Search results for "interference"
showing 10 items of 573 documents
Mutant HRAS as novel target for MEK and mTOR inhibitors.
2015
HRAS is a frequently mutated oncogene in cancer. However, mutant HRAS as drug target has not been investigated so far. Here, we show that mutant HRAS hyperactivates the RAS and the mTOR pathway in various cancer cell lines including lung, bladder and esophageal cancer. HRAS mutation sensitized toward growth inhibition by the MEK inhibitors AZD6244, MEK162 and PD0325901. Further, we found that MEK inhibitors induce apoptosis in mutant HRAS cell lines but not in cell lines lacking RAS mutations. In addition, knockdown of HRAS by siRNA blocked cell growth in mutant HRAS cell lines. Inhibition of the PI3K pathway alone or in combination with MEK inhibitors did not alter signaling nor had an imp…
Development of ultrasound-assisted digestion methods for the determination of toxic element concentrations in ash samples by ICP-OES
2011
The present thesis introduces several ultrasound-assisted digestion methods developed for the determination of toxic element concentrations in ash samples by inductively coupled plasma optical emission spectrometry (ICP-OES). Ultrasound-assisted digestion is a quite new technique used for sample pre-treatment purposes, especially in difficult sample matrixes. Elements determined are As, Ba, Co, Cr, Cu, Ni, Pb, Sb, Sr, V, and Zn. Most of the elements determined exist at a trace or ultra trace level in ashes and most are potential risks in the environment or human body even at low concentrations. Most of the selected elements can be determined directly by ICP-OES, but for the determination of…
Solid phase extraction materials as a key for improving the accuracy of silver nanoparticle characterization with single-particle inductively coupled…
2022
The accurate characterization of silver nanoparticles (Ag NPs) in environmental samples is crucial for understanding the potential impact of these materials on the environment and human health. Single-particle inductively coupled plasma mass spectrometry (spICP-MS) is one of the few techniques available for quantifying NPs at environmentally relevant concentrations. However, the spICP-MS detection of Ag NPs can be interfered with coexisting dissolved analyte causing high background signals, which mask NP signals leading to a significant bias in NP characterization. In this paper, a simple sample pre-treatment procedure for efficient removal of dissolved silver in natural waters is proposed …
Effect of concurrent training on trainability performance factors in youth elite golf players
2020
Background Due to the early specialization of golf players, examining the within session sequence of training should be considered to enhance performance and prevent injury risk. The present study analyzed the effects of an 18-week concurrent training developed before or after a specific golf session in adolescence elite golfers on several performance factors. Methods Sixteen right-handed male golfers, were randomly divided into two groups: after golf specific training (AG) (n = 8, age: 16.77 ± 0.58 years) and before golf specific training (BG) (n = 8, age: 16.93 ± 0.59 years). AG and BG players followed a concurrent physical conditioning program (CT) after or before the golf specific trai…
Diacylglycerol kinase α mediatses 17-β-estradiol-induced proliferation, motility, and anchorage-independent growth of Hec-1A endometrial cancer cell …
2011
Increased levels of endogenous and/or exogenous estrogens are one of the well known risk factors of endometrial cancer. Diacylglycerol kinases (DGKs) are a family of enzymes which phosphorylate diacylglycerol (DAG) to produce phosphatidic acid (PA), thus turning off and on DAG-mediated and PA-mediated signaling pathways, respectively. DGK α activity is stimulated by growth factors and oncogenes and is required for chemotactic, proliferative, and angiogenic signaling in vitro. Herein, using either specific siRNAs or the pharmacological inhibitor R59949, we demonstrate that DGK α activity is required for 17-β-estradiol (E2)-induced proliferation, motility, and anchorage-independent growth of …
Downregulation of alpha-galactosidase A upregulates CD77: functional impact for Fabry nephropathy.
2009
Anderson-Fabry disease, an inherited deficiency in the lysosomal enzyme alpha-galactosidase A, is characterized by the progressive accumulation of globotriaosylceramide (Gb3), also known as CD77. We sought to clarify the pathogenesis of Fabry disease by establishing a cell model of this disorder. The expression of alpha-galactosidase A was transiently silenced by RNA interference in HK2 and primary human renal epithelial cells and stably silenced in HK2 cells by retroviral transfection with small hairpin RNA. All of the silenced cells had histological similarities to cells of patients with Fabry disease. The cells had reduced viability, significant accumulation of intracellular Gb3, and a m…
Studies on a measles virus variant inducing persistent infections in cultured cells
1976
Attempts were made to characterized by a plaque assay two variants of the Edmonston strain of measles virus and to obtain plaque purified virus populations. The UP non-cytocidal variant, in all the examined cell systems, mainly produced small but also large plaques; the DP cytocidal variant always large plaques. Three clones, UP-SP4, UP-LP4 and DP-LP4, were derived by plaque purfication respectively of the UP small plaque, UP large plaque and DP large plaque forming particles. The virus populations of the clones could be distinguished by some other biological and physical characters: cytopathic effect in roller tube cultures, growth potential in HeLa cells, thermal stability at 45 degrees C…
Embryonic adhesion is not affected by endometrial leptin receptor gene silencing.
2006
Objective In rodents, evidence suggests that the leptin system is mandatory for embryonic implantation. We aimed to investigate the functional relevance of the endometrial leptin receptor (OB-R) in the adhesion phase of human implantation. Design We used an in vitro model for embryonic adhesion, composed of a human endometrial cell line (HEC1-A) and B6C3F1 mouse embryos. The OB-R gene was silenced in a stable manner by RNA interference, and embryonic adhesion rates were analyzed. Setting Research laboratory at a university-affiliated center. Intervention(s) RNA interference. Main Outcome Measure(s) Embryonic adhesion in cells treated with OB-R RNAi. Result(s) The OB-R shRNA-transfected cell…
Cigarette smoke alters non-neuronal cholinergic system components inducing MUC5AC production in the H292 cell line.
2013
Abstract Cigarette smoke extract (CSE) affects the expression of Choline Acetyl-Transferase (ChAT), muscarinic acetylcholine receptors, and mucin production in bronchial epithelial cells. Mucin 5AC (MUC5AC), muscarinic acetylcholine receptor M3, ChAT expression, acetylcholine levels and acetylcholine binding were measured in a human pulmonary mucoepidermoid carcinoma cell line (H292) stimulated with CSE. We performed ChAT/RNA interference experiments in H292 cells stimulated with CSE to study the role of ChAT/acetylcholine in MUC5AC production. The effects of Hemicholinium-3 (HCh-3) (50 μM) (a potent and selective choline uptake blocker) and Tiotropium bromide (Spiriva ® ) (100 nM), alone o…
Interference of Illusory Contour Perception by a Distractor
2021
The visual system is capable of recognizing objects when object information is widely separated in space, as revealed by the Kanizsa-type illusory contours (ICs). Attentional involvement in perception of ICs is an important topic, and the present study examined whether and how the processing of ICs is interfered with by a distractor. Discrimination between thin and short deformations of an illusory circle was investigated in the absence or presence of a central dynamic patch, with difficulty of discrimination varied in three levels (easy, medium, and hard). Reaction time (RT) was significantly shorter in the absence compared to the presence of the distractor in the easy and medium condition…