Search results for "ila"

Quantification de la dynamique sédimentaire en contexte anthropisé. L'érosion des versants viticoles de Côte d'Or.

2007

The wine slopes of Côte d'Or (Burgundy, France) are subject to the combined effects of runoff/erosion processes and intense cultivation practices. The purpose of this study is to characterize the erosion processes, and to quantify the sedimentary dynamics over different time scales and spatial scales. Sedimentary budgets show that the studied slopes, with grounds that are made very sensitive to the runoff phenomena by intensive mechanisation, are subject to erosion rates of several millimetres per year. Overflow triggering and its extension, which determine sediment transfer distances, are dependent on a runoff threshold. The existence of this threshold is observed through the analysis of t…

Error messages in relational database management systems: A comparison of effectiveness, usefulness, and user confidence

2021

Abstract The database and the database management system (DBMS) are two of the main components of any information system. Structured Query Language (SQL) is the most popular query language for retrieving data from the database, as well as for many other data management tasks. During system development and maintenance, software developers use a considerable amount of time to interpret compiler error messages. The quality of these error messages has been demonstrated to affect software development effectiveness, and correctly formulating queries and fixing them when needed is an important task for many software developers. In this study, we set out to investigate how participants ( N = 152 ) …

Understanding Estonian phraseological units on the basis of Finnish : contributing and misleading factors

2020

Vironkielistä tekstiä lukevien suomalaisten on havaittu hyödyntävän monenlaisia strategioita selvittääkseen sukukielen sanojen merkityksiä. Yksi niistä on pohjustaminen (priming). Kun kyseessä on fraseologinen yksikkö, yksi osa (prime) pohjustaa toisen osan eli kohdesanan (targetin) esiintymistä, mikäli prime on tunnistettavissa äidinkielen perusteella. Tässä artikkelissa kuvaamme muutamia erityistilanteita. Miten pohjustimen ja kohdesanan välinen etäisyys tai teemanvaihdos vaikuttavat ’petollisen ystävän’ ymmärtämiseen? Lisäksi tutkimme sitä, miten ahdasrajainen semanttinen kategoria ohjaa siitä puuttuvan jäsenen merkityksen löytämistä ja millaisia perusteita käännösratkaisuille on löydett…

Biotehnoloģisko metožu izmantošana vietējās selekcijas apšu hibrīdu pavairošanā audu kultūrā

2015

Pavairojot apšu hibrīdus audu kultūrā, nākas saskarties ar tādām problēmām kā kultivēšanas dārdzība, infekcijas un kultūras ilgtermiņa uzturēšanas izmaksas. Darba mērķis bija izstrādāt zinātnisko pamatu vietējās selekcijas apšu hibrīdu pavairošanas un uzglabāšanas efektivitātes paaugstināšanai. Darbā pārbaudīta HgCl2 virsmas sterilizācijas tehnika un tās ietekme uz mikrospraudeņu vitalitāti, kā reprezentatīvo rādītāju izmantojot hlorofila a fluorescences parametru vērtības; ex vitro pavairošanas pieeja; mikrospraudeņu uzglabāšanas iespējas +50C temperatūrā. Iegūtie rezultāti liecina, ka HgCl2 virsmas sterilizācija ir izmantojama mikrospraudeņu dezinfekcijai un augu vitalitāti būtiski neiete…

Numeerista virtauslaskentaa hila-Boltzmann-simulointimenetelmällä

2006

Microtubules and microfilaments in HSV-Infected rabbit-kidney cells.

1981

In rabbit kidney cells infected with strains of Herpes simplex virus producing either cell-rounding or polycaryocytosis. Vinblastine induced paracrystals. This could be shown by phase-contrast- and electron-microscopy. Infections were done under one-step-growth conditions or at low MOI. 90 per cent noninfected cells contained stress fibers as detected by Servablue R250-staining. Shortly after recruitment into polycaryocytes, stress fibres of normal length appearing in criss-cross arrangement can be seen in the periphery of these cells. Later they polymerize to very long fibers and finally they are partially destroyed. The time of destruction depends on the MOI employed. By using Actinomycin…

Occlusion-derived baculovirus: interaction with human cells and evaluation of the envelope protein P74 as a surface display platform.

2008

To develop complementary baculovirus-based tools for gene delivery and display technologies, the interaction of occlusion-derived baculovirus (ODV) with human cells, and the functionality of the P74 ODV envelope protein for display of the IgG-binding Z domains (ZZP74) were evaluated. The cellular binding of ODV was concentration-dependent and saturable. Only minority of the bound virions were internalized at both 37 and 4 degrees C, suggesting usage of direct membrane fusion as the entry mode. The intracellular transport of ODV was confined in vesicular structures peripheral to the plasma membrane, impeding subsequent nuclear entry and transgene expression. Transduction of ODV was not rescu…

The effect of carvacrol on enteric viruses.

2014

Carvacrol, a monoterpenic phenol, is said to have extensive antimicrobial activity in a wide range of food spoilage or pathogenic fungi, yeast and bacteria. The aim of this study was to assess its antiviral activity on norovirus surrogates, feline calicivirus (FCV), murine norovirus (MNV), and hepatitis A virus (HAV), as well as its potential in food applications. Initially, different concentrations of carvacrol (0.25, 0.5, 1%) were individually mixed with each virus at titers of ca. 6-7 log TCID50/ml and incubated 2h at 37°C. Carvacrol at 0.5% completely inactivated the two norovirus surrogates, whereas 1% concentration was required to achieve ca. 1 log reduction of HAV. In lettuce wash wa…

Binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells

1995

Infection of cells by human papillomaviruses (HPVs) associated with malignant genital lesions has not been studied because of the lack of an in vitro system and the unavailability of virions. We have now used virus-like particles (VLPs) of HPV type 33 to analyze the initial events in the interaction of the HPV capsid with cell lines. Binding of VLPs to HeLa cells was observed in biochemical assays and by immunofluorescence. VLP binding was inhibited by antisera raised against VLPs but not by monoclonal antibodies recognizing either L1 or L2 epitopes accessible on VLPs. Under saturating conditions, approximately 2 x 10(4) VLPs were bound per cell, with a dissociation constant of about 100 pM…

Lipid Binding Controls Dimerization of the Coat Protein p24 Transmembrane Helix

2019

Abstract Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligomerization of p24 monomers was suggested to be mediated and/or stabilized via interactions within the transmembrane domain, and the p24 transmembrane helix appears to selectively bind a single sphingomyelin C18:0 molecule. Furthermore, a potential cholesterol-binding sequence has also been predicted in the p24 transmembrane domain. Thus, sphingomyelin and/or cholestero…