Search results for "lääkeresistenssi"

Adapting a Phage to Combat Phage Resistance

2020

Phage therapy is becoming a widely recognized alternative for fighting pathogenic bacteria due to increasing antibiotic resistance problems. However, one of the common concerns related to the use of phages is the evolution of bacterial resistance against the phages, putatively disabling the treatment. Experimental adaptation of the phage (phage training) to infect a resistant host has been used to combat this problem. Yet, there is very little information on the trade-offs of phage infectivity and host range. Here we co-cultured a myophage FCV-1 with its host, the fish pathogen Flavobacterium columnare, in lake water and monitored the interaction for a one-month period. Phage resistance was…

Small things matter : of phages and antibiotic resistance conferring plasmids

2016

Viruses and plasmids are small units of genetic material dependent on cells either transiently or continuously. Intriguingly, stories of these small entities intertwine in antibiotic resistance crisis. Horizontal gene transfer enables bacteria to respond rapidly to chances in their environment. Anthropogenic consumption of antibiotics induces the travel of resistance encoding genes mainly as passengers of conjugative plasmids. In this thesis, I demonstrate that clinically important resistance plasmids could evolutionarily rescue susceptible bacteria under lethal antibiotic concentrations. If mobile resistance genes are available in surrounding community, administration of high doses of anti…

Counteracting the horizontal spread of bacterial antibiotic resistance with conjugative plasmid-dependent bacteriophages

2016

Expert Opinion on Three Phage Therapy Related Topics: Bacterial Phage Resistance, Phage Training and Prophages in Bacterial Production Strain

2018

Expert Opinion on Three Phage Therapy Related Topics: Bacterial Phage Resistance, Phage Training and Prophages in Bacterial Production Strain

2018

Phage therapy is increasingly put forward as a “new” potential tool in the fight against antibiotic resistant infections. During the “Centennial Celebration of Bacteriophage Research” conference in Tbilisi, Georgia on 26–29 June 2017, an international group of phage researchers committed to elaborate an expert opinion on three contentious phage therapy related issues that are hampering clinical progress in the field of phage therapy. This paper explores and discusses bacterial phage resistance, phage training and the presence of prophages in bacterial production strains while reviewing relevant research findings and experiences. Our purpose is to inform phage therapy stakeholders such as po…