Search results for "label"
showing 10 items of 797 documents
Putative p24 complexes in Arabidopsis contain members of the delta and beta subfamilies and cycle in the early secretory pathway
2013
p24 proteins are a family of type I membrane proteins localized to compartments of the early secretory pathway and to coat protein I (COPI)- and COPII-coated vesicles. They can be classified, by sequence homology, into four subfamilies, named p24α, p24β, p24γ, and p24δ. In contrast to animals and fungi, plants contain only members of the p24β and p24δ subfamilies, the latter probably including two different subclasses. It has previously been shown that transiently expressed red fluorescent protein (RFP)-p24δ5 (p24δ1 subclass) localizes to the endoplasmic reticulum (ER) at steady state as a consequence of highly efficient COPI-based recycling from the Golgi apparatus. It is now shown that tr…
Glutamine synthetase isozymes in germinating barley seeds
1993
Glutamine synthetase (GS; EC 6.3.1.2) is a key enzyme of ammonia assimilation in higher plants. In the present study the subunit composition and localization of GS in germinating barley (Hordeum vulgare) seed have been clarified. Analysis of the GS polypeptide composition by immunoblotting revealed two different polypeptides. A and B, with a molecular mass of 42 and 40 kDa, respectively. In the scutellum subunit A was already present in the ungerminated seed and remained unchanged, whereas subunit B appeared on day 2 and increased about 5-fold during germination. Polypeptide B also appeared later during germination in the aleurone layer, roots and weakly in the etiolated shoots. By immunogo…
Photoaffinity labeling of Torpedo acetylcholine receptor by physostigmine.
1993
The plant alkaloid physostigmine, an established anti-cholinesterase agent of the carbamate type, has recently been shown to bind to the nicotinic acetylcholine receptor from Torpedo marmorata electrocytes [Okonjo, K. O., Kuhlmann, J.Maelicke, A. (1991) Eur. J. Biochem. 200, 671-677]. Pharmacological studies of physostigmine-induced ion flux into nicotinic-acetylcholine-receptor-rich membrane vesicles, indicated distinct binding sites for physostigmine and acetylcholine. As shown in this study by photoaffinity labeling with [phenyl-(n)-3H](-)physostigmine, the physostigmine-binding site is located within the same subunit (alpha polypeptide) of the receptor as the acetylcholine-binding site.…
Subcellular distribution of choline acetyltransferase by immunogold electron microscopy in non-neuronal cells: Placenta, airways and murine embryonic…
2012
Abstract Aims Acetylcholine is synthesized in more or less all mammalian cells. However, little is known about the subcellular location of acetylcholine synthesis. Therefore, in the present experiments the subcellular location of the synthesizing enzyme choline acetyltransferase (ChAT) was investigated by anti-ChAT immunogold electron microscopy in human placenta and airways as well as in a murine embryonic stem cell line (CGR8 cell line). Main methods Human tissue was obtained as so-called surplus tissue (after delivery/surgical removal because of lung tumor); the CGR8 stem cell line was cultured under standard conditions. For human tissue a monoclonal mouse anti-ChAT antibody (ab) was use…
Localization of antigens PwA33 and La on lampbrush chromosomes and on nucleoplasmic structures in the oocyte of the urodele Pleurodeles waltl: Light …
1994
Monoclonal antibodies A33/22 and La11G7 have been used to study the distribution of the corre-sponding antigens, PwA33 and La, on the lampbrush chromosome loops and nucleoplasmic structures of P. waltl oocytes, using immunofluorescence, confocal laser scanning microscopy and immunogold labeling. The results obtained with these antibodies have been compared with those obtained with the Sm-antigen-specific monoclonal antibody Y12. All these monoclonal antibodies (mAbs) labeled the matrices of the majority of normal loops along their whole length. Nucleoplasmic RNP granules showed a strong staining with the mAbs La11G7 and Y12 throughout their mass, but with the mAb A33/22, they showed only a …
The Innervation of Taste Buds in the Soft Palate of the Rat as Revealed by Methylene Blue Staining.
1996
Light microscopic data on the innervation of taste buds in the soft palate of the rat obtained by methylene blue (MB) supravital staining are presented here for the first time. The taste pore including subpopulations of taste bud cells and nerve fibers were stained. Intragemmal nerve fibers were detected ascending up to the taste pore; this suggests the possibility of additional paracellular pathways in taste transduction directly via nerve fibers. Moreover, relatively large bulbous or laminar enlargements of the intragemmal nerve fibers were found. The branches of these nerve fibers, showing minute spiny protuberances, appeared to anastomose. Some perigemmal nerve fibers were also seen in …
Energy and environmental analysis of marble productive sites: «by phases» and «by single process» combined approach
2018
Energy Procedia 00 (20 1 8 ) 000 – 000 73rd Conference of the Italian Thermal Machines Engineering Association, ATI 2018, Pisa, Italy, 12 Sep 2018 - 14 Sep 2018; Italy : Elsevier Ltd. (2018).
A Generalization of Girod's Bidirectional Decoding Method to Codes with a Finite Deciphering Delay
2012
Girod’s encoding method has been introduced in order to efficiently decode from both directions messages encoded by using finite prefix codes. In the present paper, we generalize this method to finite codes with a finite deciphering delay. In particular, we show that our decoding algorithm can be realized by a deterministic finite transducer. We also investigate some properties of the underlying unlabeled graph.
Effects of a French remedial program on pupils’ educational outcomes
2018
International audience; Few studies have examined the French “Networks for Specialized Assistance to Pupils in Difficulty” (Rased). In this article, we evaluate the impact of receiving Rased services in the first year of primary schooling on academic success. Using data from a national panel study, we find inconsistency in student selection within Rased. Using matching methods, our results reveal that pupils who benefited from the program school have a higher probability of grade repetition and obtain significantly lower scores on the third grade national assessment, particularly in mathematics, compared to pupils with similar characteristics who did not take part in the program. This impac…
Do Men and Women Differ When Purchasing Private Label Goods?
2014
In the context of shopping goods, this paper analyzes the key variables in men and women when they have to face private label purchase decision: attitude towards private label, value consciousness, brand consciousness, involvement with the product and private label purchase intention. The moderating role of gender in the relationship between those variables is also analyzed. On a sample of 433 individuals, and considering two different shopping goods, results showed differences between men and women in all the variables, except in the value consciousness. Furthermore, due to a multigroup analysis technique, we found more differences between the variables affecting PL purchase intention. Acc…