Search results for "laki"

showing 10 items of 222 documents

New Acyclic Cytotoxic Jasplakinolide Derivative from the Marine Sponge Jaspis splendens

2019

A new acylic jasplakinolide congener (2), another acyclic derivative requiring revision (4), together with two jasplakinolide derivatives including the parent compound jasplakinolide (1) were isolated from the Indonesian marine sponge Jaspis splendens. The chemical structures of the new and known compounds were unambiguously elucidated based on HRESIMS and exhaustive 1D and 2D NMR spectral analysis as well as a comparison of their NMR data with those of jasplakinolide (1). The isolated jasplakinolides inhibited the growth of mouse lymphoma (L5178Y) cells in vitro with IC50 values in the low micromolar to nanomolar range.

0106 biological sciencesStereochemistryPharmaceutical Science01 natural sciencesjasplakinolide Z<sub>5</sub>Drug Discovery<i>Jaspis splendens</i>Ic50 valuesCytotoxic T cellSpectral analysisPharmacology Toxicology and Pharmaceutics (miscellaneous)lcsh:QH301-705.5cytotoxic activitybiology010405 organic chemistryChemistry010604 marine biology & hydrobiologyMouse LymphomaJaspis splendensbiology.organism_classificationIn vitro0104 chemical sciencesSpongelcsh:Biology (General)Two-dimensional nuclear magnetic resonance spectroscopyjasplakinolide Z<sub>6</sub>Marine Drugs
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Expression of NF-κB and IL-6 in oral precancerous and cancerous lesions: An immunohistochemical study.

2014

Background The purpose of this study was to evaluate the immunohistochemical expression of NF-κB and IL-6 in oral premalignant and malignant lesions and to investigate their possible correlation with the presence of subepithelial inflammation. Material and Methods Thirty two oral premalignant lesions, clinically compatible with leukoplakia or erythroplakia, were investigated. Microscopically, 11 of them showed hyperkeratosis and acanthosis (epithelial hyperplasia) and 21 showed dysplasia of varying degrees. Nine cases of OSCC and four control cases of normal oral mucosa were also included in the study. Immunohistochemical staining with NF-κB (p65) and IL-6 was performed. IL-6 and nuclear NF…

0301 basic medicineAdultMalePathologymedicine.medical_specialtyHyperkeratosisOdontologíaAcanthosis03 medical and health sciences0302 clinical medicineMedicineHumansGeneral DentistryLeukoplakiaAgedMouth neoplasmAged 80 and overErythroplakiaOral Medicine and Pathologybusiness.industryInterleukin-6ResearchNF-kappa BMiddle Aged:CIENCIAS MÉDICAS [UNESCO]medicine.diseaseCiencias de la saludImmunohistochemistrystomatognathic diseases030104 developmental biologyOtorhinolaryngologyDysplasia030220 oncology & carcinogenesisUNESCO::CIENCIAS MÉDICASImmunohistochemistrySurgeryFemaleMouth NeoplasmsbusinessPrecancerous ConditionsImmunostainingLeukoplakiaMedicina oral, patologia oral y cirugia bucal
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Effect of intermittent shear stress on corneal epithelial cells using an in vitro flow culture model.

2018

Abstract Purpose The aim of this study was to establish and to evaluate an in vitro model for culturing human telomerase-immortalized corneal epithelial (hTCEpi) cells under adjustable medium flow mimicking the movements of the tear film on the ocular surface. Methods Using an IBIDI pump system, cells were cultured under unidirectional, continuous or oscillating, discontinuous medium flow. Cell surface and cytoskeletal architecture were investigated by scanning electron microscopy and immunofluorescence. Gene expression of e-cadherin, occludin, tight junction protein (TJP), desmoplakin, desmocollin and mucins was investigated by real-time PCR. Protein expression of desmoplakin, TJP, occludi…

0301 basic medicineStress fiberTight junctionbiologyBlinkingDesmoplakinChemistryEpithelium CornealMucinsEpithelial CellsOccludinCell junctionModels BiologicalCell biology03 medical and health sciencesOphthalmology030104 developmental biologyCell culturebiology.proteinShear stressHumansStress MechanicalMUC1BiomarkersCells CulturedThe ocular surface
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Oral cancer on the gingiva in patients with proliferative leukoplakia: A study of 30 cases

2019

Background Proliferative verrucous leukoplakia, recently coined as proliferative leukoplakia (PL), is associated with a strong tendency to recur after treatment and an elevated rate of malignant transformation. We compared the clinical characteristics of patients with gingival PL with and without progression to oral squamous cell carcinoma. Methods The patients were divided into two groups: group 1 included 33 patients with gingival PL that did not progress to cancer, and group 2 included 30 patients with PL who developed malignant transformation during follow-up. We compared age, sex, tobacco habit, clinical characteristics of gingival PL lesions, and location, tumor-node-metastasis (TNM) …

0301 basic medicinemedicine.medical_specialtyGingivaMalignancyGastroenterologyMalignant transformationLesion03 medical and health sciences0302 clinical medicineTongueInternal medicinemedicineHumansIn patientStage (cooking)LeukoplakiaGingival Neoplasmsbusiness.industryCancer030206 dentistrymedicine.diseasestomatognathic diseases030104 developmental biologymedicine.anatomical_structureCarcinoma Squamous CellPeriodonticsFemaleMouth NeoplasmsLeukoplakia Oralmedicine.symptombusinessJournal of Periodontology
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Huligaaneja ja pamppukaartilaisia : kieltolain kiertäminen ja kontrollointi Saarijärvellä vuosina 1927-1930

2012

1930-lukuSaarijärvimaaseutukieltolakialkoholi1920-lukusosiaalinen kontrollikieltolait
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"Katajaisen kansan hullut huhut - kansa katu- ja kyläojissa" : Helsingin Sanomien, Raittiussanomien ja Alkoholipolitiikka- aikakauskirjan kirjoittelu…

2012

Tutkin työssäni vuonna 1968 päätetyn alkoholilain ja erillisen keskiolutlain ympärillä käytyä julkista keskustelua. Tarkastelen Raittiussanomien, Helsingin Sanomien ja Alkoholipolitiikka-aikakauskirjan kirjoittelua vuosina 1967 - 1968, jolloin uudet lait olivat eduskuntakäsittelyssä. Tutkimani kirjoitukset pyrkivät vaikuttamaan työn alla olleeseen lainsäädäntöön ja kansanedustajien mielipiteisiin lakiäänestyksen lähetessä. Helsingin Sanomat ja Alkoholipolitiikka osoittautuivat uusien lakien voimakkaiksi kannattajiksi ja läpiajajiksi, kun taas Raittiussanomien rooliksi jäi raittiusaatteen puolustaminen altavastaajan asemasta. Diskurssianalyysin välineillä pyrin löytämään keinoja kuinka omaa …

1960-lukualkoholilakialkoholipolitiikkaraittiusliikkeetolutargumentointiAlkoRaittiussanomatDiskurssianalyysiretoriikkaAlkoholipolitiikka (aikakauskirja)Helsingin Sanomat
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Rationalistinen muodonmuutos : Paula Pihkalan suunnittelemat asuinkerrostalot 1950- ja 1960-luvuilla Helsingissä ja Tammisaaressa

2001

1960-lukukaavoitusrakennusvalvontaPihkala Paularationalismirakennuslakikaupunkisuunnittelukerrostalot1950-lukuHaagarakennuselementitTammisaari
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Prospective, blinded comparison of cytology and DNA-image cytometry of brush biopsies for early detection of oral malignancy

2012

summary Objectives: Adjunctive techniques like DNA image cytometry (DNA-ICM) have been attributed to enhance the diagnostic performance of oral brush biopsies. The aim of the study was an evaluation of brush biopsies, analysed according to morphological criteria and by DNA-ICM vs. histological findings in a blinded prospective trial. Materials and methods: Eighty eight brush biopsies of 70 patients were sampled. Only clinical suspicious but not evident malignant oral lesions were included. Clinical diagnosis was leukoplakia (n = 36), lichen planus (n = 18), verruciform erythroplakia (n = 12), erythroleukoplakia (n = 9), erosion (n = 7) and induration (n = 6). Evaluation was conducted via hi…

AdultMaleCancer ResearchPathologymedicine.medical_specialtyCytodiagnosisMalignancySensitivity and SpecificityCytologyOral and maxillofacial pathologyImage Processing Computer-AssistedmedicineCarcinomaHumansSingle-Blind MethodProspective StudiesFalse Negative ReactionsMouth FloorEarly Detection of CancerAgedImage CytometryLeukoplakiaAged 80 and overErythroplakiaPloidiesbusiness.industryMouth MucosaHistologyDNA NeoplasmMiddle Agedmedicine.diseaseTongue Neoplasmsstomatognathic diseasesOncologyDysplasiaErythroplasiaCarcinoma Squamous CellFemaleMouth NeoplasmsLeukoplakia OralOral SurgerybusinessPrecancerous ConditionsCarcinoma in SituLichen Planus OralOral Oncology
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Stromal myofibroblasts in oral leukoplakia and oral squamous cell carcinoma

2011

Objectives: Oral leukoplakia (OL) is the main potentially malignant disorder and oral squamous cell carcinoma (OSCC) is the most common malignancy of the oral mucosa. Stromal myofibroblasts play an important role in tumor invasion and metastasis, due to its ability to modify the extracellular matrix. This study aimed to evaluate the presence of stromal myofibroblasts in OL and OSCC. Differences in the presence of myofibroblasts among OL with distinct grades of epithelial dysplasia as well as between histologically high- and low-invasive OSCC were also assessed. Study Design: A total of 30 OL and 41 OSCC from archival formalin-fixed, paraffin-embedded specimens were evaluated. 10 samples of …

AdultMaleEpithelial dysplasiaPathologymedicine.medical_specialtyStromal cellMetastasisCarcinomaMedicineHumansOral mucosaMyofibroblastsGeneral DentistryLeukoplakiaAgedMouth neoplasmAged 80 and overOral Medicine and Pathologybusiness.industryMiddle Agedmedicine.disease:CIENCIAS MÉDICAS [UNESCO]stomatognathic diseasesmedicine.anatomical_structureOtorhinolaryngologyUNESCO::CIENCIAS MÉDICASCarcinoma Squamous CellImmunohistochemistrySurgeryFemaleMouth NeoplasmsResearch-ArticleLeukoplakia Oralbusiness
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Detection of Epstein-Barr virus (EBV) DNA and antigens in oral mucosa of renal transplant patients without clinical evidence of oral hairy leukoplaki…

1998

The use of the polymerase chain reaction (PCR) to detect the presence of Epstein-Barr virus (EBV) DNA in oral mucosa in the absence of specific lesions gives rise to the problem of identifying the real viral replication sites. To verify whether the detection of EBV is due to salivary contamination or its true replicative capacity in oral mucosa, saliva samples and exfoliated cells from four different oral mucosa sites were taken from 40 renal transplant patients and 20 normal subjects for examination by PCR using two pairs of primers specific for the BamHI-L and BamHI-K genomic regions. EBV-specific sequences were detected in one or more of the oral mucosa samples from 29 transplant patient…

AdultMaleHairy leukoplakiaHerpesvirus 4 HumanCancer ResearchPathologymedicine.medical_specialtySalivaLeukoplakia HairyAdolescentCD4-CD8 RatioFluorescent Antibody TechniqueGenome ViralBiologyVirus Replicationmedicine.disease_causePolymerase Chain ReactionHerpesviridaePathology and Forensic Medicinelaw.inventionImmunocompromised HostlawmedicineHumansOral mucosaSalivaAntigens ViralIn Situ HybridizationPolymerase chain reactionOral hairy leukoplakiaMouth MucosaAntibodies MonoclonalHLA-DR AntigensSequence Analysis DNAMiddle Agedmedicine.diseaseKidney TransplantationEpstein–Barr virusImmunoglobulin ATransplantationBlotting Southernmedicine.anatomical_structureOtorhinolaryngologyImmunoglobulin GDNA ViralImmunologyPeriodonticsFemaleOral SurgeryJournal of Oral Pathology &amp; Medicine
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