Search results for "internal"

Human Papillomavirus Types 16, 18, and 31 Share Similar Endocytic Requirements for Entry

2013

ABSTRACT Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.

Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs)

2008

BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16), the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contra…

Tumor targeting of baculovirus displaying a lymphatic homing peptide.

2008

Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…

The mechanism of retro-cue effect in visual working memory : Cognitive phase separation

2020

Retro-cue effect (RCE) refers to the phenomenon that individuals can use retro-cues to improve their visual working memory (VWM) performance of target items after memory stimuli disappear. To explain the mechanism of RCE in VWM, five different hypotheses have been proposed by previous studies: the hypothesis of enhancing target representations, the hypothesis of forgetting non-target representations, the hypothesis of preventing memory degradation, the hypothesis of preventing interference from probe array and the hypothesis of cognitive phase separation. Although RCE has been repeatedly observed in previous studies, the mechanism of RCE remains unclear. In this study, we conducted three ex…

视觉工作记忆中回溯线索效应的影响因素 [The Influencing Factors of Retro-Cue Effect in Visual Working Memory]

2022

The visual working memory is a temporary storage system, which encodes, maintains and retrieves visual information. The researchers have found that adding retro-cues to the maintenance phase of memory leads to changes in participants’ memory performance, which is the retro-cue effect. The magnitude of the retro-cue effect is not set in stone, but changes with the changes of its influencing factors. However, there is no study to summarize the influencing factors of the retro-clue effect. This paper looked into the articles related to the study of the retro-cue effect and summarized the influencing factors of the retro-cue effect: memory items, maintenance interval time, interference in maint…

The impact of children’s socioemotional development on parenting styles: the moderating effect of social withdrawal

2020

This study focused on associations between children’s socioemotional development (prosocial behaviour, internalizing and externalizing problems) and parenting styles (affection, behavioural control, and psychological control), and the moderating role of children’s social withdrawal (as a temperamental characteristic) in these associations. Children’s socioemotional development (n = 314) were rated by teachers at three-time points (grades 1–3). Parents completed questionnaires measuring their parenting styles at the same three-time points. The level of social withdrawal was obtained at the end of kindergarten from teachers’ reports. Panel analysis showed that prosocial behaviour was associat…

Vanhempien työ, työn siirräntä kotiin ja lasten hyvinvointi : kohtaamisia tuulikaapissa

2013

Traces of the past: an interview study with Finnish war children who did not return to Finland after the Second World War

2017

ABSTRACTThis paper is an in-depth qualitative study based on interviews with 10 Finnish children who were evacuated to Sweden during Second World War and who did not return to Finland after the war. The interviewees were asked to tell about their lives. Nine of them were between 2 and 5 years and one was 7 years old at the time of evacuation. The aim was to study how their childhood experiences were reflected in adult memories, how they remembered or did not remember. This paper focuses on the consequences of not knowing about one’s early life and also on whether it is possible to observe signs of the Finnish mother. She did not appear explicitly but could be sensed in the tendency of the i…

Prescription opioid use and employment : A nationwide Finnish register study

2021

Background: The secular decline in labor market participation and the concurrent increase in opioid use in many developed countries have sparked a policy debate on the possible connection between these two trends. We examined whether the use of prescription opioids was connected to labor market outcomes relating to participation, employment and unemployment among the Finnish population. Methods: The working-age population (aged 19–64 years) living in Finland during the period 1995–2016 was used in the analyses (consisting of 67 903 701 person-year observations). Lagged values of prescription opioid use per capita were used as the exposure. Instrumental variables (IV) estimation method was u…

Multifactorial Assessment of Risk of Falling in 753 Post-Menopausal Women: A Multicenter Cross-Sectional Study by the Italian Group for the Study of …

2020

Giovanni Iolascon,1 Alessandro de Sire,1– 3 Dario Calafiore,1,4 Maria Grazia Benedetti,5 Carlo Cisari,2,6 Giulia Letizia Mauro,7 Silvia Migliaccio,8 Ranuccio Nuti,9 Giuseppina Resmini,10 Stefano Gonnelli,9 Antimo Moretti1 1Department of Medical and Surgical Specialties and Dentistry, University of Campania “Luigi Vanvitelli”, Naples, Italy; 2Physical and Rehabilitative Medicine, Department of Health Sciences, University of Eastern Piedmont “A. Avogadro”, Novara, Italy; 3Rehabilitation Unit, “Mons L. Novarese” Hospital, Vercelli, Moncrivello, Italy; 4Section of Neuromotor Rehabilitation, Department of Neuroscience, ASST Ca…