Search results for "illumina"

Reliability of genomic variants across different next-generation sequencing platforms and bioinformatic processing pipelines

2021

Abstract Background Next Generation Sequencing (NGS) is the fundament of various studies, providing insights into questions from biology and medicine. Nevertheless, integrating data from different experimental backgrounds can introduce strong biases. In order to methodically investigate the magnitude of systematic errors in single nucleotide variant calls, we performed a cross-sectional observational study on a genomic cohort of 99 subjects each sequenced via (i) Illumina HiSeq X, (ii) Illumina HiSeq, and (iii) Complete Genomics and processed with the respective bioinformatic pipeline. We also repeated variant calling for the Illumina cohorts with GATK, which allowed us to investigate the e…

Methods to Evaluate Lighting Quality in Educational Environments

2015

Abstract The current standard for lighting of indoor work places (EN 12464-1) essentially prescribes values of photometric quantities (illuminance, Unified Glare Index, etc.); therefore it does not allow a comprehensive analysis of the luminous environment. In Italy, educational buildings do not always comply with the standard requirements for lighting. Therefore an analysis of their current state is needed and this paper illustrates two methods, developed by the authors, to carry out this investigation: the former is based on the analysis of luminance maps obtained through the HDR imaging technique whereas the latter focuses on the evaluation of non-visual effects of light.

Illumination Correction on MR Images

2006

Objective. An important artifact corrupting Magnetic Resonance Images is the rf inhomogeneity, also called bias artifact. This anomaly produces an abnormal illumination fluctuation on the image, due to variations of the device magnetic field. This artifact is particularly strong on images acquired with a device specialized on upper and lower limbs due to their coil configuration. A method based on homomorphic filtering aimed to suppress this artifact was proposed by Guillemaud. This filter has two faults: it doesnt provide an indication about the cutoff frequency (cf) and introduces another illumination artifact on the edges of the foreground. This work is an improvement to this method because i…

Study of ambient light influence for three-dimensional scanners based on structured light

2007

Ambient light in a scene can introduce errors into range data from most commercial three-dimensional range scanners, particularly scanners that are based on projected patterns and structured lighting. We study the effects of ambient light on a specific commercial scanner. We further present a method for characterizing the range accuracy as a function of ambient light distortions. After a brief review of related research, we first describe the capabilities of the scanner we used and define the experimental setup for our study. Then we present the results of the range characterization relative to ambient light. In these results, we note a systematic error source that appears to be an artifact…

Die Transilluminationstechnik

1996

The technique of light-guided intubation is based on the principle that a source of light brought into the trachea results in clearly visible and defined transcutaneous illumination, while no illumination can be observed with the light source in the oesophagus (Fig. 1-7). The Trachlight is a reintroduced instrument for this alternative intubation technique. The essential developments are: a length-adjustable stylet with a removable internal metal wire, a brighter light source, a stable handle with tight fixation of the endotracheal tube, and a time-dependent warning device to avoid extended intubations. One hundred twenty patients (Mallampati I. ASA I-III) were included in the study (conven…

Lit environments quality: A software for the analysis of luminance maps obtained with the HDR imaging technique

2013

Abstract Energy performance of lighting systems has assumed a significant role in the assessment of buildings’ consumptions and consequently lighting design is aimed to reduce installed power but at the same time to achieve an excellent lighting quality both for visual and health purposes. Lighting design is nowadays essentially based on illuminance values on visual tasks and surrounding areas, but today designers’ and researchers’ interest is focused on the analysis of luminance maps obtained through video luminance meters or with numeric simulation tools. Luminance maps availability focus the attention on a more accurate study of the light reaching the eyes (luminance), rather than on the…

Color choice behavior in cats and the effect of changes in the color of the illuminant.

1993

Computational Methods for Gene Expression Profiling Using Next-Generation Sequencing (RNA-Seq)

2014

SIGNA VETUSTA MANENT: LE MACULATURAE, IL TABULARIO, I DISEGNI DI PROGETTO RITROVATI (SECC. XII – XVIII)

2014

A Multivariate Approach to Study the Bacterial Diversity Associated to the Wooden Shelves Used for Aging Traditional Sicilian Cheeses.

2022

The present study was carried to correlate the microbial diversity of the biofilms developed on the wooden boards used for aging traditional Sicilian cheeses with cheese typology. To this end, the microbial diversity of the shelves in contact with the cheeses PDO Pecorino Siciliano, PDO Piacentinu Ennese, and TAP Caciocavallo Palermitano, during ripening, was evaluated by a multivariate statistical approach. The shelf biofilms of this study were previously analyzed for their microbial composition, but no correlation between biodiversity and cheese type was investigated. Canonical discriminant analysis confirmed a cheese typology effect on the microbial loads of the wooden shelves investigat…