Search results for "iGEM"
showing 10 items of 124 documents
Identification and molecular characterization of an antigen of Babesia bigemina with homology to the Apical Membrane Antigen 1 (AMA-1) of Apicomplexa
2008
Babesia bigemina Apical Membrane Antigen-1: features of the gene and predictive information of the protein from Italian strains
2010
Babesia bigemina is a tick-borne haemoparasite affecting cattle in different parts of the world. One of the few antigens characterized in this species codifies for the Apical Membrane Antigen 1 (AMA-1), a transmembrane protein recently identified. In this work, we characterized the ama-1 gene from four Italian B.bigemina strains, two obtained from Ragusa, Sicily (ITA1 and ITA3), one obtained from Benevento, Campania (ITA2) and one coming from Perugia, Umbria (ITA4). Italian sequences were compared to the Australian strain, whose sequence is available in the Sanger Institute web site, and to some strains coming from different parts of the world. This study provides useful information on the …
Sequence variability in the Babesia bigemina merozoite surface antigen GP45 gene among Mexican and Italian isolates
2008
Characterization of a novel Babesia bigemina gene and its potential use as molecular marker for strain discrimination
2008
Babesia bovis and B. bigemina diffusion in Eastern Sicily
2007
Investigation on the presence of Babesia bovis and Babesia bigemina in wild boars (Sus scrofa).
2007
Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids.
2009
This paper reports two new quantitative PCR (qPCR) assays, developed in an attempt to improve the detection of bovine piroplasmids. The first of these techniques is a duplex TaqMan assay for the simultaneous diagnosis of Babesia bovis and B. bigemina. This technique is ideal for use in South America where bovids harbour no theilerids. The second technique, which is suitable for the diagnosis of both babesiosis and theileriosis worldwide, involves fluorescence resonance energy transfer (FRET) probes. In FRET assays, Babesia bovis, B. divergens, Babesia sp. (B. major or B. bigemina), Theileria annae and Theileria sp. were all identifiable based on the melting temperatures of their amplified f…
Fixed constructions in French and Chinese
2017
Languages are not just instruments of communication. Claude Hagège (2012: 181) reminds that all languages are rooted in a pool of knowledge, sensations, memories, images, dreams, which are the fabric of a speaker's competence. Languages are very complex and multidimensional phenomena. However, it is impossible to carry out a complete study of languages, without taking into account a generalized and pervasive phenomenon: the fixedness.In light of a great deal of progress which has been made by the research on phraseology, we realize that many gray areas still exist; indeed, some true mysteries remain to be unraveled. Contrastive studies have always helped to advance knowledge of language, es…
Changing paradigm in mild traumatic brain injury research
2016
Traumatic brain injury (TBI) is a major cause of death and disability among young adults. Recent data show that TBI affects about 1.7 million people annually in the United States (Faul and Coronado, 2015). After TBI, the primary injury produces almost irreparable brain damage. However, recent experimental studies have shown evidence for dynamic brain repair following TBI because endogenous progenitor cells may play regenerative roles in response to injuries (McGinn and Povlishock, 2015). In surviving patients, what plays a critical role in the clinical prognosis is the subsequent secondary injury; without effective treat- ment, cascades that include glutamatergic excitotoxicity and calcium …