Search results for "localization"
showing 10 items of 443 documents
Nanoscale distribution of TLR4 on primary human macrophages stimulated with LPS and ATI
2019
Toll-like receptor 4 (TLR4) plays a crucial role in the recognition of invading pathogens. Upon activation by lipopolysaccharides (LPS), TLR4 is recruited into specific membrane domains and dimerizes. In addition to LPS, TLR4 can be stimulated by wheat amylase-trypsin inhibitors (ATI). ATI are proteins associated with gluten containing grains, whose ingestion promotes intestinal and extraintestinal inflammation. However, the effect of ATI vs. LPS on the membrane distribution of TLR4 at the nanoscale has not been analyzed. In this study, we investigated the effect of LPS and ATI stimulation on the membrane distribution of TLR4 in primary human macrophages using single molecule localization m…
An international genome-wide meta-analysis of primary biliary cholangitis: Novel risk loci and candidate drugs.
2021
[BACKGROUND & AIMS] Primary biliary cholangitis (PBC) is a chronic liver disease in which autoimmune destruction of the small intra-hepatic bile ducts eventually leads to cirrhosis. Many patients have inadequate response to licensed medications, motivating the search for novel therapies. Previous genome-wide association studies (GWAS) and meta-analyses (GWMA) of PBC have identified numerous risk loci for this condition, providing insight into its aetiology. We undertook the largest GWMA of PBC to date, aiming to identify additional risk loci and prioritise candidate genes for in silico drug efficacy screening. [METHODS] We combined new and existing genotype data for 10, 516 cases and 20, 77…
Apprentissage de modalités auxiliaires pour la localisation basée vision
2018
In this paper we present a new training with side modality framework to enhance image-based localization. In order to learn side modality information, we train a fully convo-lutional decoder network that transfers meaningful information from one modality to another. We validate our approach on a challenging urban dataset. Experiments show that our system is able to enhance a purely image-based system by properly learning appearance of a side modality. Compared to state-of-the-art methods, the proposed network is lighter and faster to train, while producing comparable results.
Polyphased mesozoic rifting from the Atlas to the north-west Africa paleomargin
2021
24 pages; International audience; Based on the interpretation of geological maps, seismic reflection and well data complemented with a bibliographic compilation and field work in the Rif, we investigate the factors that control the rift initiation, its development and the formation of oceanic crust in NW Africa. From SE to NW, we examine the Western Sahara Atlas, the Tendrara, the Guercif, and the Rif basins, to establish their geodynamic evolution in relation with the Mesozoic formation of the Central Atlantic and Maghrebian Tethys oceans, respectively. The Triassic extension was diffuse and developed over Lower Carboniferous horst-and-graben structures formed in the NW passive margin of G…
THE COST AS THE MAIN DETERMINANT OF THE LOCATION OF LOGSTICS CENTERS
2018
Apropriate location of the logistics center allows entrepreneurs to significantly reduce the costs of transport. On the other hand, it has a positive impact on the development of the transport infrastructure of the region.This paper presents the practical aspects of the localization problem of logistics center with the use of a geographic information system. It shows differences between theoretical models, the needs of decision-makers, and the data available to make the necessary calculations. The results of computational experiments were introduced to illustrate the effects of this approach.
Synthesis and In Vitro Evaluation of Defined HPMA Folate Conjugates: Influence of Aggregation on Folate Receptor (FR) Mediated Cellular Uptake
2010
In this article we report the synthesis and in vitro evaluation of well-defined, folate functionalized and fluorescently labeled polymers based on the clinically approved N-(2-hydroxypropyl)-methacrylamide (HPMA). The polymers were prepared applying the RAFT polymerization method as well as the reactive ester approach. The molecular weights of the polymers synthesized were around 15 and 30 kDa. The total content of conjugated folate varied from 0, 5, and 10 mol %. The cellular uptake of these polymers was investigated in the folate receptor (FR)-positive human nasopharyngeal epidermal carcinoma (KB-3-1) and FR-negative human lung epithelial carcinoma (A549) cancer cell lines. In FR-positive…
S-nitrosylation of the death receptor fas promotes fas ligand-mediated apoptosis in cancer cells.
2011
International audience; BACKGROUND & AIMS: Fas belongs to the family of tumor necrosis factor receptors which induce apoptosis. Many cancer cells express Fas but do not undergo Fas-mediated apoptosis. Nitric oxide reverses this resistance by increasing levels of Fas at the plasma membrane. We studied the mechanisms by which NO affects Fas function. METHODS: Colon and mammary cancer cell lines were incubated with the NO donor glyceryl trinitrate or lipid A; S-nitrosylation of Fas was monitored using the biotin switch assay. Fas constructs that contained mutations at cysteine residues that prevent S-nitrosylation were used to investigate the involvement of S-nitrosylation in Fas-mediated cell…
Technics Amélioration for geo-localization in wsn via smart computing and real time application
2022
New technologies exploiting digital information acquisition by radio frequency techniques are now commonly used in various practical fields. They are most often used to measure a variety of physical variables such as temperature, humidity, speed, etc. and are gathered under the name of Wireless Sensor Networks “WSN”. For this variant of applications, the accurate location of connected sensor nodes remains an important issue for researchers and industrial applications. Indeed, existing localization algorithms can be classified into two categories known as « range-based » and « range-free ». Range-based localization systems are characterized by major drawbacks. The first one is the cost of th…
Aminopropyltransferases involved in polyamine biosynthesis localize preferentially in the nucleus of plant cells
2012
Plant aminopropyltransferases consist of a group of enzymes that transfer aminopropyl groups derived from decarboxylated S-adenosyl-methionine (dcAdoMet or dcSAM) to propylamine acceptors to produce polyamines, ubiquitous metabolites with positive charge at physiological pH. Spermidine synthase (SPDS) uses putrescine as amino acceptor to form spermidine, whereas spermine synthase (SPMS) and thermospermine synthase (TSPMS) use spermidine as acceptor to synthesize the isomers spermine and thermospermine respectively. In previous work it was shown that both SPDS1 and SPDS2 can physically interact with SPMS although no data concerning the subcellular localization was reported. Here we study the…
Serine/threonine-kinase 33 (Stk33) – Component of the neuroendocrine network?
2016
The present study was conducted to investigate the expression of serine/threonine-kinase 33 (Stk33) in neuronal structures of the central nervous system in rat and hamster as well as the presence of the protein in the brain of higher mammals, using a polyclonal antibody on cryosections of fixed brains. We found a distinct immunostaining pattern that included intense fluorescence of the ependymal lining of cerebral ventricles, and of hypothalamic tanycytes and their processes. We further observed intense staining of magnocellular neurons in the hypothalamic paraventricular, supraoptic and accessory neurosecretory nuclei, in particular the circular nuclei, and less intense stained neurons in …