Search results for "kantasolut"
showing 10 items of 12 documents
Diskurssianalyysi kantasolututkimusta koskevista teksteistä vuosina 2001-2013
2013
Tämän tutkielman tarkoitus on perehtyä kantasolututkimuksen bioetiikkaan ja diskurssianalyysin avulla selvittää miten kantasolututkimuksesta puhuvat tiedetaustaiset sekä uskontaustaiset henkilöt ja miten diskurssit eroavat toisistaan. Empiirisenä aineistona toimivat erinäiset tieteelliset ja uskonnolliset julkaisut (n=30). Metadiskursseina toimivat lääketieteellinen eli bioeettinen sekä uskonnolliset diskurssit. Lääketieteellisestä diskurssista paljastui myös sairauksien parantamisen-, tutkimustyön-, lakiin pohjautuvan-, taloudellisen sekä uhkien aladiskurssit kun uskonnollisessa ne olivat kritisoiva, poliittinen ja taloudellinen. Tutkimuksen metodologiset ratkaisut pohjautuivat sekä perint…
Safety and efficacy outcomes after intranasal administration of neural stem cells in cerebral palsy : a randomized phase 1/2 controlled trial
2023
Abstract Background Neural stem cells (NSCs) are believed to have the most therapeutic potential for neurological disorders because they can differentiate into various neurons and glial cells. This research evaluated the safety and efficacy of intranasal administration of NSCs in children with cerebral palsy (CP). The functional brain network (FBN) analysis based on electroencephalogram (EEG) and voxel-based morphometry (VBM) analysis based on T1-weighted images were performed to evaluate functional and structural changes in the brain. Methods A total of 25 CP patients aged 3–12 years were randomly assigned to the treatment group (n = 15), which received an intranasal infusion of NSCs loade…
Evolutionary conservation and function of the human embryonic stem cell specific miR-302/367 cluster
2015
miRNA clusters define a group of related miRNAs closely localized in the genome with an evolution that remains poorly understood. The miR-302/367 cluster represents a single polycistronic transcript that produces five precursor miRNAs. The cluster is highly expressed and essential for maintenance of human embryonic stem cells. We found the cluster to be highly conserved and present in most mammals. In primates, seed sequence and miRNA structure are conserved, but inter-precursor sequences are evolving. Insertions of new miRNAs, deletions of individual miRNAs, and a cluster duplication observed in different species suggest an actively evolving cluster. Core transcriptional machinery consisti…
Stem cell therapy in rat hind limb ischemic injury
2007
Poly(alkylidenimine) Dendrimers Functionalized with the Organometallic Moiety [Ru(η5-C5H5)(PPh3)2]+ as Promising Drugs Against Cisplatin-Resistant Ca…
2018
Here and for the first time, we show that the organometallic compound [Ru(&eta
Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels
2019
Abstract Background Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development. GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG). Methods Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM fo…
Decorin Expression and Oncosuppression in Human Embryonic Carcinomas
2019
Human embryonic stem cells in culture can transform into malignant, cancer-like cells exhibiting lesser differentiation. After transplantation, these transformed cells can form highly malignant germ cell tumors. In humans, germ cell tumors often appear at gonadal sites, like in the testis. In this study, we examined the expression of small leucine rich proteoglycans in normal and karyotypically abnormal human embryonic stem cells using a publicly available transcriptome data. We also examined the expression of the small leucine rich proteoglycans in healthy human testis and in different human testicular non-seminoma germ cell tumors using IST Online database. Furthermore, we localized the e…
Component screening for novel xeno-free medium for human embryonic stem cells
2007
Maidon proteiinifraktioiden vaikutus ihmisen luuydinperäisten mesenkymaalisten kantasolujen kuntoon ja luusoluerilaistumiseen
2012
On yleisesti tiedossa, että maito on terveellistä ja parantaa luun kestävyyttä. Tutkimusten perusteella osa maidon proteiineista lisää luuta muodostavien osteoblastien määrää ja ehkäisee luun haurastumista estämällä esiosteoklastien erilaistumista luuta hajottaviksi osteoklasteiksi. Osteoblastit erilaistuvat multipotenteista mesenkymaalisista kantasoluista. Tällöin maidon proteiineilla saattaa olla fysiologinen rooli luun kasvamisessa ja paranemisessa. Maidon proteiineilla voi olla myös terapeuttinen vaikutus osteoporoosin anabolisena tekijänä. Tutkimus oli osa Valion ja Turun yliopiston yhteistyöprojektia. Tutkimuksen tarkoituksena oli testata maidon seitsemän proteiinifraktion vaikutuksia…
Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold
2022
Human pluripotent stem cells (hPSC) derived neurons are emerging as a powerful tool for studying neurobiology, disease pathology, and modeling. Due to the lack of platforms available for housing and growing hPSC-derived neurons, a pressing need exists to tailor a brain-mimetic 3D scaffold that recapitulates tissue composition and favourably regulates neuronal network formation. Despite the progress in engineering biomimetic scaffolds, an ideal brain-mimetic scaffold is still elusive. We bioengineered a physiologically relevant 3D scaffold by integrating brain-like extracellular matrix (ECM) components and chemical cues. Culturing hPSCs-neurons in hyaluronic acid (HA) gels and HA-chondroitin…