Search results for "insertion"
showing 10 items of 393 documents
Identification of a third secondary carrier (DcuC) for anaerobic C4-dicarboxylate transport in Escherichia coli: roles of the three Dcu carriers in u…
1996
In Escherichia coli, two carriers (DcuA and DcuB) for the transport of C4 dicarboxylates in anaerobic growth were known. Here a novel gene dcuC was identified encoding a secondary carrier (DcuC) for C4 dicarboxylates which is functional in anaerobic growth. The dcuC gene is located at min 14.1 of the E. coli map in the counterclockwise orientation. The dcuC gene combines two open reading frames found in other strains of E. coli K-12. The gene product (DcuC) is responsible for the transport of C4 dicarboxylates in DcuA-DcuB-deficient cells. The triple mutant (dcuA dcuB dcuC) is completely devoid of C4-dicarboxylate transport (exchange and uptake) during anaerobic growth, and the bacteria are…
Fitness drift of an atrazine-degrading population under atrazine selection pressure.
2008
International audience; Pseudomonas sp. ADP harbouring the atrazine catabolic plasmid ADP1 was subcultured in liquid medium containing atrazine as sole source of nitrogen. After approximately 320 generations, a new population evolved which replaced the initial population. This newly evolved population grew faster and degraded atrazine more rapidly than the initial population. Plasmid profiles and Southern blot analyses revealed that the evolved strain, unlike the ancestral strain, presented a tandem duplication of the atzB gene encoding the second enzyme of the atrazine catabolic pathway responsible for the transformation of hydroxyatrazine to N-isopropylammelide. This duplication resulted …
Experimental conditions affect the site of tetrazolium violet reduction in the electron transport chain of Lactococcus lactis
2009
The reduction of tetrazolium salts to coloured formazans is often used as an indicator of cell metabolism during microbiology studies, although the reduction mechanisms have never clearly been established in bacteria. The objective of the present study was to identify the reduction mechanisms of tetrazolium violet (TV) in Lactococcus lactis using a mutagenesis approach, under two experimental conditions generally applied in microbiology: a plate test with growing cells, and a liquid test with non-growing (resting) cells. The results showed that in both tests, TV reduction resulted from electron transfer from an intracellular donor (mainly NADH) to TV via the electron transport chain (ETC), …
Insertion of isocyanides into the 2-one-1-propyl—palladium(II) bond: A convenient synthetic route to C1-palladated 1-amino-3-one-1-butene compounds
1984
Abstract Migratory insertion of isocyanides CNR (R = p -C 6 H 4 OMe, Me) into the palladium—carbon σ bond of trans -[PdCl(CH 2 COMe)(PPh 3 ) 2 ] (I) yields the 1-amino-3-one-1-butenyl complexes trans -[PdCl{C(NHR)CHCOMe}(PPh 3 ) 2 ] (IIa, R = p -C 6 H 4 OMe; IIb, R = Me), which have been characterized by IR, 1 H and 31 P NMR spectra. The reaction of IIa with nickel acetate gives a diamagnetic bischelate complex of the type [Ni(ON) 2 ] , in which the deprotonated 1-amino-3-one-1-butenyl moiety acts as a bidentate O,N ligand.
Multiple Roles of Isocyanides in Palladium-Catalyzed Imidoylative Couplings: A Mechanistic Study
2016
International audience; Kinetic, spectroscopic and computational studies examining a palladium-catalyzed imidoylative coupling highlight the dual role of isocyanides as both substrates and ligands for this class of transformations. The synthesis of secondary amides from aryl halides and water is presented as a case study. The kinetics of the oxidative addition of ArI with RNC-ligated Pd-0 species have been studied and the resulting imidoyl complex [(ArC=NR)Pd(CNR)(2)I] (Ar=4-F-C6H4, R = tBu) has been isolated and characterized by X-ray diffraction. The unprecedented ability of this RNC-ligated imidoyl-Pd complex to undergo reductive elimination at room temperature to give the amide in the p…
Educational Pathways and Gender Differences in Labor Market Entry in France
2015
International audience
Le devenir professionnel des élèves sortis des classes de BEPA
1985
L'insertion professionnelle des diplômés en France
1984
International audience
Des caractéristiques des équipes de recherche aux pratiques de recrutement en entreprise
1999
International audience
Le professionnel hautement qualifié et flexible dans la société de la connaissance
2009
09063; Rapport financé par le Conseil Régional de Bourgogne; La question centrale, fil conducteur de ce rapport, est la suivante : le diplômé de l'enseignement supérieur est-il "le professionnel hautement qualifié et flexible dans la société de la connaissance" ? Ce rapport de fin des travaux présente les principaux résultats de la recherche concernant la valorisation des compétences des diplômés européens de l'enseignement supérieur et la flexibilité sur les marchés du travail en mobilisant trois projets. L'enquête la plus récente, qui implique quinze pays d'Europe, fait l'objet du premier chapitre qui est centré sur l'analyse comparative des situations professionnelles et des perceptions …