Search results for "hprt"

showing 4 items of 4 documents

Defective dopaminergic control of contractility in colon from hypoxanthine‐guanine phosphoribosyltransferase deficient (HPRT‐) knockout mice

2009

Settore BIO/09 - FisiologiaHPRT DOPAMINE COLON
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DEFECTS IN DOPAMINERGIC PATHWAY AFFECT CONTRACTILITY OF COLON FROM HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE (HPRT) KNOCKOUT MICE.

2010

Lesch-Nyhan disease is an X-linked neurobehavioral and metabolic disorder caused by lack of hypoxanthine phosphoribosyltransferase (HPRT), a housekeeping enzyme responsible for recycling purines. The mechanisms underlying the neuropathology are not well understood, but the main neurochemical defect, in central nervous system, is linked to a dysfunction of dopaminergic pathway. In the enteric nervous system the dopaminergic nerves are a subset neurons of regulating gut motility, thus we investigated possible changes in colonic motility of HPRT ¯mice related to the dopaminergic control. We evaluated, in vitro, the mechanical activity of circular muscle strips from wild type and HPRT – mouse c…

hprt dopamine gastrointestinal tract
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The Friedreich's Ataxia protein frataxin modulates DNA base excision repair in prokaryotes and mammals

2010

DNA-repair mechanisms enable cells to maintain their genetic information by protecting it from mutations that may cause malignant growth. Recent evidence suggests that specific DNA-repair enzymes contain ISCs (iron–sulfur clusters). The nuclearencoded protein frataxin is essential for the mitochondrial biosynthesis of ISCs. Frataxin deficiency causes a neurodegenerative disorder named Friedreich's ataxia in humans. Various types of cancer occurring at young age are associated with this disease, and hence with frataxin deficiency. Mice carrying a hepatocyte-specific disruption of the frataxin gene develop multiple liver tumours for unresolved reasons. In the present study, we show that frata…

Iron-Sulfur ProteinsDNA Repairmedicine.disease_causeBiochemistryDNA Glycosylases8-oxoG 78-dihydro-8-oxoguanineMice0302 clinical medicineIron-Binding Proteinsoxidative stressBER base excision repairCells CulturedMammalsMice Knockout0303 health sciencesfrataxinDMEM Dulbecco's modified Eagle's mediumbiologyLiver NeoplasmsSalmonella entericairon–sulfur clusterLife SciencesIron-binding proteinsTransfection3. Good healthLB Luria–BertaniOGG1 8-oxoguanine DNA glycosylase 1ISC iron–sulfur clusterFpg formamido-pyrimidine DNA glycosylaseHPRT hypoxanthine phosphoribosyltransferaseResearch ArticleDNA damageDNA repairSSB DNA single-strand breakTransfectionCell Line03 medical and health sciencesFRDA Friedreich's ataxiaROS reactive oxygen speciesmedicineAnimalsHumansMUTYH human mutY homologue (Escherichia coli)Molecular BiologyGene030304 developmental biologyFriedreich's ataxiaCell BiologyFibroblastsMolecular biologytumorigenesisProkaryotic CellsFriedreich AtaxiaDNA base excision repairDNA glycosylaseMutationHepatocytesFrataxinbiology.proteinInstitut für ErnährungswissenschaftCarcinogenesisMAPK mitogen-activated protein kinase030217 neurology & neurosurgeryDNA Damage
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Changes in the contractility of colon from hypoxanthine-guanine phosphoribosyltransferase (HPRT) knockout mice (Lesch-Nyhan disease)

2009

ENTERIC NEURONS DOPAMINE HPRTSettore BIO/09 - Fisiologia
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