Search results for "hDPSC"
showing 3 items of 3 documents
Role of p16INK4a and BMI-1 in oxidative stress-induced premature senescence in human dental pulp stem cells
2017
Human dental pulp stem cells (hDPSCs) are a source for cell therapy. Before implantation, an in vitro expansion step is necessary, with the inconvenience that hDPSCs undergo senescence following a certain number of passages, loosing their stemness properties. Long-term in vitro culture of hDPSCs at 21% (ambient oxygen tension) compared with 3–6% oxygen tension (physiological oxygen tension) caused an oxidative stress-related premature senescence, as evidenced by increased β-galactosidase activity and increased lysil oxidase expression, which is mediated by p16INK4a pathway. Furthermore, hDPSCs cultured at 21% oxygen tension underwent a downregulation of OCT4, SOX2, KLF4 and c-MYC factors, w…
Alginate-Agarose Hydrogels Improve the In Vitro Differentiation of Human Dental Pulp Stem Cells in Chondrocytes. A Histological Study
2021
[EN] Matrix-assisted autologous chondrocyte implantation (MACI) has shown promising results for cartilage repair, combining cultured chondrocytes and hydrogels, including alginate. The ability of chondrocytes for MACI is limited by different factors including donor site morbidity, dedifferentiation, limited lifespan or poor proliferation in vitro. Mesenchymal stem cells could represent an alternative for cartilage regeneration. In this study, we propose a MACI scaffold consisting of a mixed alginate-agarose hydrogel in combination with human dental pulp stem cells (hDPSCs), suitable for cartilage regeneration. Scaffolds were characterized according to their rheological properties, and their…
Efecto de la estimulación mecánica sobre células madre aplicado a la regeneración de cartílago
2020
La baja capacidad de regeneración del cartílago hialino articular ha estimulado el desarrollo de numerosas terapias, con resultados poco satisfactorios hasta la fecha. Desde hace años la ingeniería tisular ha puesto a punto diferentes procedimientos in vitro para favorecer la diferenciación condrogénica de células madre mesenquimales. Debido a los buenos resultados que se han alcanzado, algunos de los constructos obtenidos con estas técnicas han acabado implantándose en pacientes con lesiones condrales u osteocondrales, pero con fracasos a medio y largo plazo en muchos de los casos. Es por ello que esta Tesis Doctoral tiene como objetivo seguir profundizando en las técnicas de ingeniería ti…