Search results for "illumina"

showing 10 items of 153 documents

In Search of Pathogens: Transcriptome-Based Identification of Viral Sequences from the Pine Processionary Moth (Thaumetopoea pityocampa)

2015

Thaumetopoea pityocampa (pine processionary moth) is one of the most important pine pests in the forests of Mediterranean countries, Central Europe, the Middle East and North Africa. Apart from causing significant damage to pinewoods, T. pityocampa occurrence is also an issue for public and animal health, as it is responsible for dermatological reactions in humans and animals by contact with its irritating hairs. High throughput sequencing technologies have allowed the fast and cost-effective generation of genetic information of interest to understand different biological aspects of non-model organisms as well as the identification of potential pathogens. Using these technologies, we have o…

Pine processionary mothPPMCypovirusGenes ViralTurkeylcsh:QR1-502Biological pest controlZoologyMothsBiologyArticlelcsh:MicrobiologyDNA sequencingTranscriptomesTranscriptomecypovirusPhylogeneticsVirologyiflavirusAnimalsPhylogenyIllumina dye sequencingLarvaIflavirusEcologyComputational BiologyHigh-Throughput Nucleotide SequencingMolecular Sequence AnnotationRhabdovirusPinuspine processionary mothInfectious DiseasesLarvaViruses<i>Thaumatopoea pityocampa</i>Identification (biology)Thaumatopoea pityocampaIntegumentrhabdovirustranscriptomeViruses
researchProduct

Genome-wide parent-of-origin DNA methylation analysis reveals the intricacies of human imprinting and suggests a germline methylation-independent mec…

2014

Genomic imprinting is a form of epigenetic regulation that results in the expression of either the maternally or paternally inherited allele of a subset of genes (Ramowitz and Bartolomei 2011). This imprinted expression of transcripts is crucial for normal mammalian development. In humans, loss-of-imprinting of specific loci results in a number of diseases exemplified by the reciprocal growth phenotypes of the Beckwith-Wiedemann and Silver-Russell syndromes, and the behavioral disorders Angelman and Prader-Willi syndromes (Kagami et al. 2008; Buiting 2010; Choufani et al. 2010; Eggermann 2010; Kelsey 2010; Mackay and Temple 2010). In addition, aberrant imprinting also contributes to multige…

PlacentaADNGene ExpressionBiologyMethylationGenomic ImprintingPregnancyGerm cellsGeneticsmedicineHumansEpigeneticsRNA-Directed DNA MethylationAllelesEmbryonic Stem CellsGenetics (clinical)GeneticsGenome HumanResearchDNAGenomicsDNA Methylationmedicine.diseaseUniparental disomyCèl·lules germinalsGenòmicaGerm CellsDifferentially methylated regionsDNA methylationIllumina Methylation AssayCpG IslandsFemaleMetilacióGenomic imprintingReprogrammingGenome Research
researchProduct

Sialotranscriptomics of the argasid tick ornithodoros moubata along the trophogonic cycle

2021

32 páginas, 8 tablas, 6 figuras

Proteomics0301 basic medicineSwinePhysiologyRC955-962Gene ExpressionDisease VectorsProteomicsBiochemistryTranscriptomeMedical Conditions0302 clinical medicineTicksArctic medicine. Tropical medicineGene expressionMedicine and Health SciencesHuman relapsing feverGeneticsbiologyEukaryotaGenomicsProteasesBody FluidsEnzymesBloodInfectious DiseasesFemaleMetabolic PathwaysAnatomyPublic aspects of medicineRA1-1270Transcriptome analysisVitellogeninsMetabolic Networks and PathwaysResearch ArticleIxodidaeArthropoda030231 tropical medicineTickSalivary glandsArthropod Proteins03 medical and health sciencesExocrine GlandsOrnithodoros moubataArachnidaGeneticsAnimalsXenobiotic MetabolismTick ControlOrnithodorosSalivaIllumina dye sequencingIxodesAsfarviridaeImmunityOrganismsPublic Health Environmental and Occupational HealthBiology and Life SciencesComputational BiologyProteinsGenome Analysisbiology.organism_classificationInvertebratesOrnithodoros moubataPhospholipases A2Species InteractionsMetabolism030104 developmental biologyAfricaEnzymologyMetalloproteasesAfrican swine feverTranscriptomeDigestive SystemZoology
researchProduct

A novel 3D recovery method by dynamic (de)focused projection

2011

This paper presents a novel 3D recovery method based on structured light. This method unifies depth from focus (DFF) and depth from defocus (DFD) techniques with the use of a dynamic (de)focused projection. With this approach, the image acquisition system is specifically constructed to keep a whole object sharp in all of the captured images. Therefore, only the projected patterns experience different defocused deformations according to the object’s depths. When the projected patterns are out of focus, their Point Spread Function (PSF) is assumed to follow a Gaussian distribution. The final depth is computed by the analysis of the relationship between the sets of PSFs obtained from different…

Range sensor[INFO.INFO-OH] Computer Science [cs]/Other [cs.OH]FocusDepth from defocusPas de mot clé en françaisBlur estimation3D reconstructionActive illumination pattern
researchProduct

Una Praeparatio ad Missam Pontificalem miniata da Jacopo Ravaldi per l’arcivescovo Ausiàs Despuig ritrovata a Monreale. Prime considerazioni

2022

Il saggio presenta alla comunità scientifica un manoscritto miniato quattrocentesco del Tesoro della Cattedrale di Monreale già noto ad Angela Daneu Lattanzi e recentemente ritrovato, attribuendone la committenza al cardinale Ausias Despuig e la paternità al miniatore francese Jacopo Ravaldi attivo a Roma. The essay presents to the scholarly community a 15th-century illuminated manuscript from the Treasury of the Cathedral of Monreale already known to Angela Daneu Lattanzi and recently rediscovered, attributing its commission to Cardinal Ausias Despuig and its authorship to the French illuminator Jacopo Ravaldi active in Rome.

Romamanoscritti e incunaboli miniatiAusiàs DespuigSettore L-ART/01 - Storia Dell'Arte Medievaleilluminated manuscripts and incunabulaRomeJacopo RavaldiAusias DespuigMonreale
researchProduct

Next-Generation Sequencing in Clinical Practice

2019

Abstract During the past few decades, Sanger sequencing represented the “gold standard” technique. In order to better define the mutational status of several genes at the same time, next-generation sequencing methodologies have been introduced in the molecular laboratory workflow. In the era of personalized medicine, this technological improvement plays a key role in the comprehensive molecular characterization of cancer patients in order to get a “tile” target therapy. For different cancer patients, different target therapies are available and different genes serve as clinical it relevant biomarkers. This chapter reviews the principal features of these novel technologies and their applicat…

Sanger sequencingCirculating tumor dnaLiquid biopsyIon torrentbusiness.industryComputer scienceCancerDNAGold standard (test)Computational biologymedicine.diseaseGene readerTarget therapyDNA sequencingClinical Practicesymbols.namesakeWorkflowIlluminaNext-generation sequencingmedicinesymbolsRNAMutational statusPersonalized medicinebusiness
researchProduct

Technical‐economic evaluation of the effectiveness of measures applied to the artificial lighting system of a school

2021

Ensuring optimum interior lighting is a topic of great importance, as this influences not only the well-being of users but also the optimal performance of visual tasks. Lighting can be natural, but if not sufficient, it can be compensated with artificial lighting. This study highlights a methodology for designing a new lighting system that takes into account both technical and economic aspects. The method was applied to an existing school located in southern Italy, in which the electricity consumption is related to the current lighting system. The school is chosen as being representative of the construction type and layout of many local schools. In addition, the coexistence of several visua…

SchoolArchitectural engineeringTechnologyComputer scienceQH301-705.5020209 energyQC1-999LENI02 engineering and technology010501 environmental sciences01 natural sciencesDimmingIlluminancelaw.inventionlaw0202 electrical engineering electronic engineering information engineeringGeneral Materials ScienceDaylightBiology (General)InstrumentationQD1-999Lighting0105 earth and related environmental sciencesFluid Flow and Transfer ProcessesConsumption (economics)Artificial lightbusiness.industryProcess Chemistry and TechnologyTPhysicsLEDGeneral EngineeringEnergy consumptionEngineering (General). Civil engineering (General)ComfortComputer Science ApplicationsLED lampDynamic simulationEnergy consumptionChemistryEconomic evaluationDaylightElectricityTA1-2040businessDynamic simulation
researchProduct

Quantitative Multispectral Imaging Differentiates Melanoma from Seborrheic Keratosis.

2021

Melanoma is a melanocytic tumor that is responsible for the most skin cancer-related deaths. By contrast, seborrheic keratosis (SK) is a very common benign lesion with a clinical picture that may resemble melanoma. We used a multispectral imaging device to distinguish these two entities, with the use of autofluorescence imaging with 405 nm and diffuse reflectance imaging with 525 and 660 narrow-band LED illumination. We analyzed intensity descriptors of the acquired images. These included ratios of intensity values of different channels, standard deviation and minimum/maximum values of intensity of the lesions. The pattern of the lesions was also assessed with the use of particle analysis. …

Seborrheic keratosisMedicine (General)diagnosisClinical BiochemistryMultispectral image01 natural sciencesArticleautofluorescence imaging010309 optics030207 dermatology & venereal diseases03 medical and health sciencesR5-9200302 clinical medicinediffuse reflectance imaging0103 physical sciencesmelanomaMedicineLed illuminationquantitative analysisbusiness.industryMelanomaLEDBenign lesionmedicine.diseaseIntensity (physics)Autofluorescencemelanoma; seborrheic keratosis; autofluorescence imaging; LED; dermoscopy; quantitative analysis; diffuse reflectance imaging; diagnosisseborrheic keratosisImaging techniquedermoscopybusinessNuclear medicineDiagnostics (Basel, Switzerland)
researchProduct

Knowledge engineering and semantic formalism of symbolic relations. From medieval network to web 3.0

2020

A search has always been at the center of human concerns, the one of conditions and means to represent, to control well, and to communicate effectively with his environment. While technological advances and electronic gadgets such as smartphones, the Web, and online social networks currently allow addressing this concern, it has not always been the case. Our works in this thesis treat medieval documents considered as a medium of communication and as a model of the social network in the Middle Ages. These documents are called medieval illuminations. They were luxurious paintings used in that time to represent the environment and ideal world for elites such as prince, duke, etc. Those paintin…

Semantic Relation / Influencial RelationRéseau SociauxRelation Sémantique / Relation d'influence[INFO.INFO-TI] Computer Science [cs]/Image Processing [eess.IV]OntologyIngénierie des ConnaissancesEnluminure MédiévaleNumérisation du Patrimoine CulturelKnowledge EngineeringSocial NetworkCultural heritage DigitalisationOntologieMedieval Illumination
researchProduct

Metagenomic strategies to assess genetic diversity in Phytophthora species

Settore AGR/12 - Patologia VegetaleMetabarcoding Phytophthora spp. genus specific primers Sanger/cloning sequencing pyrosequencing MiSeq Illumina
researchProduct