Search results for "histologic"

Standardization of reagents and methods used in cytological and histological practice with emphasis on dyes, stains and chromogenic reagents

1994

The need for the standardization of reagents and methods used in the histology laboratory is demonstrated. After definitions of dyes, stains, and chromogenic reagents, existing standards and standards organizations are discussed. This is followed by practical instructions on how to standardize dyes and stains through the preparation of reference materials and the development of chromatographic methods. An overview is presented of the problems concerned with standardization of the Romanowsky-Giemsa stain for cytological and histological application. Finally, the problem of how to convince routine dye and stain users of the need for standardization in their histology laboratories is discussed.

Diagnostic validity of ICDAS II, VistaProof and a combination of these two methods. An in vitro study in pre-cavitated lesions

2017

Purpose In view of the present fall in advanced cavitation lesion prevalence, diagnostic methods have been developed to assist in assessing and measuring pre-cavitation lesions. The purpose of this study was to assess the VistaProof fluorescence-based system in vitro and compare the results with those of visual examination using the ICDAS II criteria. Methods 100 permanent teeth were collected and 65 were chosen at random for this study. Two separate examiners each examined them twice, 3 weeks apart, assessing them in accordance with ICDAS II. They were subsequently examined with the VistaProof system and the images were stored in the computer program. After obtaining all these data, the te…

Histophotometry: a new method for automated histological examination of solid tissue samples demonstrated on bladder cancer.

1979

The purpose of this study was to demonstrate the ability of a new method to discriminate between benign and malignant tissue. Tissue samples from three patients with bladder tumours and one patient with bladder diverticula have been examined by histophotometry. This method, based on the correlation of nuclear volume and nucleic acid contents per cell uses thick formalin fixed samples. The results are obtained by measurements averaging more than one hundred thousand cells per sample. By this histophotometric examination a clear differentiation between malignant and benign tissue could be achieved. It was also possible to determine the degree of malignancy and to differentiate between infiltr…

Improved section bonding using silanated glass slides--application protocol.

1992

Silanated slides provide excellent tissue adhesion for routine histology, immunohistochemistry and in situ hybridization of frozen, paraffin embedded material or cell smears. An easy and inexpensive method of treating glass microscope slides with triethoxysilylpropylamine is reported.

Release of dendritic cells from cognate CD4 + T-cell recognition results in impaired peripheral tolerance and fatal cytotoxic T-cell mediated autoimm…

2012

Resting dendritic cells (DCs) induce tolerance of peripheral T cells that have escaped thymic negative selection and thus contribute significantly to protection against autoimmunity. We recently showed that CD4 + Foxp3 + regulatory T cells (Tregs) are important for maintaining the steady-state phenotype of DCs and their tolerizing capacity in vivo. We now provide evidence that DC activation in the absence of Tregs is a direct consequence of missing DC–Treg interactions rather than being secondary to generalized autoimmunity in Treg-less mice. We show that DCs that lack MHC class II and thus cannot make cognate interactions with CD4 + T cells are completely unable to induce peripheral CD8 +…

The impact of tumor nitric oxide production on VEGFA expression and tumor growth in a zebrafish rat glioma xenograft model.

2015

International audience; To investigate the effect of nitric oxide on tumor development, we established a rat tumor xenograft model in zebrafish embryos. The injected tumor cells formed masses in which nitric oxide production could be detected by the use of the cell-permeant DAF-FM-DA (diaminofluorophore 4-amino-5-methylamino-2'-7'-difluorofluorescein diacetate) and DAR-4M-AM (diaminorhodamine-4M). This method revealed that nitric oxide production could be co-localized with the tumor xenograft in 46% of the embryos. In 85% of these embryos, tumors were vascularized and blood vessels were observed on day 4 post injection. Furthermore, we demonstrated by qRT-PCR that the transplanted glioma ce…

Invasive maxillary sinus aspergillosis: a case report successfully treated with voriconazole and surgical debridement

2014

Introduction: Invasive aspergillosis of the paranasal sinuses is a rare disease and often misdiagnosed; however, its incidence has seen substancial growth over the past 2 decades. Definitive diagnosis of these lesions is based on histological examination and fungal culture. Case Report: An 81-year-old woman with a history of pain in the left maxillary region is presented. The diagnosis was invasive maxillary aspergillosis in immunocompetent patient, which was successfully treated with voriconazole and surgical debridement. Possible clinical manifestations, diagnostic imaging techniques and treatment used are discussed. Since the introduction of voriconazole, there have been several reports …

Assessment of Adult Mouse Brain Neuroanatomical Phenotypes Using Quantitative and Precision Histology

2022

Modelling human neurodevelopmental disorders is important in biomedical research since the brain cannot be easily accessed in humans. In this chapter, we describe a series of standardized procedures for the reliable analysis of neuroanatomical phenotypes (NAPs) of the adult mouse brain using quantitative 2D histological practices. Our goal is to provide the reader an experimental pipeline, ranging from experimental work through to data analysis, which can be performed in any academic research setting with or without access to a histology platform. Depending on the type of sections studied, parasagittal or coronal, the assessment of brain neuroanatomy is performed at stereotaxic sections, at…

VARIANT HISTOLOGIC DIFFERENTIATION IN BLADDER CANCER TREATED WITH RADICAL CYSTECTOMY: INCIDENCE AND LONG TERM SURVIVAL IN A SINGLE INSTITUTION STUDY.

2016

Root repair after injury from mini-screw

2005

Mini-implants and mini-screws are commonly used in orthodontics to provide extra anchorage. One potential insertion site is between the roots in the alveolar process, which results in a risk of damaging the roots of neighbouring teeth. In an animal-experimental study, 20 mini-screws (bracket screw bone anchors, BSBAs) were inserted into the mandible of five beagle dogs. Each dog received two BSBAs in each lower quadrant, between the roots of the second and third, and third and fourth premolars. Sequential point labelling was performed every 6 weeks with vital stains, and apical X-rays were taken every 6 weeks. Radiographic examination demonstrated damage at three roots because of insertion …