Search results for "inoculant"
showing 10 items of 13 documents
Changes in the physiological and agricultural characteristics of peat-based Bradyrhizobium japonicum inoculants after long-term storage
2000
International audience; Commercial soybean inoculants processed with sterilised peat and stored at 20 °C for 1–8 years were used as experimental materials to assess the changes in the physiological activity of Bradyrhizobium japonicum after storage. Viable counts decreased and physiological characteristics of the bacterium changed during storage, with an increase in the time taken for colony appearance on a medium without yeast extract, an increase in the lag time for nodule appearance on soybean grown in glass tubes and a decrease in survival on seeds. All the inoculants produced a significant increase in grain yield in a field experiment. The percentage of efficient cells in the field (re…
Bacterial diversity associated with poplar trees grown on a Hg-contaminated site: Community characterization and isolation of Hg-resistant plant grow…
2018
International audience; Industrial waste dumps are rarely colonized by vegetation after they have been abandoned, indicating biological infertility. Revegetation of industrial tailings dumps is thus necessary to prevent wind erosion, metal leaching and has been shown to restore soil functions and ecosystem services. However, little is known about the microbial colonization and community structure of vegetated tailings following the application of restoration technologies. In this study, we investigated the rhizosphere and phyllosphere bacterial communities of a poplar tree plantation within a phytomanagement-based restoration program of a Hg-contaminated site. We used Illumina-based sequenc…
Recurrent inoculation: a strategy for a better survival of Pseudomonas fluorescens strain in soil
2022
A growing interest is shown for microbial inoculants as a promising alternative to agrochemicals for sustainable agricultural. However, biotic and abiotic stresses are known to affect their establishment and effectiveness, leading to inconsistent performance in field conditions. At the same time, a poor survival of the strain is often observed, which constitutes a main hamper for bacterial inoculant development. We hypothesize that recurrent inoculations would improve the survival of the strain in soil. For this purpose, we tested different scenarios of inoculation of the plant growth promoting rhizobacteria Pseudomonas fluorescens in soil microcosms: a single inoculation, two inoculations …
Effect of carbon source supply and its location on competition between inoculated and established bacterial strains in sterile soil microcosm
1999
The aim of this work was to study how the location of a carbon source and of bacterial cells in soil can enhance the growth of a bacterial inoculum. Studies were performed using irradiated soil and two pairs of strains of Bradyrhizobium japonicum and Pseudomonas fluorescens. For each species, an antibiotic-resistant mutant was used as inoculant and introduced into a sterile soil pre-colonised with its parent strain. The inocula and a glycerol amendment were introduced together or separately into soil using porous microgranules or by spraying a suspension onto the bulk soil. Competition was assessed by plate counts of both inoculant and established populations. Both B. japonicum and P. fluor…
Nutrient removal by rice–wheat cropping system as influenced by crop establishment techniques and fertilization options in conjunction with microbial…
2020
AbstractNutrient uptake by the rice–wheat cropping system (RWCS) is an important indicator of soil fertility and plant nutrient status. The hypothesis of this investigation was that the rate and sources of nutrient application can differentially influence nutrient removal and soil nutrient status in different crop establishment techniques (CETs). Cropping system yield was on par in all the CETs evaluated, however, there were significant changes in soil nutrient availability and microbiological aspects. The system nitrogen (N), phosphorus (P), potassium (K) and zinc (Zn) uptake in aerobic rice system followed by zero tillage wheat (ARS-ZTW) was 15.7–17.6 kg ha−1, 0.7–0.9 kg ha−1, 7–9.8 kg ha…
Impact of inoculation with the phytostimulatory PGPR Azospirillum lipoferum CRT1 on the genetic structure of the rhizobacterial community of field-gr…
2009
International audience; The phytostimulatory PGPR Azospirillum lipoferum CRT1 was inoculated to maize seeds and the impact on the genetic structure of the rhizobacterial community in the field was determined during maize growth by Automated Ribosomal Intergenic Spacer Analysis (ARISA) of rhizosphere DNA extracts. ARISA fingerprints could differ from one plant to the next as well as from one sampling to the next. Inoculation with strain CRT1 enhanced plant-to-plant variability of the ARISA fingerprints and caused a statistically significant shift in the composition of the indigenous rhizobacterial community at the first two samplings. This is the first study on the ecological impact of Azosp…
Methods for studying root colonization by introduced beneficial bacteria
2003
e-Book; Some free-living rhizobacteria are considered as potential biocontrol and plant growth-promoting agents. Successful application of beneficial bacteria as microbial inoculants requires their presence and activity at the appropriate level, but even more, at the right time and place. Various markers are described in the literature to differentiate introduced bacteria from indigenous microflora and to visualize them. These markers are presented together with the methods currently applied to quantify bacterial densities and to characterize the distribution of introduced bacteria. The methods to quantify bacterial densities are either based on bacterial cultivation or not. Different types…
Evaluation de bactéries d’intérêt pour l’agriculture ou l’environnement (biofertilisant) ; cas des inoculants à Bradyrhizobium japonicum, bactérie fi…
2014
EA MERS CT3; Master
Identification et évaluation de bactéries d’intérêt pour l’agriculture
2012
Ce rapport de Stage est Confidentiel EA MERS CT3; Licence
Identification de bactéries d’intérêt pour l’agriculture issues d’inoculants commerciaux
2014
Rapport de Stage de 1ère année de BTS EA MERS CT3; DEUG