Search results for "layer"
showing 10 items of 2667 documents
Combining a Two-Source Patch Model with Satellite Data to Monitor Daily Evapotranspiration at a Regional Scale
2008
In this work, we present a micro-meteorological approach for estimating surface energy fluxes that can be operationally used together with satellite images to monitor surface energy fluxes at a regional scale. In particular we will focus on the retrieval of daily evapotranspiration. The feasibility of the model is explored at a local scale using data collected over a maize crop in Beltsville, Maryland, USA, and a boreal forest in Sodankyla, Finland. Comparison of the results with ground measurements shows errors between plusmn15 and plusmn50 W m-2 for the retrieval of net radiation, soil heat flux, and sensible and latent heat fluxes in both sites. A methodology to apply the model to Landsa…
Genes and derived amino acid sequences of S-layer proteins from mesophilic, thermophilic, and extremely thermophilic methanococci
2002
Cells of methanococci are covered by a single layer of protein subunits (S-layer) in hexagonal arrangement, which are directly exposed to the environment and which cannot be stabilized by cellular components. We have isolated S-layer proteins from cells of Methanococcus vannielii ( T(opt.)=37 degrees C), Methanococcus thermolithotrophicus ( T(opt.)=65 degrees C), and Methanococcus jannaschii ( T(opt.)=85 degrees C). The primary structure of the S-layer proteins was determined by sequencing the corresponding genes. According to the predicted amino acid sequence, the molecular masses of the S-layer proteins of the different methanococci are in a small range between 59,064 and 60,547 Da. Compa…
Surface, mechanical and barrier properties of bio-based composite films based on chitosan and whey protein
2014
Mono-component and composite bilayer and blend films composed of chitosan and whey protein were made. Colour, microstructure, water contact angles, swelling, water vapour sorption, barrier properties (oxygen, water vapour), water vapour diffusion coefficients and mechanical properties were determined. The influence of water vapour on barrier properties was studied in relation to the surface and structural properties. Mono-component and bilayer films were transparent with a homogeneous surface. Contrarily, blend films were translucent. Bilayer films had significantly lower water vapour permeability in comparison to mono-component and blend films. In all bilayer films, the air side (chitosan)…
MEMS analogous micro-patterning of thermotropic nematic liquid crystalline elastomer films using a fluorinated photoresist and a hard mask process
2017
In this work, we present a method to pattern liquid crystal elastomers (LCEs) in the micrometer range without using any mechanical processing steps to prepare micron sized LCE actuators compatible with microelectromechanical system (MEMS) technology. Multi-layer spin-coating processes are developed to synthesise and structure 300–3500 nm thick LCE films. A water soluble sacrificial layer, a photoalignment layer and a LCE formulation, which is polymerised and crosslinked in its liquid crystal phase, are spin-coated successively onto a substrate. A fluorinated photoresist layer is used to structure LCE films with thicknesses up to 700 nm in a photolithographic and etching process. For thicker…
Preparation of Ta-O-Based Tunnel Junctions to Obtain Artificial Synapses Based on Memristive Switching
2014
Magnetron sputtering and optical lithography are standard techniques to prepare magnetic tunnel junctions with lateral dimensions in the micrometer range. Here we present the materials and techniques to deposit the layer stacks, define the structures, and etch the devices. In the end, we obtain tunnel junction devices exhibiting memristive switching for potential use as artificial synapses.
Domain Structures in Langmuir-Blodgett Films Investigated by Atomic Force Microscopy
1993
Investigations of phase-separated Langmuir-Blodgett films by atomic force microscopy reveal that on a scale of 30 to 200 micrometers, these images resemble those observed by fluorescence microscopy. Fine structures (less than 1 micrometer) within the stearic acid domains were observed, which cannot be seen by conventional optical microscopic techniques. By applying the force modulation technique, it was found that the elastic properties of the domains in the liquid condensed phase and grains observed within the liquid expanded phase were comparable. Small soft residues in the domains could also be detected. The influence of trace amounts of a fluorescence dye on the micromorphology of monol…
Liquid crystal lens array for 3D microscopy and endoscope application
2016
In this paper, we demonstrate two liquid crystal (LC) lens array devices for 3D microscope and 3D endoscope applications respectively. Compared with the previous 3D biomedical system, the proposed LC lens arrays are not only switchable between 2D and 3D modes, but also are able to adjust focus in both modes. The multi-function liquid crystal lens (MFLC-lens) array with dual layer electrode has diameter 1.42 mm, which is much smaller than the conventional 3D endoscope with double fixed lenses. The hexagonal liquid crystal micro-lens array (HLC-MLA) instead of fixed micro-lens array in 3D light field microscope can extend the effective depth of field from 60 um to 780 um. To achieve the LC le…
Dynamic behaviors of fractal-like domains in monolayers
1996
In this paper we report our recent investigations on the morphological evolution of fractal-like domains of the liquid-condensed (LC) phase in lipid monolayers. It is demonstrated that the dimension of the LC domains increases upon continuous illumination of microscope light. The experimental data indicate that the increasing rate of fractal dimension of the LC domains depends on the concentration of fluorescence probes. By analyzing the domain growth process we find that the self-similarity of the pattern disappears gradually during its growth. The possible mechanism behind the observed phenomena is discussed.
Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores
2006
Proteins of the B-cell lymphoma protein 2 (Bcl2) family are key regulators of the apoptotic cascade, controlling the release of apoptotic factors from the mitochondrial intermembrane space. A helical hairpin found in the core of water-soluble folds of these proteins has been reported to be the pore- forming domain. Here we show that peptides including any of the two a-helix fragments of the hairpin of Bcl2 associated protein X (Bax) can independently induce release of large labelled dextrans from synthetic lipid vesicles. The permeability promoted by these peptides is influenced by intrinsic monolayer curvature and accompanied by fast transbilayer redis- tribution of lipids, supporting a to…
Selective Gas and Vapor Sorption and Magnetic Sensing by an Isoreticular Mixed-Metal–Organic Framework
2012
A novel isoreticular oxamato-based manganese(II)-copper(II) open metal-organic framework H(2)O@iso1 featuring a pillared square/octagonal layer structure with alternating open and closed octagonal pores has been rationally prepared. The open-framework topology is responsible for a large selectivity in the separation of small gas (CO(2) over CH(4)) and vapor molecules (CH(3)OH over CH(3)CN and CH(3)CH(2)OH). H(2)O@iso1 displays a long-range three-dimensional ferromagnetic ordering with a drastic variation of the critical temperature as a function of the guest molecule [T(C)2.0 K (CO(2)@iso1 and CH(4)@iso1) and T(C) = 6.5 (CH(3)OH@iso1) and 21.0 K (H(2)O@iso1)].