Search results for "grape"
showing 10 items of 291 documents
Highly sensitive monoclonal antibody-based immunoassays for the analysis of fluopyram in food samples
2019
Monoclonal antibody-based techniques have become a useful analytical technology in the agro-food sector. Nowadays, residues of the recently registered fungicide fluopyram are increasingly being found in quality control programs. In the present study, novel chemical derivatives of this pesticide were prepared and specific and high-affinity monoclonal antibodies to fluopyram were raised for the first time. Moreover, immunoassays to fluopyram were developed in two alternative enzyme-linked immunosorbent assay formats, using homologous and heterologous assay conjugates, with limits of detection below 0.05 µg L−1. The optimized immunoassays were applied to the analysis of fluopyram in fortified …
Interplay among Gcn5, Sch9 and mitochondria during chronological aging of wine yeast is dependent on growth conditions.
2015
Saccharomyces cerevisiae chronological life span (CLS) is determined by a wide variety of environmental and genetic factors. Nutrient limitation without malnutrition, i.e. dietary restriction, expands CLS through the control of nutrient signaling pathways, of which TOR/Sch9 has proven to be the most relevant, particularly under nitrogen deprivation. The use of prototrophic wine yeast allows a better understanding of the role of nitrogen in longevity in natural and more demanding environments, such as grape juice fermentation. We previously showed that acetyltransferase Gcn5, a member of the SAGA complex, has opposite effects on CLS under laboratory and winemaking conditions, and is detrimen…
Monitoring soil volume wetness in heterogeneous soils by electrical resistivity. A field-based pedotransfer function.: A field-based pedotransfer fun…
2014
11 pages; International audience; Modern irrigation techniques require accurate, rapid, cost-effective, spatial measurement of soil moisture. Electrical Resistivity Tomography (ERT) meets most of these requirements, but needs to be calibrated for each use because it is very sensitive to differences in soil characteristics. In this study, a pedotransfer function approach is used to remove the need for site-specific calibration, allowing ERT to be used directly to measure soil moisture. The study site was a hillslope vineyard, where eight calcaric-cambisol soil profiles were identified. From 2012 to 2013, 23 000 soil volume wetness measurements were acquired by Time Domain Reflectometry, and …
Comparison of SWAP and FAO Agro-Hydrological Models to Schedule Irrigation of Wine Grapes
2012
This paper compares two agro-hydrological models that are used to schedule irrigation of a typical Mediterranean crop. In particular, a comparison between the Food and Agriculture Organization (FAO) model, which uses a black box approach, and the soil-water-atmosphere-plant (SWAP) model, which is based on the numerical analysis of Richards' equation, are shown for wine grape. The comparison was carried out for the 2005 and 2006 irrigation seasons and focused on hydrological balance components and on soil water contents. Next, the ordinary scheduling parameters were identified so that the performance of the two models, which aimed to evaluate the seasonal water requirements and the irrigatio…
Application of the SWAP model to predict impact of climate change on soil water balance in a Sicilian vineyard
2012
In Sicily, the increasing scarcity of quality water is leading to irrigation with saline water in soils having a considerable susceptibility to salinization and degradation. In this paper, water flow and solute transport in a Sicilian irrigated soil was simulated by using the SWAP model, and the simulated results compared to measured values of soil moisture and salinity. The investigation was carried out in a vineyard located in Sicily (Mazara del Vallo, Trapani Province) within the framework of the Project: Evolution of cropping systems as affected by climate change (CLIMESCO). The soil hydraulic parameters were obtained by inverse method based on multi-step outflow experiments. The result…
Food-mediated modulation of immunity in a phytophagous insect: An effect of nutrition rather than parasitic contamination.
2015
7 pages; International audience; Inherent to the cost of immunity, the immune system itself can exhibit tradeoffs between its arms. Phytophagous insects face a wide range of microbial and eukaryotic parasites, each activating different immune pathways that could compromise the activity of the others. Feeding larvae are primarily exposed to microbes, which growth is controlled by antibiotic secondary metabolites produced by the host plant. The resulting variation in abundance of microbes on plants is expected to differentially stimulate the insect antimicrobial immune defenses. Under the above tradeoff hypothesis, stimulation of the insect antimicrobial defenses is expected to compromise imm…
Cellulolytic bacteria joined with deproteinized whey decrease carbon to nitrogen ratio and improve stability of compost from wine production chain by…
2022
Composting residues from wine and dairy chains would contribute to increase the environmental sustainability of the production. The aim of this study was to evaluate the effects of deproteinized whey combined with bioactivators on the composting process. Bacillus velezensis and Kocuria rhizophila, bacteria with cellulolytic activity, were isolated from raw materials and inoculated in the organic mass to be composted. Piles moistened with deproteinized whey showed the highest reduction of total and dissolved organic carbon due to the stimulation of bacterial activity by nitrogen compounds held within deproteinized whey. Such findings were also confirmed by the speed up of the microbial carbo…
Biodiversity and dairy traits of indigenous milk lactic acid bacteria grown in presence of the main grape polyphenols
2020
ABSTRACT The present work was developed to select lactic acid bacteria (LAB) to be used as starter cultures in functional cheese production. The indigenous milk LAB populations were isolated from fermented raw ewes’ milks (four bulks) added with 0.5 mg/mL of nine polyphenols commonly found in winery by-products. After 48 h of fermentation, all milks were characterized by an increase of LAB levels of about 3–4 Log cycles. All different colonies were purified and characterized for the main physiological and biochemical traits and then differentiated genetically at strain level and identified. Ten species belonging to the LAB genera Lactobacillus, Streptococcus, Enterococcus, Leuconostoc and L…
Infection of Bois-Noir tuf-type-I stolbur phytoplasma inHyalesthes obsoletus(Hemiptera: Cixiidae) larvae and influence on larval size
2009
Recent dramatic spread of the grapevine yellows disease Bois Noir (BN) in Germany is above all explained by highly increased abundances of the vector Hyalesthes obsoletus (Hemiptera: Cixiidae) associated to the plant Urtica dioica, the reservoir of the BN pathogen stolbur tuf-type-I. The vector acquires BN-phytoplasma as larvae whilst feeding on the roots of infected U. dioica. To understand the dynamics of the Urticacycle, we tested at what instar larvae become infected and whether infection affects larvae size (i.e. growth) at two sites in the Mosel Valley, Germany. Larvae were tested from infected plants and collected at instar-stages 3, 4 and 5. Larvae at stage 3 were already infected b…
Ecology and technological capability of lactic acid bacteria isolated during Grillo grape vinification in the Marsala production area
2011
Grapes of the “Grillo” variety, used to produce Marsala wine, were harvested from five vineyards with different climatic and agronomic parameters, in order to obtain a first mapping of lactic acid bacteria (LAB) inhabiting the production area. Marsala base wine production was followed at a large-scale, and also two experimental vinifications, with different lysozyme and SO2 concentrations and in combination, were carried out at pilot-plant scale. LAB communities and conventional chemical parameters were periodically analysed. LAB were found on grapes at an average concentration of about 102 CFU g−1 which decreased during the transformation process. A total of 146 colonies were collected, bu…