Search results for "liquid"

An Optimized and Rapid DNA Extraction Method From Leaves of Grapevine Suitable for PCR-DGGE Based Analysis

2010

Molecular techniques and genetic studies require a fair amount of DNA of high quality in order to produce reliable and clear results (Li et al., 2007). In grapevine, the yield and quality of DNA can be significantly affected by secondary metabolites such as polyphenols, polysaccharides and tannins, that may be consistently abundant during the different stages of leaf development (Iandolino et al., 2004). The development of a simple, rapid and reliable method for the extraction of genomic DNA from grape leaves collected at several stages during development, as well as from healthy grape leaves and leaves infected by pathogenic microorganisms, was the main objective of this study. The protoco…

Gasification of pelletized renewable fuel for clean energy production

2011

Abstract The main aim of the study was to develop and investigate a small-scale experimental gasification technique for the effective thermal decomposition of pelletized renewable fuels (wood sawdust, wheat straw). The technical solution of the biomass gasifier for gasification of renewable fuels presents a downdraft gasifier with controllable additional heat energy supply to the biomass using the radial propane flame injection into the bottom part of the biomass layer. From the kinetic study of the mass conversion rate of pelletized biomass and variations of the composition of produced gas it is concluded that the process of biomass gasification is strongly influenced by the amount of addi…

Microfluidic Synthesis of Liquid Crystalline Elastomer Particle Transport Systems which Can Be Remote‐Controlled Magnetically

2019

Cover Picture: Nanostructuring with a Crosslinkable Discotic Material (Small 8/2007)

2007

How Ionic Liquid Gels Work on the Removal of Bisphenol A from Wastewater

2022

The occurrence of emerging pollutants in water bodies is a pressing issue of modern society and identifying materials to remove them is the main target of current research. In this work, we prepared and characterized supramolecular gels of 1,3:2,4-dibenzylidene-d-sorbitol (DBS) in ionic liquids differing for the anion and the aliphatic or aromatic nature of the cation. We characterized our gels for their thermal stability and mechanical properties. We also found that all gels self-heal in 24 h after being cut by a razor blade. We then used our gels as sorbents to remove bisphenol A, an endocrine disruptor compound, from aqueous solutions. All gels adsorb BPA with high removal efficiencies, …

The shell matrix of the european thorny oyster, Spondylus gaederopus: microstructural and molecular characterization.

2020

17 pages; International audience; Molluscs, the largest marine phylum, display extraordinary shell diversity and sophisticated biomineral architectures. However, mineral-associated biomolecules involved in biomineralization are still poorly characterised.We report the first comprehensive structural and biomolecular study of Spondylus gaederopus, a pectinoid bivalve with a peculiar shell texture. Used since prehistoric times, this is the best-known shell of Europe’s cultural heritage. We find that Spondylus microstructure is very poor in mineral-bound organics, which are mostly intercrystalline and concentrated at the interface between structural layers.Using high-resolution liquid chromatog…

Pulsed electric fields (PEF), pressurized liquid extraction (PLE) and combined PEF + PLE process evaluation: Effects on Spirulina microstructure, bio…

2022

This study aims at evaluating the impact of different processes-pulsed electric fields (PEF), pressurized liquid extraction (PLE) and a multistep process combining PEF + PLE on the yield of antioxidant compounds (protein, polyphenols, chlorophyll a, chlorophyll b, and carotenoids) from Spirulina. Firstly, the effects of PEF or PLE treatment on the extraction yield of Spirulina biomolecules were evaluated. To further increase the extraction yield, PEF + PLE was used, as an innovative extraction approach. The results showed that PEF + PLE greatly improved the extraction yield compared with the PEF or PLE treatments alone. Compared with Folch extraction (conventional control technique), PEF + …

UDP-glucosyltransferase activity toward exogenous substrates in Drosophila melanogaster.

1991

To investigate the capacity of Drosophila extracts to glucosylate exogenous substrates we have developed a fast and sensitive method for the detection of UDP-glucosyltransferase activity using 4-nitrophenol, 1-naphthol, or 2-naphthol as substrates. High-performance liquid chromatography was used to separate and quantitate the reaction products, allowing detection of activities that produced as little as 1 pmol of 2-naphthol glucoside (fluorescence detection) or 16 pmol of 4-nitrophenol glucoside (absorbance detection). Optimal activity was found at 43 degrees C and alkaline pH. The affinity of the Drosophila enzyme was 250-fold higher for 1-naphthol or 2-naphthol (Km approximately 4 microM)…

Bio-polymeric Ionic Liquid Gels for the Desulfurization of Fuel

Metabolomic Diversity of Human Milk Cells over the Course of Lactation—A Preliminary Study

2023

Human milk (HM) is a complex biofluid containing a wide cell variety including epithelial cells and leukocytes. However, the cellular compositions and their phenotypic properties over the course of lactation are poorly understood. The aim of this preliminary study was to characterize the cellular metabolome of HM over the course of lactation. Cells were isolated via centrifugation and the cellular fraction was characterized via cytomorphology and immunocytochemical staining. Cell metabolites were extracted and analyzed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC–QqTOF-MS) in the positive and negative electrospray ionization mode…