Search results for "init"

Study of the mechanisms of oxidative stress, mitochondrial function and endoplasmic reticulum stress in obesity. Role in subclinical atherosclerosis …

2019

El aumento alarmante de la prevalencia de obesidad a nivel mundial y la enorme carga de enfermedad asociada ha generado una creciente demanda de estrategias para frenar la epidemia y / o mitigar los efectos dañinos de la obesidad sobre la salud. Para ello se requiere un compromiso firme y sinérgico por parte de todas las fuerzas sociales, no sólo para desarrollar estrategias preventivas, sino también para contribuir al conocimiento de los eventos patológicos y los mecanismos involucrados en el desarrollo de esta enfermedad. Por ello, el objetivo principal de la presente tesis doctoral es profundizar en los mecanismos subyacentes de la obesidad, especialmente en aquellos relacionados con el …

Silencing of C3G increases cardiomyocyte survival inhibition and apoptosis via regulation of p-ERK1/2 and Bax

2019

Experimental studies have shown that overexpression of Rap guanine nucleotide exchange factor 1 (C3G) plays pro‐survival and anti‐apoptotic roles through molecule phosphorylated extracellular signal‐regulated kinase1/2 (p‐ERK1/2) in cardiomyocytes. However, it is still unclear if silencing of C3G may increase cell survival inhibition and apoptosis in cardiomyocytes, and whether C3G silence induced injuries are reduced by the overexpression of C3G through regulation of p‐ERK1/2 and pro‐apoptotic molecule Bax. In this study, the rat‐derived H9C2 cardiomyocytes were infected with C3G small hairpin RNA interference recombinant lentiviruses, which silenced the endogenous C3G expression in the ca…

Engineering of charge, biotin-binding and oligomerization of avidin : new tools for avidin-biotin technology

2002

Avidin is a tetrameric glycoprotein found in chicken egg white. Due to its remarkably high affinity for biotin, avidin has widely been used in biological and biotechnical applications in life sciences. Avidin is also a highly stable protein, maintaining its functional tetrameric structure at high temperatures, extremes of pH, and in the presence of strong denaturants. In the present study we wanted to find out whether different properties of avidin can be modified with protein engineering using rational design. We used the 3D-structure of avidin together with sequence information from various avidin-like proteins to plan these changes. Besides shedding more light on the protein chemical bas…

Structure‐ and Interaction‐Based Design of Anti‐SARS‐CoV‐2 Aptamers

2022

Aptamer selection against novel infections is a complicated and time-consuming approach. Synergy can be achieved by using computational methods together with experimental procedures. This study aims to develop a reliable methodology for a rational aptamer in silico et vitro design. The new approach combines multiple steps: (1) Molecular design, based on screening in a DNA aptamer library and directed mutagenesis to fit the protein tertiary structure; (2) 3D molecular modeling of the target; (3) Molecular docking of an aptamer with the protein; (4) Molecular dynamics (MD) simulations of the complexes; (5) Quantum-mechanical (QM) evaluation of the interactions between aptamer and target with …

A Service Model for Green Open Access in Finland – Why, What and How?

2017

Presentation in the ILIDE 2017 conference in Jasná, Slovakia. Presentation focuses on describing the centralized service model of green open access and how the open access goals set by EU and the Finnish Open Science and Research Initiative can be reached.

Finnish National Guidelines for Open Science

2016

Presentation in Introduction to Open Science seminar, Jyväskylä, 14.11.2016.

The Roadmap to Finnish Open Science and Research

2015

Finland published its open science roadmap at the end of November 2014. This roadmap is based on the work of the Open Science and Research Initiative (ATT), a cross-administrative initiative established by the Ministry of Education and Culture. The goal of this initiative is to promote open science and availability of information. Exploration of recent developments of open access in the EU shows that Finland is not among the leading countries in the EU. This paper focuses on the practical action plan of this roadmap and describes how the weakest part of Finnish open science, green open access is to be lifted at international top level.

XML-dokumenttien koostaminen : havaintoja opinto-opas -projektista

2005

Mathematics and Art Connections Expressed in Artworks by South African Students

2022

In this chapter, we examine a collection of drawings, and paintings from South African students between the ages of 10 to 17, that provide fresh and original perceptions to some already known topics, but also several unexpected connections between mathematics and art. These works reference classic math-art connections such as: golden ratio, spirals, infinity, and geometric figures; they also contain several personal reflections, unique discoveries and references to ethnomathematical connections within the African cultural heritage. To introduce their pieces and themselves, students shared their own interpretations of their artworks. These commentaries make possible the identification of cog…

Atomic layer deposition of AlN from AlCl3 using NH3 and Ar/NH3 plasma

2018

The atomic layer deposition (ALD) of AlN from AlCl3 was investigated using a thermal process with NH3 and a plasma-enhanced (PE)ALD process with Ar/NH3 plasma. The growth was limited in the thermal process by the low reactivity of NH3, and impractically long pulses were required to reach saturation. Despite the plasma activation, the growth per cycle in the PEALD process was lower than that in the thermal process (0.4A ° vs 0.7A ° ). However, the plasma process resulted in a lower concentration of impurities in the films compared to the thermal process. Both the thermal and plasma processes yielded crystalline films; however, the degree of crystallinity was higher in the plasma process. The…