Search results for "kuolema"

showing 10 items of 117 documents

The cytoprotective protein MANF promotes neuronal survival independently from its role as a GRP78 cofactor

2021

Mesencephalic astrocyte-derived neurotrophic factor (MANF) is an endoplasmic reticulum (ER)-stress-regulated protein exhibiting cytoprotective properties through a poorly understood mechanism in various in vitro and in vivo models of neuronal and non-neuronal damage. Although initially characterized as a secreted neurotrophic factor for midbrain dopamine neurons, MANF has recently gained more interest for its intracellular role in regulating the ER homeostasis, including serving as a cofactor of the chaperone glucose-regulated protein 78 (GRP78). We aimed for a better understanding of the neuroprotective mechanisms of MANF. Here we show for the first time that MANF promotes the survival of …

0301 basic medicineBiFC bimolecular fluorescence complementationMST microscale thermophoresisPDIA1 protein disulfide isomerase family A member 1ApoptosisNEUROTROPHIC FACTOR MANFEndoplasmic ReticulumBiochemistryprotein-protein interactionMiceBimolecular fluorescence complementationUPR unfolded protein responseENDOPLASMIC-RETICULUM STRESSMesencephalonNeurotrophic factorsInsulin-Secreting CellsProtein Interaction MappingBINDINGCOMPREHENSIVE RESOURCEATF6unfolded protein response (UPR)PDIA6 protein disulfide isomerase family A member 6PPIs protein-protein interactionsEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsNPTN neuroplastinbiologyChemistryapoptosisunfolded protein responsedopamine neurons3. Good healthCell biologyGDNF glial cell line–derived neurotrophic factorIRE1-ALPHASBD substrate-binding domainendoplasmic reticulum stressMANF mesencephalic astrocyte-derived neurotrophic factorTm tunicamycinneuroprotectionResearch ArticleProtein BindingSignal TransductionGRP78Protein Disulfide-Isomerase FamilyCell SurvivalTH tyrosine hydroxylasePrimary Cell CultureSCG superior cervical ganglionProtein Disulfide-IsomerasesIRE1 inositol-requiring enzyme 1ER-STRESSER endoplasmic reticulum03 medical and health sciencesohjelmoitunut solukuolemaC-MANF C-terminal domain of MANFCSPs chemical shift perturbationsAnimalsHumansHSP70 Heat-Shock ProteinsNerve Growth FactorsNBD nucleotide-binding domainNMR nuclear magnetic resonanceMolecular Biology030102 biochemistry & molecular biologyBIPATF6Dopaminergic NeuronsGene Expression ProfilingBinding proteinneuronal cell deathDISSOCIATIONCell BiologyNEI nucleotide exchange inhibitorEmbryo MammalianadenosiinitrifosfaattiATPhermosolutmesencephalic astrocyte-derived neurotrophic factorprotein–protein interactionPERK protein kinase RNA-like ER kinaseHEK293 Cells030104 developmental biologyGene Expression RegulationChaperone (protein)Tg thapsigarginbiology.proteinUnfolded protein responseAP-MS affinity purification mass spectrometry1182 Biochemistry cell and molecular biologyGFP-SH SH-tagged GFPendoplasmic reticulum stress (ER stress)DA dopaminemesencephalic astrocyte-derived neurotrophic factor (MANF)proteiinitNeuroplastin
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Prognostic Value of Immune Environment Analysis in Small Bowel Adenocarcinomas with Verified Mutational Landscape and Predisposing Conditions

2020

Background: Small bowel adenocarcinoma (SBA) is a rare yet insidious cancer with poor survival. The abundance of tumour-infiltrating lymphocytes is associated with improved survival, but the role of the programmed death-1/programmed death ligand-1 (PD-1/PD-L1) pathway in tumour escape is controversial. We evaluated immune cell infiltration, PD1/PD-L1 expression and their prognostic value in a series of SBAs with previously verified predisposing conditions and exome-wide somatic mutation characterization. Methods: Formalin-fixed paraffin-embedded tissue sections stained for CD3, CD8, PD-L1 and PD-1 were analysed from 94 SBAs. An immune cell score (ICS) was formed from the amount of the CD3 a…

0301 basic medicineOncologyPD-L1Cancer Researchmedicine.medical_specialtyadenokarsinoomabiomarkkeritsuolistosyövätlcsh:RC254-282ArticlePathogenesis03 medical and health sciencestumour infiltrating lymphocytesohjelmoitunut solukuolema0302 clinical medicineGermline mutationImmune systemPD-L1Internal medicinesmall bowelPD-1medicineStage (cooking)adenocarcinomabiologybusiness.industryCancerlcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogensmedicine.disease3. Good health030104 developmental biologyOncologyimmuunijärjestelmä030220 oncology & carcinogenesisbiology.proteinAdenocarcinomaohutsuolisyöpätauditproteiinitlymfosyytitbusinessCD8
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Design and protocol of Estrogenic Regulation of Muscle Apoptosis (ERMA) study with 47 to 55-year-old women's cohort : novel results show menopause-re…

2018

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0301 basic medicineOncologyestradiolivaihdevuodetNeutrophilsBlood count0302 clinical medicineSurveys and QuestionnairesFSHLongitudinal Studiesmenopausal status2. Zero hungerEstradiolvalkosolutApplied MathematicsObstetrics and Gynecologyta3141ta3142Middle AgedMenstruation3. Good health17β-EstradiolMenopauseCohortComputingMethodologies_DOCUMENTANDTEXTPROCESSINGFemaleMenopauselihaskuntoestrogeenitmedicine.medical_specialtyGeneral MathematicsAffect (psychology)Statistics Nonparametric03 medical and health sciencesohjelmoitunut solukuolema17b-Estradiolneutrophil-to-lymphocyte ratioInternal medicinemedicineHumansLymphocyte CountAnalysis of VarianceChi-Square Distributionbusiness.industryOriginal Articlesleucocyte countmedicine.diseaseCross-Sectional Studies030104 developmental biologyApoptosisMultivariate AnalysisLinear Modelsblood viscosityFollicle Stimulating Hormonebusiness030217 neurology & neurosurgeryFollow-Up StudiesHormoneMenopause: The Journal of The North American Menopause Society
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PD-1 and PD-L1 expression in pulmonary carcinoid tumors and their association to tumor spread

2019

Pulmonary carcinoid (PC) tumors are rare tumors that account for approximately 1% of all lung cancers. The primary treatment option is surgery, while there is no standard treatment for metastatic disease. As the number of PCs diagnosed yearly is increasing, there is a need to establish novel therapeutic options. This study aimed to investigate programmed death protein 1 (PD-1) and programmed death ligand 1 (PD-L1) expression in PC tumors since blocking of the PD-1/PD-L1 pathway is a promising therapeutic option in various other malignancies. A total of 168 PC patients treated between 1990 and 2013 were collected from the Finnish biobanks. After re-evaluation of the tumors, 131 (78%) were cl…

0301 basic medicinePD-L1kasvaimetEndocrinology Diabetes and MetabolismCarcinoid tumorsNeuroendocrine tumorslcsh:Diseases of the endocrine glands. Clinical endocrinologyMetastasisimmunohistokemia03 medical and health sciencesohjelmoitunut solukuolema0302 clinical medicineEndocrinologyPD-L1PD-1Internal MedicineMedicinegeeniekspressiolcsh:RC648-665biologybusiness.industryStandard treatmentResearchNIVOLUMABpulmonary carcinoid tumor3126 Surgery anesthesiology intensive care radiologymedicine.diseasePrimary tumor3. Good health030104 developmental biology3121 General medicine internal medicine and other clinical medicine030220 oncology & carcinogenesisimmunohistochemistrybiology.proteinCancer researchImmunohistochemistryNivolumabbusinessNEUROENDOCRINE TUMORSneuroendocrine tumor
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Parvovirus B19V Nonstructural Protein NS1 Induces Double-Stranded Deoxyribonucleic Acid Autoantibodies and End-Organ Damage in Nonautoimmune Mice

2018

Abstract Background Viral infection is implicated in development of autoimmunity. Parvovirus B19 (B19V) nonstructural protein, NS1, a helicase, covalently modifies self double-stranded deoxyribonucleic acid (dsDNA) and induces apoptosis. This study tested whether resulting apoptotic bodies (ApoBods) containing virally modified dsDNA could induce autoimmunity in an animal model. Methods BALB/c mice were inoculated with (1) pristane-induced, (2) B19V NS1-induced, or (3) staurosporine-induced ApoBods. Serum was tested for dsDNA autoantibodies by Crithidia luciliae staining and enzyme-linked immunosorbent assay. Brain, heart, liver, and kidney pathology was examined. Deposition of self-antigens…

0301 basic medicinePathogenesis and Host ResponseviruksetvirusesB19VKidney GlomerulusSLEApoptosisAutoimmunityanti-dsDNA antibodyViral Nonstructural Proteinsmedicine.disease_causeAutoimmunityautoimmuniteettiMice0302 clinical medicineGlomerulonephritisParvovirus B19 HumanImmunology and Allergy030212 general & internal medicineEnzyme InhibitorstolerancebiologyChemistryapoptosisBrainInfectious DiseasesLivervirustauditAntibodies AntinuclearmaksatulehdusFemaleAntibodyImmunosuppressive Agentsta3111infektiot03 medical and health sciencesohjelmoitunut solukuolemaMajor Articles and Brief ReportsExtracellular VesiclesAntigenmedicineCrithidia luciliaeAnimalsapoptotic bodiesparvoviruksetParvovirusTerpenesAnti-dsDNA antibodiesMyocardiumta1183parvovirusAutoantibodyta1182DNAbiology.organism_classificationStaurosporineMolecular biology030104 developmental biologyApoptosisbiology.proteinautovasta-aineetglomerulonephritisThe Journal of Infectious Diseases
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Antiviral Properties of Chemical Inhibitors of Cellular Anti-Apoptotic Bcl-2 Proteins

2017

Viral diseases remain serious threats to public health because of the shortage of effective means of control. To combat the surge of viral diseases, new treatments are urgently needed. Here we show that small-molecules, which inhibit cellular anti-apoptotic Bcl-2 proteins (Bcl-2i), induced the premature death of cells infected with different RNA or DNA viruses, whereas, at the same concentrations, no toxicity was observed in mock-infected cells. Moreover, these compounds limited viral replication and spread. Surprisingly, Bcl-2i also induced the premature apoptosis of cells transfected with viral RNA or plasmid DNA but not of mock-transfected cells. These results suggest that Bcl-2i sensiti…

0301 basic medicinevirusesFAMILY INHIBITORSlcsh:QR1-502Virus Replicationlcsh:Microbiologychemistry.chemical_compoundTranscription (biology)SALIPHENYLHALAMIDEhost responseTRANSCRIPTIONprogrammed cell deathinnate immunity1183 Plant biology microbiology virologySulfonamidesAniline CompoundsapoptosisTransfection3. Good healthInfectious DiseasesProto-Oncogene Proteins c-bcl-2X-L INHIBITORVirus DiseasesvirustauditVirusesRNA ViralBiologyTransfectionta3111Antiviral AgentsArticleCell LineMicrobiology in the medical areaantiviral agent03 medical and health sciencesohjelmoitunut solukuolemaVirologyMikrobiologi inom det medicinska områdetHumansMetabolomicsBenzothiazolesInnate immune systemapoptosis; antiviral agent; innate immunity; host responseZIKA VIRUS-INFECTIONCHRONIC LYMPHOCYTIC-LEUKEMIAPOTENTta1183INFLUENZA-Ata1182RNAIsoquinolinesVirology030104 developmental biologyViral replicationchemistryCell cultureApoptosisCELLSREPLICATIONDNA Viral3111 BiomedicineDNA
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Coffins in Finland : the history of production, design and attitudes

2017

AbstractCoffins as death-related objects have changed in Finland during the past 150 years and the Finnish funeral industry has been created to answer the changing needs of customers. No longer do people build coffins in the household, or only buy a coffin and some other items from the funeral company: now professionals manage entire funerals. Coffin designs have become simpler and less socially discriminating and the colour formerly reserved for children and young people, white, has become the most popular colour for a coffin. Attitudes towards coffins have also changed, from mild dislike of having a coffin in the home to general demand of hiding coffins even in funeral companies’ premises…

050103 clinical psychologyHealth (social science)White (horse)Historyhistory of death05 social sciencesReligious studieskuolemacoffins03 medical and health sciencesPhilosophyAgrarian society030502 gerontologykulttuuriFinnish culture of deathSuomiEconomic historyProduction (economics)hautajaiset0501 psychology and cognitive sciencesCoffinta6150305 other medical sciencemodernisation of deathfuneralsMortality
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Alkoholinkäytön aiheuttama liikennekuoleman riski Suomessa

2023

Tässä tutkimuksessa analysoidaan juopumuksen asteen vaikutusta kuolemaan johtavan liikenneonnettomuuden riskiin moderneilla tilastollisilla menetelmillä. Tutkimuksen pääaineistona ovat vuosina 2005–2014 Suomessa tapahtuneet kuolemaan johtaneet moottoriajoneuvo-onnettomuudet, joissa oli mukana henkilöauto aiheuttajana tai muuna osallisena. Pääaineistoa täydennetään ratsiatutkimuksista julkaistuilla tilastoilla. Keskeisiä käytettyjä mittareita ovat riskisuhde sekä vakioitu riskisuhde, josta on poistettu sekoittavien muuttujien vaikutusta. Tutkimuksen ensimmäisenä tavoitteena on selvittää, miten pieni veren alkoholipitoisuus alkaa lisätä liikenneonnettomuuden riskiä, ja toisena tavoitteena on …

3142 Kansanterveystiede ympäristö ja työterveys112 Tilastotiedeliikennejuopumusliikenneturvallisuusliikenneonnettomuudetalkoholinkäyttöalkoholi (päihteet)riskitliikennekuolemat
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Effect of Cardiorespiratory Fitness on Risk of Sudden Cardiac Death in Overweight/Obese Men Aged 42 to 60 Years

2018

The purpose of this study was to examine the subject and combined associations of cardiorespiratory fitness (fitness) and body mass index (BMI) with the risk of sudden cardiac death (SCD) in middle-aged men. This prospective study was based on a population sample of 2,357 men aged 42 to 60 years, who were followed up in the Kuopio Ischemic Heart Disease cohort study. Fitness was directly measured by peak oxygen uptake (VO2peak) during progressive exercise testing to volitional fatigue. Participants were divided into 4 groups (fit-normal weight, unfit-normal weight, fit-overweight/obese, and unfit-overweight/obese) based on the median values of fitness and BMI. A total of 253 (10.7%) SCDs oc…

AdultMaleRiskmedicine.medical_specialtyobesityPopulationsudden deathheart failurecardiac arrest030204 cardiovascular system & hematologyOverweightLower risksydämen vajaatoimintasudden cardiac deathBody Mass Index03 medical and health sciences0302 clinical medicineSurveys and QuestionnairesInternal medicineFitnessHumansMedicineProspective Studies030212 general & internal medicineäkkikuolemaeducationFinlandeducation.field_of_studycardiorespiratory fitnessbusiness.industryHazard ratioVO2 maxCardiorespiratory fitnessta3141ta3142Middle AgedOverweightfatnessDeath Sudden CardiacExercise TestCardiologylihavuussydämenpysähdysmedicine.symptomCardiology and Cardiovascular MedicinebusinessBody mass indexCohort studyAmerican Journal of Cardiology
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Marriage Dissatisfaction and the Risk of Sudden Cardiac Death Among Men

2018

Conflicts in marriage have been associated with potential risk of cardiovascular disease; however, there is lack of prospective evidence on the association between marriage satisfaction and sudden cardiac death (SCD). We aimed to assess the association between perceived level of marriage satisfaction and risk of SCD. This study employed the Kuopio Ischemic Heart Disease study, an ongoing prospective population-based study in Finland. Perceived level of marriage satisfaction was assessed in 2,262 men using a well-structured self-administered questionnaire. Multivariable adjusted Cox regression models were used to estimate hazard ratios (95% confidence interval [CI]) for SCD. During a median …

AdultMalemedicine.medical_specialtyPopulation050109 social psychologyavioliittoPersonal Satisfaction030204 cardiovascular system & hematologysudden cardiac deathSudden cardiac deathmarriage satisfaction03 medical and health sciencesmarital distress0302 clinical medicineRisk Factorscardiovascular diseaseInternal medicineEpidemiologymedicineHumans0501 psychology and cognitive sciencesäkkikuolemaMarriageeducationSocioeconomic statusFinlandeducation.field_of_studybusiness.industryProportional hazards model05 social sciencesHazard ratioMenta3141ta3142Middle Agedmedicine.diseaseConfidence intervalDeath Sudden CardiactyytyväisyysCardiologysydän- ja verisuonitauditFemaleCardiology and Cardiovascular MedicinebusinessRisk assessmentDemographyAmerican Journal of Cardiology
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