Search results for "image analysis."
showing 10 items of 144 documents
Standardization of islet isolation outcome- A new automatic system to determine pancreatic islet viability
2011
Pancreatic islet transplantation is emerging as a therapeutic approach for patients affected by diabetes. This approach consists of a minimally invasive procedure replacing insulin-producing cells (pancreatic islets). The technique has been proven successful, but limitations have been identified. One of the major challenges of the procedure is the counting of the isolated pancreatic islets, which is currently jeopardized by subjectivity and inaccuracy. Determination of the accurate islet number is a crucial factor in determining the correlation between the isolation product and clinical outcome. In the proposed study, we have developed software capable of objectively evaluating islet number…
Standardization of sampling and staining methods for the morphometric evaluation of sperm heads in the Cynomolgus monkey (Macaca fascicularis) using …
2005
Automated sperm morphology analysis (ASMA) technology has improved the assessment of sperm morphology, but the results depend on the use of adequate and standardized procedures. In this study the Sperm-Class Analyzer® (SCA) ASMA system was used to assess sperm head morphometry in the Cynomolgus monkey and to evaluate the influence of sample size, intraslide variation, and the use of three staining techniques on the accuracy of image processing and sperm head morphometry. Haematoxylin is the staining technique of choice for Cynomolgus spermatozoa, as optimum contrast of sperm heads with the surrounding background allows efficient segmentation, i.e. sperm head boundary detection, making the i…
Applicazioni dell’image processing nel campo delle infrastrutture viarie. Il monitoraggio della viabilità in esercizio
2010
Nel settore delle infrastrutture di trasporto, si stanno sempre più sviluppando specifiche tecniche di rilievo automatico, ad alto rendimento, utilizzate per il monitoraggio dei parametrici caratteristici del traffico e di quelli geometrici dell’infrastruttura. Di particolare interesse per la loro semplicità, flessibilità d’uso, economia ed affidabilità sono le tecniche basate sull’image processing, che prevedono l’impiego sinergico di un sistema di monitoraggio e di elaborazione delle sequenze video registrate con strumentazioni digitali o analogiche. In questo ambito, le molteplici esperienze di ricerca già maturate hanno dimostrato la versatilità di tali procedure in numerose applicazion…
Cell biology: Refined siRNA screens.
2012
A large set of high-content RNAi screens investigating mammalian virus infection and multiple cellular activities is analysed to reveal the impact of population context on phenotypic variability and to identify indirect RNAi effects.
Modeling of intracellular transport in realistic cell geometries
2018
The transport of molecules inside cells is a complex process, the characterization of which is important to gain full understanding of cellular processes. Understanding of intracellular transport is also important for medical applications, for example when analyzing transport of medicine inside cells. The intracellular environment is very complex, and at least the most crucial parts of this complexity must be accounted for to solve transport problems in cells. In this thesis the results of studies in modeling intracellular transport are presented. The aim of the work was to model intracellular transport of proteins and viral capsids in realistic cell environments. To this end, microscopic m…
Nonlinear Disorder Mapping Through Three-Wave Mixing
2010
We implement a simple and powerful approach to characterize the domain distribution in the bulk of quadratic ferroelectric crystals via far-field second-harmonic spectroscopy. The approach is demonstrated in a lithium tantalate sample with periodic electric field poling and random mark-to-space ratio.
Optical Methods for River Monitoring: A Simulation-Based Approach to Explore Optimal Experimental Setup for LSPIV
2021
Recent advances in image-based methods for environmental monitoring are opening new frontiers for remote streamflow measurements in natural environments. Such techniques offer numerous advantages compared to traditional approaches. Despite the wide availability of cost-effective devices and software for image processing, these techniques are still rarely systematically implemented in practical applications, probably due to the lack of consistent operational protocols for both phases of images acquisition and processing. In this work, the optimal experimental setup for LSPIV based flow velocity measurements under different conditions is explored using the software PIVlab, investigating perfo…
Ventricular Fibrillation detection using time-frequency and the KNN classifier without parameter extraction
2017
[ES] Este trabajo propone la detección de FV y su discriminación de TV y otros ritmos cardiacos basándose en la representación tiempo-frecuencia del ECG y su conversión en imágen como entrada a un clasificador de vecinos más cercanos (KNN) sin necesidad de extracción de parámetros adicionales. Tres variantes de datos de entrada al clasificador son evaluados. Los resultados clasifican la señal en cuatro clases diferentes: ’Normal’ para latidos con ritmo sinusal, ’FV’ para fibrilación ventricular, ’TV’ para taquicardia ventricular y ’Otros’ para el resto de ritmos. Los resultados para detección de FV mostraron 88,27% de sensibilidad y 98,22% de especificidad para la entrada de imágen equivale…
Läßt sich das sonomorphologische Lymphknotenstaging bei Kopf-Hals-Karzinomen automatisieren?
2000
OBJECTIVES B-scan ultrasound imaging is an obligatory examination of great clinical relevance in patients with head and neck cancer. The procedure, however, is dependent on an experienced examiner and thus requires higher personal resources than other examination techniques. In addition, its subjective character leads to possible deficits in reliability and objectivity. Established sonomorphologic criteria (echo-contour, echo-density, echo-structure, boundary) should therefore be matched to the findings of computer aided sonomorphometry. METHOD 200 sonographic images of the cervical region of patients with oral carcinoma were analysed semi- and fully automatically as well as interactively b…
Predicting stiffness and strength of birch pulp:Polylactic acid composites
2016
This paper studies failure of birch pulp–polylactic acid composites. Stiffness and strength are calculated using the theory of short fibre composites and the results are compared to experimental data. The results differed from the experimental values by 0–6%. With less aligned fibres the short fibre theory is not feasible. The performance of the 40 wt% birch pulp – polylactic acid composite is predicted with X-ray microtomography based finite element modelling, and the results are compared with experiments. Stiffness results differed from experiments by 1–17% . By adding into the models a third material phase representing the interface between the fibres and the matrix, the stress–strain c…