Search results for "laser"
showing 10 items of 3161 documents
Inter-individual variability of protein patterns in saliva of healthy adults
2009
International audience; In order to document inter-individual variability in salivary protein patterns, unstimulated whole saliva was obtained from 12 subjects at 10 am and 3 pm of the same day. Saliva proteins were separated using two-dimensional gel electrophoresis, and semi-quantified using image analysis. One-way ANOVA was used to test the effects “time of sampling” and “subject”. Data were further explored by multivariate analyses (PCA, hierarchical clustering). Spots of interest were identified by mass spectrometry (MALDI-TOF MS/MS and nanoLC ESI-IT MS/MS). A dataset of 509 spots matched in all gels was obtained. There was no diurnal statistical effect on salivary patterns while inter…
Proteomics Differentiate Between Thyroid-Associated Orbitopathy and Dry Eye Syndrome.
2015
PURPOSE: In patients with thyroid-associated orbitopathy (TAO), the dry eye syndrome occurs frequently, and symptoms and signs of both disorders overlap making early and accurate differential diagnosis difficult. A differentiation via specific markers is warranted. METHODS: Tear fluid samples of 120 subjects with TAO, TAO + dry eye, dry eye, and controls were collected. The samples were measured using matrix-assisted laser desorption ionization mass spectrometry. The identified proteins were tested with antibody microarrays. RESULTS: Proteomics identified deregulated proteins in TAO and dry eye. Compared with dry eye, proline-rich protein 1 (PROL1, P = 0.002); uridine diphosphate (UDP)-gluc…
Proteomics of tear fluid in thyroid-associated orbitopathy.
2012
Proteomics and mass spectrometry are useful tools for peptide screening in body fluids. In thyroid-associated orbitopathy (TAO), evidence for lacrimal gland involvement with altered composition of tears has been reported. Our objective was to detect and evaluate potential changes in the proteomic patterns of tear fluid in TAO.Tear fluid was collected from 45 patients with TAO and 15 healthy controls. Tear proteins were analyzed using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, and peptides were identified using matrix-assisted laser desorption/ionization time-of-flight technology.Peptides with molecular weights 3808 Dalton (Da, p=0.004), 3734 Da (p=0.034),…
Influence of Refraction on Tonometric Readings After Photorefractive Keratectomy and Laser Assisted In Situ Keratomileusis
2000
PURPOSE To determine the decrease of Goldmann tonometry after photorefractive keratectomy (PRK) and laser assisted in situ keratomileusis (LASIK) according to refraction prior to surgery. METHODS Prospective simultaneous comparative case series of 53 myopic eyes (53 patients) that underwent PRK and 50 (50 patients) that underwent LASIK using the Summit Excimed SVS plus (Summit Technology, Inc., Walthan, MA, U.S.A.). PRK and LASIK were subdivided by refractive error into two subgroups of more than or less than -5 diopters (D). Central tonometric readings were taken prior to surgery and 12 months after surgery. RESULTS In LASIK and PRK subgroups of more than -5 D, 85.7% (24) and 69.6% (24), r…
Reproducibility of digital image analysis for measuring corneal haze after myopic photorefractive keratectomy.
1997
Purpose To evaluate the usefulness of digital image analysis for quantifying corneal haze by determining the reproducibility of its measurements at the corneal plane. Methods In a prospective study, 20 randomly selected eyes that had undergone myopic photorefractive keratectomy were photographed focusing the slit beam on their anterior corneal surface. Each photograph was examined using computer image analysis techniques that detect the edge of the reticular pattern of the image. Quantification of the difference between two areas, treated and adjacent untreated cornea, each containing 3,750 pixels with a resolution of 256 gray levels, was performed. Intra-analyzer variation was determined b…
Direct Objective Quantification of Corneal Haze after Excimer Laser Photorefractive Keratectomy for High Myopia
1996
Purpose: The purpose of the study is to measure regional distribution differences in corneal haze after excimer laser photorefractive keratectomy for high myopia. Methods: The authors developed computerized gradient edge detectors with which were analyzed digitized anterior slit-lamp photographs of 40 eyes, an average of 21.0 plus or minus 14.5 weeks after photorefractive keratectomy for high myopia (−6 to −22 diopters). A treated area and an adjacent untreated area on the anterior corneal surface, each containing six regions, were quantified, and the difference was correlated with various parameters. Results: Mean differences between scarred and clear areas for haze grade 0.5, 1.0, 2.0, 3.…
Mesopic contrast sensitivity function after excimer laser photorefractive keratectomy.
2002
ABSTRACT PURPOSE: To evaluate contrast sensitivity under mesopic conditions in patients who had undergone uncomplicated excimer laser photorefractive keratectomy (PRK) for myopia. METHODS: Monocular contrast sensitivity function was measured with the Stereo Optical ?A.C.T. chart in 26 patients who had received PRK using the Nidek EC-5000 excimer laser system. Mean preoperative refractive error was -6.23 ? 1.69 D (range, -4.00 to -8.25 D); postoperatively, mean refractive error was -0.36 ? 0.58 D (range, -0.75 to +0.50 D). Contrast sensitivity function was measured 6 months after surgery using four different chart luminances: 85, 5.0, 2.5, and 0.1 cd/m2, the first being a photopic level and …
Mesopic Contrast Sensitivity Function After Laser in situ Keratomileusis
2003
ABSTRACT PURPOSE: To evaluate contrast sensitivity under mesopic conditions in patients who have undergone uncomplicated bilateral laser in situ keratomileusis (LASIK) for myopia. METHODS: Best-corrected monocular contrast sensitivity was measured with the Stereo Optical F.A.C.T. chart at 1.5, 3, 6, 12, and 18 c/deg in 20 patients (20 left eyes) before and after bilateral LASIK (with the Nidek EC-5000 excimer laser and the Automated Corneal Shaper). Mean preoperative refractive error was -6.40 ± 1.28 D (range -4.00 to -8.00 D) and postoperative mean refractive error was -0.41 ± 0.45 D (range -0.75 to +0.50 D). Contrast sensitivity function was measured before and 6 months after surgery usin…
Excimer laser photorefractive keratectomy for high myopia
1995
Abstract One hundred and thirty-three eyes of 103 patients had photorefractive keratectomy with a slit scan mode excimer laser for myopia ranging from −6.00 to −22.00 diopters (D). The epithelium was removed with 20% ethanol, and the ablation was done with a tapered profile surrounding the optical zone. Patients were divided into two groups based on preoperative myopia: Group A, −6.00 D to −12.00 D (88 eyes); Group B, −12.50 D to −22.00 D (45 eyes). In Group A, mean preoperative refraction was −9.59 ± 1.79 D. Mean postoperative refraction was −0.29 ± 1.47 D at one month, −0.85 ± 1.68 D at three months, −1.17 ± 2.04 D at six months, and −0.56 ± 0.74 D at one year. Anterior stromal haze was g…
Characterization of the effect of intracorneal ring segment in corneal ectasia after laser refractive surgery
2018
Purpose: To evaluate the visual, refractive, topographic, pachymetric, and biomechanical outcomes after intracorneal ring segment implantation in corneas with post-LASIK ectasia. Methods: Retrospective longitudinal study enrolling 26 eyes of 22 patients with post-LASIK ectasia and undergoing intracorneal ring segment implantation (KeraRing®, Mediphacos) using a 60-kHz femtosecond laser (IntraLase®, IntraLase Corp.) for corneal tunnelization. Visual, refractive, anterior, and posterior corneal topographic (Pentacam HR, Oculus), pachymetric, and corneal biomechanical changes (Ocular response Analyzer, Reichert) were evaluated during a 12-month follow-up. Vector analysis of astigmatic changes …