Search results for "ibi"
showing 10 items of 13473 documents
Multispecies coinfections and presence of antibiotics shape resistance and fitness costs in a pathogenic bacterium
2023
Increasing antimicrobial resistance (AMR) poses a challenge for treatment of bacterial diseases. In real life, bacterial infections are typically embedded within complex multispecies communities and influenced by the environment, which can shape costs and benefits of AMR. However, knowledge of such interactions and their implications for AMR in vivo is limited. To address this knowledge gap, we investigated fitness-related traits of a pathogenic bacterium (Flavobacterium columnare) in its fish host, capturing the effects of bacterial antibiotic resistance, coinfections between bacterial strains and metazoan parasites (fluke Diplostomum pseudospathaceum) and antibiotic exposure. We quantifie…
Assembly and Translocation of Papillomavirus Capsid Proteins
2002
ABSTRACT The major and minor capsid proteins of polyomavirus are preassembled in the cytoplasm and translocated to the nucleus only as a VP1-VP2/VP3 complex. In this study, we describe independent nuclear translocation of the L1 major protein and the L2 minor capsid protein of human papillomavirus type 33 by several approaches. First, we observed that expression and nuclear translocation of L2 in natural lesions precede expression of L1. Second, using a cell culture system for coexpression, we found that accumulation of L2 in nuclear domain 10 (ND10) subnuclear structures precedes L1 by several hours. In contrast, complexes of L2 and mutants of L1 forced to assemble in the cytoplasm are tra…
Human Papillomavirus Type 16 E7 Peptide-Directed CD8+ T Cells from Patients with Cervical Cancer Are Cross-Reactive with the Coronavirus NS2 Protein
2003
ABSTRACTHuman papillomavirus type 16 (HPV16) E6 and E7 oncoproteins are required for cellular transformation and represent candidate targets for HPV-specific and major histocompatibility complex class I-restricted CD8+-T-cell responses in patients with cervical cancer. Recent evidence suggests that cross-reactivity represents the inherent nature of the T-cell repertoire. We identified HLA-A2 binding HPV16 E7 variant peptides from human, bacterial, or viral origin which are able to drive CD8+-T-cell responses directed against wild-type HPV16 E7 amino acid 11 to 19/20 (E711-19/20) epitope YMLDLQPET(T) in vitro. CD8+T cells reacting to the HLA-A2-presented peptide from HPV16 E711-19(20)recogni…
Quantitative microscopy reveals stepwise alteration of chromatin structure during herpesvirus infection
2019
During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening arou…
Fractional Hardy inequalities and visibility of the boundary
2013
We prove fractional order Hardy inequalities on open sets under a combined fatness and visibility condition on the boundary. We demonstrate by counterexamples that fatness conditions alone are not sufficient for such Hardy inequalities to hold. In addition, we give a short exposition of various fatness conditions related to our main result, and apply fractional Hardy inequalities in connection to the boundedness of extension operators for fractional Sobolev spaces.
(A,B) In vivo GCaMP6f signals recorded in layers M1, M5 and M9/10 of Mi1 (A) and Tm3 (B) neurons, before (blue, green) and after (gray, red) applicat…
2019
Sensory systems sequentially extract increasingly complex features. ON and OFF pathways, for example, encode increases or decreases of a stimulus from a common input. This ON/OFF pathway split is thought to occur at individual synaptic connections through a sign-inverting synapse in one of the pathways. Here, we show that ON selectivity is a multisynaptic process in the Drosophila visual system. A pharmacogenetics approach demonstrates that both glutamatergic inhibition through GluClα and GABAergic inhibition through Rdl mediate ON responses. Although neurons postsynaptic to the glutamatergic ON pathway input L1 lose all responses in GluClα mutants, they are resistant to a cell-type-specifi…
Alla ricerca dell'autosostenibilità. Visioni e scenari per territorio e comunità
2021
Riflettere e ritornare a ripensare le relazioni tra le varie arti e i vari campi del sapere scientifico è oggi quanto mai necessario vista la tendenza esplicita alla liquefazione, allo scollamento e alla dispersione tra e dei processi intellettuali (sempre più omologati sulla necessità di aver successo mediatico nel messaggio immediato e mediante slogan e packaging più che risolvere, concretamente, i fatti urbani e territoriali) e alla costante separazione con le tecniche, i metodi e le attività burocratiche (anche esse sempre più meccaniche e industriali che fanno spesso il paio con processi di economie di scala). Entrambi gli aspetti divengono sempre più seriali, globalizzati e standardiz…
Long-term physical activity modifies automatic visual processing
2019
Electrophysiologically registered visual mismatch negativity (vMMN) is known to represent automatic visual processing in human visual cortex. Since physical activity (PA) is generally beneficial to cerebrovascular function, we wanted to find out if automatic visual processing is affected by PA. We investigated the connection between long-term leisure-time PA and precognitive visual processing in 32 healthy young males. Participants were divided into active (n = 16) and inactive (n = 16) group according to their leisure-time PA records from the past three years. vMMN was recorded with electroencephalogram using passive oddball paradigm with visual bars. Standard (90%) and deviant (10%) stimu…
La visualisation au service de l'analyse d'interactions Implémentation d'une forme de visualisation générique au sein d'un environnement d'analyse de…
2009
International audience; La visualisation de traces d'interactions est une composante importante de leur analyse dans le cadre de l'évaluation d'usages d'EIAH collaboratifs. Différentes présentations permettent en effet aux chercheurs de plus facilement explorer des corpus parfois très complexes. Dans cet article, nous examinons les besoins de chercheurs désirant visualiser et analyser des traces d'interaction et introduisons la notion de partition d'interaction, une forme générique de visualisation adaptée à ces besoins. Nous montrons de quelle manière cette fonction a été implémentée au sein de Tatiana, un environnement d'analyse de traces. En particulier, nous illustrons comment l'archite…
Volumes of aqueous block copolymers based on poly(propylene oxides) and poly(ethylene oxides) in a large temperature range: A quantitative description
2006
The focus of this paper was on a quantitative comprehension of temperature effect on the volumes of aqueous di-block and triblock copolymers, based on propylene oxide (PO) and ethylene oxide (EO) units. To this purpose, literature data dealing with (EO316PO94 + water) and (EO13PO30EO13 + water) mixtures were analyzed. The volume vs. temperature trends were rationalized on the basis of the (unimers + aggregate) equilibrium by taking into account the temperature effect on both the partial molar volumes of the unimeric and the aggregated copolymer as well as the equilibrium constant of micellization. The analysis extended to the expansibility allowed to quantify the contribution for the shift …