Search results for "inocula"
showing 10 items of 206 documents
First Report of Leaf Spot and Blight Caused by Ralstonia pickettii on Bird of Paradise Tree in Italy
2008
Bird of Paradise tree (Strelitzia alba (L. f.) Skeels) is an ornamental perennial tropical plant grown in southern Italy. In the summer of 2006 and 2007, a widespread, severe leaf disease was observed on seedlings and 1- to 2-year-old plants in two glasshouses located in eastern Sicily. Disease incidence ranged from 10 to 25%. Symptoms on the leaves consisted of dark brown-to-black stripes of varying length and found between the lateral veins. Lesions sometimes coalesced into a large area of necrotic tissue. Symptomatic tissues were ground in a drop of sterile distilled water (SDW) with a scalpel. Suspensions were streaked on King's medium B (KB), nutrient agar, and yeast extract nutrient …
Survival in the soil of the ectomycorrhizal fungus Laccaria bicolor and the effects of a mycorrhiza helper Pseudomonas fluorescens
2001
In disinfected forest nursery soils, inoculating Douglas fir (Pseudotsuga menziesii) seedlings with the ectomycorrhizal fungal strain Laccaria bicolor S238N significantly increases tree growth after outplantating. However, the success of the inoculation depends on survival of the fungal inoculum in the soil during the pre-symbiotic life of the fungus. We followed the survival of L. bicolor S238N in autoclaved nursery soil in the glasshouse, and under gnotobiotic conditions in autoclaved or γ-irradiated nursery soil. We also studied the effect of the mycorrhiza helper bacterium Pseudomonas fluorescens BBc6R8, which promotes the Douglas fir-L. bicolor S238N symbiosis, on fungal viability. In …
Metarhizium anisopliae, a potential agent for the control of grape phylloxera
2004
This investigation deals with the control effects of the insect pathogenic fungus Metarhizium anisopliae var. anisopliae on Daktulosphaira vitifoliae. In pot experiments, the soil surrounding phylloxera-infected grapes was inoculated with barley colonised with M. anisopliae. After thirty-two days, ineight of ten Metarhizium-applied pots nofresh phylloxera infections could be observed.In two of ten plants, a few fresh nodositiessingly occupied with phylloxera or phylloxeraeggs could be found. In all untreated plants,fresh nodosities with either single (two of sixplants) or multiple (four of six plants)occupation with phylloxera could be observed.M. anisopliae could be re-isolated in aconcent…
Supression of Botrytis cinerea causing grey mould disease of grape vine (Vitis vinifera) and its pectinolytic activities by a soil bacterium
1997
Abstract Botrytis cinerea was found to be pathogenic to grapevine, producing characteristic grey mould symptoms within 7 days of inoculation into vitroplants. An endospore forming bacterial strain (GI 070), Bacillus circulans, was found to be antagonist against this disease causing fungus. Under laboratory conditions the bacterial culture or its filtrate brings about complete suppression of the fungus in Petri-dishes and the grey mould symptoms on grapevine vitroplants. Pectinolytic activities (Polygalacaturonase, Pectin lyases) of Botrytis cinerea were also influenced by these bacteria. Details of the fungal parasite, its biological control and its pectinolytic activities are discussed in …
Degradation of stilbene-type phytoalexins in relation to the pathogenicity of Botrytis cinerea to grapevines
1996
The ability of eight isolates of Botrytis cinerea to degrade the stilbene phytoalexins, resveratrol and pterostilbene, was compared with their pathogenicity to grapevines. All strains which degraded resveratrol and pterostilbene were highly or moderately pathogenic to in vitro cultures of grapevines (Vitis rupestris) after inoculation with agar disks containing mycelium, while those which were unable to degrade phytoalexins were non-pathogenic. In all cases, the hydroxystilbene-degrading activity was related to the presence of laccase activity in the culture filtrates, as shown by using syringaldazine as substrate. The role of laccase-mediated degradation of phytoalexins in relation to path…
Yeast interaction on Chardonnay wine composition: Impact of strain and inoculation time.
2022
Abstract It is of great importance to understand the molecular characteristics and substantial chemical transformations due to yeast-yeast interaction. Non-targeted metabolomics was used to unravel must in fermentation composition, inoculated with non-Saccharomyces (NS) yeasts and Saccharomyces cerevisiae (S) for sequential fermentation. ultrahigh-resolution mass spectrometry was able to distinguish thousands of metabolites and provides deep insights into grape must composition allowing better understanding of the yeast-yeast interactome. The dominance of S, characterized by a metabolic richness not found with NS, is dependent on inoculation time and on the yeast species present. Co-inocula…
The effect of arbuscular mycorrhizal fungi on total plant nitrogen uptake and nitrogen recovery from soil organic material
2013
SUMMARYArbuscular mycorrhizal (AM) fungi increase nitrogen (N) uptake by their host plants, but their role in plant N capture from soil organic material is still unclear. In particular, it is not clear if AM fungi compete with the host plant for the N coming from the decomposing organic matter (OM), especially when the AM extraradical mycelium (ERM) and plant roots share the same soil volume. The goal of the present research was to study the effects of AM fungi on wheat N capture after the addition of 15N-labelled OM to soil. Durum wheat (Triticum durum) was grown under controlled conditions in a sand:soil mix and the following treatments were applied: (1) AM inoculation with Glomus mosseae…
Response to salinity stress of Rhizobium leguminosarum bv. viciae strains in the presence of different legume host plants
2011
We investigated the effect of residual salts from the previous summer’s irrigation on two non-irrigated cover crops—broad bean and common vetch—and on their rhizobial symbiontics. Before sowing, seeds were inoculated with a salt-tolerant strain and a salt-sensitive strain of Rhizobium leguminosarum biovar viciae. An increase in the electrical conductivity of the saturated-soil extract from 2.0 dS m−1 to 6.0 dS m−1 caused a severe reduction of broad bean biomass, while growth of common vetch was almost unaffected by the salinity level. Our results clearly indicate that common vetch as a cover crop may increase the availability of nitrogen in soil more than broad bean also in saline environme…
Developmental dyslexia: Visual abnormalities during ocular rotation
2005
[u]Purpose[/u]: Most of the studies dealing with visual perception disabilities in children suffering from developmental dyslexia have been done in orthophoric position. The objective of the present study is to explore the visual perception during eye movements. [u]Methods[/u]: Sixty male patients (11.8 ± 1.8 years) were examined by neuropsychologists to evaluate reading difficulties. Visual perception during ocular rotation was assessed with a Clement Clarke synoptophore with G3–G4 slides, in primary gaze, then laterally at 20, 30 and 40° in dim light. [u]Results[/u]: Three main abnormalities were found. • Isolated perception abnormalities with intermittent shading of the image, central, p…
Curso de prácticas de Optometria II : práctica 8 ficha clínica
2020
Esta publicación surge con la finalidad de servir de guía, consulta y orientación para que el estudiantado, (o las personas interesadas en el tema), puedan ir siguiendo un orden de pruebas y de pasos durante la realización de la actividad relacionada con la evaluación de la visión binocular. Nuestro objetivo general es facilitar que se consoliden las bases prácticas necesarias para la evaluación óptima de la visión binocular en cada una de las partes que la componen. En esta publicación, correspondiente a la práctica 8, se muestra como plasmar los resultados de las pruebas realizadas, durante la evaluación de la visión binocular, siguiendo un orden estructurado.