Search results for "human tissue"

showing 5 items of 15 documents

Intracellular signalling via the AKT axis and downstream effectors is active and prognostically significant in cancer of unknown primary (CUP): a stu…

2012

Background: Hypothesising that cancer of unknown primary (CUP) may harbour unique characteristics, we present a translational study of the immunohistochemical expression and clinical correlation of key PTEN/AKT pathway molecules. Patients and methods: We collected 100 paraffin-embedded CUP tissue blocks. We studied using tissue microarrays the expression of PTEN, phospho-AKT, Cyclin D1, p21, phospho-RPS6. From the percentage of staining tumour cells and the literature, we selected cut-offs to classify the expression of each biomolecule. We correlated IHC expression with clinical data. Results: PTEN, pAKT, and pRPS6 showed frequent expression. At univariate analysis, high IHC expression of p…

OncologyMalePathologyP21Signal transductionMitogen activated protein kinaseTissue microarrayCancer riskNeoplasmsSquamous cell carcinomaCarcinomatous peritonitisCancer of unknown primary (cup)MedicineOverall survivalPriority journalSurvival timeUnivariate analysisTissue microarraybiologyUnknown primaryHematologyClassificationPrognosisImmunohistochemistryPtenRetrospective studyOncologyIntracellular signalingImmunohistochemistryFemaleCyclin d1Cancer tissueProtein p21HumanSignal Transductionmedicine.medical_specialtyTranslational studyMajor clinical studyCancer mortalityAdenocarcinomaArticleCyclin D1Disease associationInternal medicineTissue array analysisPTENHumansHuman tissueProtein kinase BPI3K/AKT/mTOR pathwayCancer prognosisSurvival predictionDigestive system cancerbusiness.industryAkt/PKB signaling pathwayAktCancer of unknown primary siteProto-oncogene proteins c-aktRps6Protein kinase bTissue Array Analysisbiology.proteinProtein expressionProgression free survivalProtein s6Neoplasms Unknown PrimarybusinessTissue preparationProto-Oncogene Proteins c-aktAnnals of oncology : official journal of the European Society for Medical Oncology
researchProduct

Validity of needle core biopsy in the histological characterisation of mammary lesions

2006

Summary Over the last few years, there has been an enormous increase in the use of needle core biopsy (CB) for the histopathological characterisation of suspect lesions of the breast. The aim of this study was to verify the diagnostic reliability of CB by comparing the histological results obtained with the use of this technique with those obtained from the whole of the surgically resected specimen. We studied 198 out of 426 patients with clinically and/or radiologically suspect breast lesions. We found correspondence between the histological examination of the whole of the excised specimen and that of the CB in 94.9% of the cases of infiltrating carcinoma and in 71.4% of those involving du…

Pathologymedicine.medical_specialtyReproducibility of ResultBreast NeoplasmsSensitivity and SpecificityBreast cancerNeedle core biopsyRetrospective StudiemedicineCarcinomaHumansbreast carcinomahumanintermethod comparisonarticle; breast carcinoma; breast disease; carcinoma in situ; diagnostic accuracy; diagnostic value; histopathology; human; human tissue; intermethod comparison; intraductal carcinoma; major clinical study; needle biopsy; prediction; priority journal; reliability; sensitivity and specificity; validation process Biopsy Needle; Breast Neoplasms; Carcinoma Ductal Breast; Carcinoma Intraductal Noninfiltrating; Female; Humans; Reproducibility of Results; Retrospective Studies; Sensitivity and Specificity [EMTREE medical terms]Retrospective Studiesintraductal carcinomaHistological examinationreliabilitybusiness.industryBiopsy Needlevalidation process MeSH: Biopsy NeedleCarcinoma Ductal Breastbreast diseaseReproducibility of Resultscarcinoma in situneedle biopsypredictionGeneral Medicinemedicine.diseasemajor clinical studyPredictive valuehuman tissueCarcinoma Intraductal Noninfiltratingpriority journalhistopathologyEMTREE medical terms: articlediagnostic accuracyFemaleSurgerydiagnostic valueRadiologybusinessCore biopsyBreast NeoplasmThe Breast
researchProduct

iGEMS : an integrated model for identification of alternative exon usage events

2016

DNA microarrays and RNAseq are complementary methods for studying RNA molecules. Current computational methods to determine alternative exon usage (AEU) using such data require impractical visual inspection and still yield high false-positive rates. Integrated Gene and Exon Model of Splicing (iGEMS) adapts a gene-level residuals model with a gene size adjusted false discovery rate and exon-level analysis to circumvent these limitations. iGEMS was applied to two new DNA microarray datasets, including the high coverage Human Transcriptome Arrays 2.0 and performance was validated using RT-qPCR. First, AEU was studied in adipocytes treated with (n = 9) or without (n = 8) the anti-diabetes drug,…

WHITE ADIPOCYTESPHYSICAL-ACTIVITYDIFFERENTIATIONARRAY ANALYSISSPLICE VARIANTSRNA-SEQ3111 BiomedicineHUMAN TISSUESTRANSCRIPTOMEalternative exon usageMICROARRAYS3142 Public health care science environmental and occupational healthGENE-EXPRESSION
researchProduct

Histologic analysis of specimens from laparoscopic endometrioma excision performed by different surgeons: does the surgeon matter?

2011

Objective To evaluate whether the amount of ovarian tissue inadvertently removed along with the endometrioma cyst wall at laparoscopy differs in relation to the operating surgeon's level of expertise. Design Multicenter, prospective trial. Setting Four tertiary care university hospitals. Patient(s) Fifty patients, aged 25 to 40 years, with monolateral ovarian endometriomas who underwent laparoscopic excision. Intervention(s) Operation with the stripping technique by surgeons with specific expertise in endometriosis surgery in four centers (groups A, B, C, and D) and by residents with average training in laparoscopic surgery (group E). Main Outcome Measure(s) Histologic examination for the e…

endometriosisLaparoscopic surgerymedicine.medical_treatmentEndometriosisEndometriosis laparoscopy ovarian cystsGynecologic Surgical Proceduresmorphologyovarian endometriomaMedicineSingle-Blind MethodOvarian DiseasesLaparoscopyclinical articleresidency educationmedicine.diagnostic_testmedical specialistarticleObstetrics and Gynecologyendometriosis; laparoscopy; ovarian cystsOrgan Sizelaparoscopic surgeryTumor Burdenendometrium tumorfemaleuterus surgerymedicine.anatomical_structurepriority journalstripping techniquehistopathologyClinical CompetenceAdultmedicine.medical_specialtyOvaryPhysiciansHumanshumanOvarian cystbusiness.industryOvaryHistologymedicine.diseasehuman tissuethicknessSurgeryEndoscopyadult; article; clinical article; endometriosis; endometrium tumor; female; histopathology; human; human tissue; laparoscopic surgery; medical specialist; morphology; multicenter study; ovarian endometrioma; ovary; priority journal; residency education; stripping technique; surgeon; thickness; uterus surgery Adult; Clinical Competence; Endometriosis; Female; Gynecologic Surgical Procedures; Humans; Laparoscopy; Organ Size; Ovarian Diseases; Ovary; Physicians; Single-Blind Method; Tumor Burdenmulticenter studySettore MED/40 - GINECOLOGIA E OSTETRICIAReproductive MedicinesurgeonLaparoscopyHistopathologybusiness
researchProduct

TP53 mutations and S-phase fraction but not DNA-ploidy are independent prognostic indicators in laryngeal squamous cell carcinoma

2005

To prospectively evaluate the prognostic significance of TP53, H-, K-, and N-Ras mutations, DNA-ploidy and S-phase fraction (SPF) in patients affected by locally advanced laryngeal squamous cell carcinoma (LSCC). Eight-one patients (median follow-up was 71 months) who underwent resective surgery for primary operable locally advanced LSCC were analyzed. Tumor DNA was screened for mutational analysis by PCR/SSCP and sequencing. DNA-ploidy and SPF were performed by flow cytometric analyses. Thirty-six patients (44%) had, at least, a mutation in the TP53 gene. Of them, 22% (8/36) had double mutations and 3% (1/36) had triple mutations. In total, 46 TP53 mutations were observed. The majority (41…

squamous cell carcinomasingle strand conformation polymorphismPrognosipolymerase chain reactionDNA Mutational AnalysisEMTREE drug terms: protein p53 EMTREE medical terms: advanced cancerS PhaseDNA Mutational AnalysiHumansprotein p53 advanced cancer; article; cell cycle S phase; DNA content; exon; flow cytometry; follow up; gene; gene mutation; genetic analysis; histopathology; human; human tissue; larynx carcinoma; multivariate analysis; ploidy; polymerase chain reaction; priority journal; prospective study; single strand conformation polymorphism; squamous cell carcinoma; tp53 gene Carcinoma Squamous Cell; DNA Mutational Analysis; DNA Neoplasm; Genes ras; Humans; Laryngeal Neoplasms; Mutation; Ploidies; Polymorphism Single-Stranded Conformational; Prognosis; S Phase; Survival Rate; Tumor Suppressor Protein p53 [EMTREE drug terms]follow uplarynx carcinomatp53 gene MeSH: Carcinoma Squamous Cellexongene mutationhumanmultivariate analysigeneLaryngeal NeoplasmsPolymorphism Single-Stranded ConformationalLaryngeal NeoplasmPloidiesflow cytometryarticleploidyDNA NeoplasmPrognosisGenes rahuman tissueSurvival RateGenes rascell cycle S phasepriority journalDNA contentgenetic analysiMutationCarcinoma Squamous CellhistopathologyTumor Suppressor Protein p53Ploidieprospective study
researchProduct