Search results for "ionic strength"
showing 10 items of 198 documents
DNA condensation with spermine dendrimers: interactions in solution, charge inversion, and morphology control
2011
In this report, we characterize the formation of structured condensates of 884 base pair, double stranded DNA and spin-labeled, second generation dendrons (SL-G2) that are spermine-based and cationic using continuous wave electron paramagnetic resonance spectroscopy (CW EPR) and transmission electron microscopy (TEM). The electrostatic interaction between DNA and SL-G2 in solution leads to condensation of DNA into densely packed structures. At a particular charge ratio of 2.3 (cationic charges/anionic charges), the structures appear as thick rod-like condensates of parallelly ordered, stretched DNA and SL-G2. Depending on the concentration of DNA, the charge ratio and the ionic strength, wh…
Sorption of thallium(I) ions by peat.
2013
The increasing industrial use of thallium has raised the need for removal of this highly toxic element from wastewater. Thallium is more toxic than cadmium, copper, zinc, lead and mercury and as it is easily accumulated in humans, animals and plants, it poses a threat to both the environment and human health. Peat has been used as an effective, relatively cheap and easily available sorbent to treat waters containing heavy metals. In this study, peat was characterized and used as sorbent for the removal of Tl(I) ions from aqueous solution. The effect of initial Tl(I) concentration, pH, contact time, temperature and ionic strength was studied in batch mode. The maximum sorption capacity of pe…
Species-Specific Aggregation Factor in Sponges
1978
An aggregation factor (AF) from the siliceous sponge Suberites domuncula has been isolated and purified by the following steps: Sepharose 2 B gel chromatography, sucrose gradient, Nonidet treatment, Sephadex G-100 gel chromatography and DEAE-Sephadex ion-exchange chromatography. By this procedure the AF was purified 1340-fold with a 63% yield nearly to homogeneity. The AF is originally associated with large particles, characterized by a sedimentation of 2200 S. These particles have been visualized electron microscopically; they are characterized by a filament-like shape of a length of 3400 A and a cross-sectional diameter of 230 A. The purified, low-molecular weight AF has a buoyant density…
Effect of blastfurnace slag addition to Portland cement for cationic exchange resins encapsulation
2013
In the nuclear industry, cement-based materials are extensively used to encapsulate spent ion exchange resins (IERs) before their final disposal in a repository. It is well known that the cement has to be carefully selected to prevent any deleterious expansion of the solidified waste form, but the reasons for this possible expansion are not clearly established. This work aims at filling the gap. The swelling pressure of IERs is first investigated as a function of ions exchange and ionic strength. It is shown that pressures of a few tenths of MPa can be produced by decreases in the ionic strength of the bulk solution, or by ion exchanges (2Na + instead of Ca 2+ , Na + instead of K + ). Then,…
Speciation of chitosan with low and high molecular weight carboxylates in aqueous solution
2009
Quantitative data on the speciation of chitosan (310 kDa) with low and high molecular weight carboxylates in aqueous solution are reported. The following carboxylic ligands were considered: monocarboxylate (butyrate); dicarboxylates (malonate, succinate, azelate); tricarboxylate (1,2,3-propa- netricarboxylate); tetracarboxylate (1,2,3,4-butanetetracarboxylate); polyacrylates (2.0 and 20 kDa); polymethacrylate (5.4 kDa). The investigation was performed by potentiometry at t 1/4 25 C, at low ionic strength (without addition of supporting electrolyte) and at I 1/4 0:15 mol L 1 (NaCl). For all the systems the formation of (chitosan)LHi species was found (L 1/4 carboxylic ligand; i 1/4 1 to 4 de…
Sequestration of organomettalic compounds by synthetic and naturally occuring polycarboxylate ligands. Binding of monomethylmercury(II) by polyacryli…
2007
The sequestering capacity of synthetic and naturally occurring polycarboxylate ligands towards mono- methylmercury(II) was evaluated by stability quantitative data on the interaction of CH3Hgþ with different molecular weight synthetic polyacrylates (2 and 20 kDa average M.wt) and alginate (70– 100 kDa) extracted from brown algae Macrocystis pyrifera. The influence of ionic medium was evaluated by measurements on the CH3Hgþ-polyacrylate systems in NaNO3 medium at different ionic strengths (0.10, 0.25, 0.50 and 0.75mol Lÿ1), and a Debye–Hu¨ ckel type equation was used for the dependence of complex formation constants on ionic strength. Measurements on the CH3Hgþ - alginate system were carried…
Salt-induced microheterogeneities in binary liquid mixtures
2017
The salt-induced microheterogeneity (MH) formation in binary liquid mixtures is studied by small-angle x-ray scattering (SAXS) and liquid state theory. Previous experiments have shown that this phenomenon occurs for antagonistic salts, whose cations and anions prefer different components of the solvent mixture. However, so far the precise mechanism leading to the characteristic length scale of MHs has remained unclear. Here, it is shown that MHs can be generated by the competition of short-ranged interactions and long-ranged monopole-dipole interactions. The experimental SAXS patterns can be reproduced quantitatively by fitting to the derived correlation functions without assuming any speci…
Analysis and parametric sensitivity of the behavior of overshoots in the concentration of a charged adsorbate in the adsorbed phase of charged adsorb…
2003
In this work, an analysis of the parametric sensitivity of the overshoot in the concentration of the adsorbate in the adsorbed phase, which occurs under certain conditions during an ion-exchange adsorption process, is presented and used to suggest practical implications of the concentration overshoot phenomenon on operational policies and configurations of chromatographic columns and finite bath adsorption systems. The results presented in this work demonstrate and explain how the development of an overshoot in the concentration of the adsorbate in the adsorbed phase could be enhanced or suppressed by (i) varying the diffusion coefficient, D3, of the adsorbate relative to the diffusion coef…
Kinetics and equilibrium in insulin radioimmunoassay.
2002
The kinetics of insulin reaction has been studied with its specific antibody immobilized on the inner wall of the reaction tube; the radioimmunoanalytical determination of such a substance is based on the reaction. Independent variables were labelled and unlabelled insulin concentrations, temperature, viscosity, and the medium's ionic strength. Biexponential kinetics was found to be dependent on the concentrations fitted to the models discussed in the paper. The effect of temperature shows activation parameters similar to the viscous flow energy of water, which suggests that the reaction is diffusion-controlled. The results of the viscosity analysis points at the clearly negative influence …
Nonlinear hairy layer theory of electrophoretic fingerprinting applied to consecutive layer by layer polyelectrolyte adsorption onto charged polystyr…
1997
The consecutive layer-by-layer adsorption of poly(allylamine hydrochloride) (PAH) and poly(styrenesulfonate) (PSS) on colloidal charged latex particles is investigated by measuring the electrophoretic mobility as a function of pH and ionic strength over a broad range (electrophoretic fingerprinting). Meaningful interpretation of the data required the development of a nonlinear approach to hairy particle electrophoresis including dissociation, adsorption, and association. Steric and electrostatic exclusion of mobile ions from the hairy layer has been considered. Also, the surface conductivity correction is extended to the case of charged hairy layer particles. We deposited up to three polyel…