Search results for "link"
showing 10 items of 1973 documents
Monitoring of transglutaminase crosslinking reaction by 1H NMR spectroscopy on model substrates
2015
International audience; A new method based on 1H NMR spectroscopy was developed for monitoring transglutaminase crosslinking reaction with model molecules (CBZ-Gln-Gly and N-α-acetyl-lysine). The transglutaminase reaction led to the appearance of new resonances on NMR spectrum as well as significant decrease in others. The new observed resonances, originated from newly formed ɛ-(γ-glutamyl)lysine isopeptide bonds, evidence the enzymatic reaction and allow to quantify the ɛ-(γ-glutamyl)lysine fragment. Moreover, the decrease in resonance intensity, originated from lysine, permit to determine the crosslinking degree. These results obtained by 1H NMR spectroscopy can be used as an alternative …
Fish introductions and light modulate food web fluxes in tropical streams: a whole-ecosystem experimental approach.
2016
Decades of ecological study have demonstrated the importance of top-down and bottom-up controls on food webs, yet few studies within this context have quantified the magnitude of energy and material fluxes at the whole-ecosystem scale. We examined top-down and bottom-up effects on food web fluxes using a field experiment that manipulated the presence of a consumer, the Trinidadian guppy Poecilia reticulata, and the production of basal resources by thinning the riparian forest canopy to increase incident light. To gauge the effects of these reach-scale manipulations on food web fluxes, we used a nitrogen (15 N) stable isotope tracer to compare basal resource treatments (thinned canopy vs. co…
Multiple‐batch spawning as a bet‐hedging strategy in highly stochastic environments: An exploratory analysis of Atlantic cod
2021
Stochastic environments shape life‐history traits and can promote selection for risk‐spreading strategies, such as bet‐hedging. Although the strategy has often been hypothesised to exist for various species, empirical tests providing firm evidence have been rare, mainly due to the challenge in tracking fitness across generations. Here, we take a ‘proof of principle’ approach to explore whether the reproductive strategy of multiple‐batch spawning constitutes a bet‐hedging. We used Atlantic cod (Gadus morhua) as the study species and parameterised an eco‐evolutionary model, using empirical data on size‐related reproductive and survival traits. To evaluate the fitness benefits of multiple‐batc…
Wild
2021
Graphical abstract
Nucleotide Variability at the Acetyl Coenzyme A Carboxylase Gene and the Signature of Herbicide Selection in the Grass Weed Alopecurus myosuroides (H…
2004
Acetyl coenzyme A carboxylase (ACCase) is the target of highly effective herbicides. We investigated the nucleotide variability of the ACCase gene in a sample of 18 black-grass (Alopecurus myosuroides [Huds.]) populations to search for the signature of herbicide selection. Sequencing 3,396 bp encompassing ACCase herbicide-binding domain in 86 individuals revealed 92 polymorphisms, which formed 72 haplotypes. The ratio of nonsynonymous versus synonymous substitutions was very low, in agreement with ACCase being a vital metabolic enzyme. Within black grass, most nonsynonymous substitutions were related to resistance to ACCase-inhibiting herbicides. Differentiation between populations was stro…
Grazing decreases N partitioning among coexisting plant species
2017
Herbivores play a key role in shaping ecosystem structure and functions by influencing plant and microbial community composition and nutrient cycling. This study investigated the long‐term effects of herbivores on plant resource acquisition. We explored differences in the natural δ15N signatures in plant, microbial and soil N pools, and examined mycorrhizal colonization in two tundra sites that have been either lightly or heavily grazed by reindeer for more than 50 years. The study examined changes in nutrient acquisition in five common tundra plants with contrasting traits and mycorrhiza status; the mycorrhizal dwarf shrubs, Betula nana, Vaccinium myrtillus and Empetrum hermaphroditum; a m…
New polymorphic microsatellite loci in the house sparrow, Passer domesticus.
2009
3 pages; International audience; We developed 13 new polymorphic microsatellite loci in the house sparrow (Passer domesticus), which exhibited from 2 to 15 alleles. Observed and expected heterozygosities ranged from 0.17 to 0.77 and from 0.35 to 0.85, respectively. We detected no linkage disequilibrium between loci. Allele frequencies supported Hardy–Weinberg equilibrium for 8 loci out of 13 after Bonferroni correction. Combined with loci previously isolated in the house sparrow, these new microsatellite markers provide valuable tools to study population genetics of this species.
Heterozygosity-fitness correlations in adult and juvenile Zenaida Dove, Zenaida aurita.
2013
10 pages; International audience; Understanding how fitness is related to genetic variation is of crucial importance in both evolutionary ecology and conservation biology. We report a study of heterozygosity-fitness correlations in a wild, noninbred population of Zenaida Doves, Zenaida aurita, based on a sample comprising 489 individuals (382 adults and 107 juveniles) typed at 13 microsatellite loci, resulting in a data set comprising 5793 genotypes. In both adults and juveniles, and irrespective of sex, no evidence was found for an effect of either multilocus or single-locus heterozygosity on traits potentially related to fitness such as foraging tactic, competitive ability, and fluctuatin…
The effect of fish life-history structures on the topologies of aquatic food webs
2021
Biological organisms can vastly change their ecological functionality due to changes in body size and diet across their life. Consequently, it has been increasingly recognized that to attain sufficient biological realism, food webs may need to include life-history structures. The objective of the work is to study theoretically whether and how the inclusion of life-history structures affects the food web topology. Topological research was done by applying network theory metrics for three different food web types with two different sizes that were generated by using the niche-model. The dynamical modeling was performed by using an allometric trophic network modeling approach. The different ty…
Species interactions, environmental gradients and body size shape population niche width
2021
1. Competition for shared resources is commonly assumed to restrict population-level niche width of coexisting species. However, the identity and abundance of coexisting species, the prevailing environmental conditions, and the individual body size may shape the effects of interspecific interactions on species’ niche width. 2. Here we study the effects of interspecific and intraspecific interactions, lake area and altitude, and fish body size on the trophic niche width and resource use of a generalist predator, the littoral-dwelling large, sparsely rakered morph of European whitefish (Coregonus lavaretus; hereafter LSR whitefish). We use stable isotope, diet and survey fishing data from 14 …