) was determined. There exist sex specific differences of the whole blood selenium between men (98 +/- 19 micrograms Se/L) and women (89 +/- 17 micrograms Se/L). The serum selenium did not show sex specific differences, but sex specific differences are found if the total amount of extracellular selenium is calculated by correction of the serum selenium with the hematocrit. Women have more extra-cellular selenium/L whole blood (40 +/- 8 micrograms Se) than men (36 +/- 7 micrograms Se). Men have more intraerythrocyte selenium (cellular selenium = 67 +/- 14 micrograms Se) in one L whole blood than women (52 …

<title>Micro-Raman scattering and infrared spectra of hemoglobin</title>

2008

Confocal micro-Raman and FT-IR spectroscopies have been used for detection of radiation influence of hemoglobin of patients examined by radio-isotopes diagnosis (Tc99m). After irradiation we observed some little changes of the Raman scattering bands which connected with out of plane porphyrine bending vibrations, also we observed additional band due to methemoglobin. Radiation of blood lead to the transition from hemoglobin (Fe2+) to methemoglobin (Fe3+) with a delocalization of iron from porphyrine plane. It was shown that FT-IR spectra indicate the radiation effects on hemoglobin.© (2008) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is per…

Hydration dependence of myoglobin dynamics studied with elastic neutron scattering, differential scanning calorimetry and broadband dielectric spectr…

2014

In this work we present a thorough investigation of the hydration dependence of myoglobin dynamics. The study is performed on D2O-hydrated protein powders in the hydration range 0<h<0.5 (h≡gr[D2O]/gr[protein]) and in the temperature range 20-300K. The protein equilibrium fluctuations are investigated with Elastic Neutron Scattering using the spectrometer IN13 at ILL (Grenoble), while the relaxations of the protein + hydration water system are investigated with Broadband Dielectric Spectroscopy; finally, Differential Scanning Calorimetry is used to obtain a thermodynamic description of the system. The effect of increasing hydration is to speed up the relaxations of the myoglobin + hydration …

Globins and hypoxia adaptation in the goldfish, Carassius auratus

2008

Goldfish (Carassius auratus) may survive in aquatic environments with low oxygen partial pressures. We investigated the contribution of respiratory proteins to hypoxia tolerance in C. auratus. We determined the complete coding sequence of hemoglobin α and β and myoglobin, as well as partial cDNAs from neuroglobin and cytoglobin. Like the common carp (Cyprinus carpio), C. auratus possesses two paralogous myoglobin genes that duplicated within the cyprinid lineage. Myoglobin is also expressed in nonmuscle tissues. By means of quantitative real-time RT-PCR, we determined the changes in mRNA levels of hemoglobin, myoglobin, neuroglobin and cytoglobin in goldfish exposed to prolonged hypoxia (48…