Search results for "methods"

showing 10 items of 4526 documents

A co-registration investigation of inter-word spacing and parafoveal preview: Eye movements and fixation-related potentials

2019

Participants’ eye movements (EMs) and EEG signal were simultaneously recorded to examine foveal and parafoveal processing during sentence reading. All the words in the sentence were manipulated for inter-word spacing (intact spaces vs. spaces replaced by a random letter) and parafoveal preview (identical preview vs. random letter string preview). We observed disruption for unspaced text and invalid preview conditions in both EMs and fixation-related potentials (FRPs). Unspaced and invalid preview conditions received longer reading times than spaced and valid preview conditions. In addition, the FRP data showed that unspaced previews disrupted reading in earlier time windows of analysis, com…

MaleTime FactorsEye MovementsPhysiologyVisual SystemVisionComputer scienceSpeech recognitionSensory PhysiologyVisual PhysiologySocial ScienceslukeminensilmänliikkeetOcular physiology0302 clinical medicineFovealMedicine and Health SciencesPsychologyAttentionMacula LuteaEEGNeurolinguisticsClinical NeurophysiologyBrain MappingMultidisciplinaryQ05 social sciencesRElectroencephalographyHealthy VolunteersSensory SystemsSemanticsElectrophysiologyBioassays and Physiological AnalysisPattern Recognition VisualBrain ElectrophysiologyPhysical SciencestekstinymmärtäminenMedicineFemaleSensory PerceptionAnatomyResearch ArticleAdultAdolescentImaging TechniquesPermutationScienceNeurophysiologyCo registrationNeuroimagingFixation OcularResearch and Analysis Methods050105 experimental psychologyYoung Adult03 medical and health sciencesHumans0501 psychology and cognitive sciencesScalpDiscrete MathematicsElectrophysiological TechniquesCognitive PsychologyBiology and Life SciencesEye movementLinguisticsReadingSentence ProcessingCombinatoricsFixation (visual)katseenseurantaCognitive ScienceClinical MedicineHeadMathematics030217 neurology & neurosurgeryNeurosciencePLOS ONE
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Effects of work-interval duration and sport specificity on blood lactate concentration, heart rate and perceptual responses during high intensity int…

2018

The aim of this study was to examine the impacts on blood lactate concentration, measured heart rate and assessment of perceived exertion during split sessions of equal relative load, as also their relationship to the specific sport practised. Nineteen regional-level athletes (nine middle and long-distance runners (cyclic group) and ten field-sport team players (acyclic group)) performed four high-intensity interval training (HIIT) sessions with work-interval durations of 10 s, 50 s, 90 s and 130 s. The sessions were carried out at their usual training sites with a separation of at least 48 hours. Blood lactate concentration was measured at rest and 3 min after the completion of each protoc…

MaleTime FactorsPhysiologySocial Scienceslcsh:MedicineInterval trainingRunningMathematical and Statistical Techniques0302 clinical medicineHeart RateMedicine and Health SciencesBlood lactatePsychologyPublic and Occupational Health030212 general & internal medicinelcsh:ScienceRating of perceived exertionMultidisciplinarySports ScienceBody FluidsBloodDuration (music)Physical SciencesCardiologySensory PerceptionFemaleAnalysis of varianceAnatomyHigh-intensity interval trainingStatistics (Mathematics)Research ArticleSportsAdultmedicine.medical_specialtyCardiologyResearch and Analysis Methods03 medical and health sciencesInternal medicineHeart ratemedicineHumansLactic AcidSports and Exercise MedicineStatistical MethodsExerciseBehaviorAnalysis of VarianceBiological Locomotionbusiness.industryWork (physics)lcsh:RBiology and Life SciencesPhysical Activity030229 sport sciencesPhysical FitnessAthletesRecreationlcsh:QbusinessMathematicsNeurosciencePLoS ONE
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Supplementing sleep actigraphy with button pressing while awake

2020

Objective/backgroundWrist-worn sleep actigraphs are limited for evaluating sleep, especially in sleepers who lie awake in bed without moving for extended periods. Sleep logs depend on the accuracy of perceiving and remembering times of being awake. Here we evaluated pressing an event-marker button while lying awake under two conditions: self-initiated pressing every 5 to 10 minutes or pressing when signaled every 5 minutes by a vibration pulse from a wristband. We evaluated the two conditions for acceptability and their concordance with actigraphically scored sleep.Participants and methodsTwenty-nine adults wore actigraphs on six nights. On nights 1 and 4, they pressed the marker to a vibra…

MaleTime FactorsPhysiologyTest StatisticsWalkingAudiologyMathematical and Statistical Techniques0302 clinical medicineMedicine and Health SciencesMusculoskeletal SystemClinical NeurophysiologyCognitive ImpairmentBrain MappingMultidisciplinaryCognitive NeurologyPhysicsQStatisticsRClassical MechanicsElectroencephalographyMiddle AgedWristElectrophysiologyArmsBioassays and Physiological AnalysisBrain ElectrophysiologyNeurologyPhysical SciencesMedicineFemaleSleep (system call)AnatomyResearch ArticleAdultmedicine.medical_specialtyImaging TechniquesScienceCognitive NeuroscienceNeurophysiologyNeuroimagingResearch and Analysis MethodsVibrationButton pressingEvery 5 minutesYoung Adult03 medical and health sciencesmedicineHumansWakefulnessStatistical MethodsAgedBiological Locomotionbusiness.industryElectrophysiological TechniquesBiology and Life SciencesActigraphyActigraphySleep scoringEvery Morning030228 respiratory systemBody LimbsCognitive ScienceSleep onset latencyClinical MedicineSleepPhysiological ProcessesbusinessMathematics030217 neurology & neurosurgeryNeurosciencePLOS ONE
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Testing physiologic monitor alarm customization software to reduce alarm rates and improve nurses’ experience of alarms in a medical intensive care u…

2018

BACKGROUND:Clinicians in intensive care units experience alarm fatigue related to frequent false and non-actionable alarms produced by physiologic monitors. To reduce non-actionable alarms, alarm settings may need to be customized for individual patients; however, nurses may not customize alarms because of competing demands and alarm fatigue. OBJECTIVE:To examine the effectiveness and acceptance of physiologic monitor software to support customization of alarms. METHODS:This pre/post intervention study was conducted in a 56-bed medical intensive care unit. IntelliVue® Alarm Advisor customization support software for alarm limit violations was installed on all monitors and education on its u…

MaleTime FactorsPolymersHealth Care ProvidersRespiratory Tract Diseaseslcsh:MedicineNursesBlood PressureSurveysPathology and Laboratory Medicine01 natural sciencesVascular MedicinePersonalization0302 clinical medicineSoftwareHeart RateSoftware DesignMedicine and Health SciencesMedicine030212 general & internal medicineMedical Personnellcsh:SciencePolyvinyl ChlorideMaterialsFatigueMultidisciplinarySoftware EngineeringMiddle AgedHospitalsProfessionsIntensive Care UnitsChemistryMacromoleculesResearch DesignClinical AlarmsPhysical SciencesEngineering and TechnologyEquipment FailureFemaleMedical emergencyResearch ArticleComputer and Information SciencesMaterials ScienceCardiologyResearch and Analysis MethodsCommunicable DiseasesSoftware implementation03 medical and health sciencesALARMSigns and SymptomsRespiratory RateDiagnostic MedicineIntensive careHumansIn patientArterial PressureGlasgow Coma Scale0101 mathematicsAgedMonitoring PhysiologicSurvey Researchbusiness.industrylcsh:R010102 general mathematicsGlasgow Coma Scalemedicine.diseasePolymer ChemistryHealth CareMedical intensive care unitHealth Care FacilitiesPeople and Placeslcsh:QPopulation GroupingsbusinessSoftwarePLoS ONE
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Temporal weights in loudness: Investigation of the effects of background noise and sound level

2019

Previous research has consistently shown that for sounds varying in intensity over time, the beginning of the sound is of higher importance for the perception of loudness than later parts (primacy effect). However, in all previous studies, the target sounds were presented in quiet, and at a fixed average sound level. In the present study, temporal loudness weights for a time-varying narrowband noise were investigated in the presence of a continuous bandpass-filtered background noise and the average sound levels of the target stimuli were varied across a range of 60 dB. Pronounced primacy effects were observed in all conditions and there were no significant differences between the temporal w…

MaleTime FactorsVisionLoudness PerceptionInformation TheorySocial SciencesNervous SystemMathematical and Statistical TechniquesAnimal CellsMedicine and Health SciencesPsychologyNeuronsNervesPhysicsStatisticsQRAmbient NoiseSoundPhysical SciencesEngineering and TechnologyMedicineFemaleSound PressureSensory PerceptionAnatomyCellular TypesPerceptual Maskingpsychological phenomena and processesPsychoacousticsResearch ArticleAdultComputer and Information SciencesAdolescentScienceModels PsychologicalResearch and Analysis MethodsAuditory NervesYoung Adultotorhinolaryngologic diseasesHumansStatistical MethodsBackground Signal NoiseBiology and Life SciencesAuditory ThresholdAcousticsCell BiologyAcoustic StimulationCellular Neuroscience150 PsychologieSignal ProcessingNoise150 PsychologyMathematicsNeuroscienceForecasting
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RNAPol-ChIP: a novel application of chromatin immunoprecipitation to the analysis of real-time gene transcription.

2004

We describe a procedure, RNAPol-ChIP, to measure actual transcriptional rate. It consists of the detection, by chromatin immunoprecipitation (ChIP), of RNA polymerase II within the coding region of genes. To do this, the DNA immunoprecipitated with polymerase antibodies is analysed by PCR, using an amplicon well within the coding region of the desired genes to avoid interferences with polymerase paused at the promoter. To validate RNAPol-ChIP, we compare our results to those obtained by classical methods in several genes induced during either liver regeneration or acute pancreatitis. When short half-life mRNA genes are studied (e.g. c-fos and egr1), RNAPol-ChIP gives results similar to thos…

MaleTranscription GeneticRNA polymerase IIPolymerase Chain ReactionTranscription (biology)GeneticsCoding regionAnimalsRNA MessengerRats WistarGenePolymeraseNAR Methods OnlinebiologyGenes fosAmpliconMolecular biologyPrecipitin TestsChromatinCell biologyChromatinLiver RegenerationRatsKineticsLiverPancreatitisAcute Diseasebiology.proteinRNA Polymerase IIChromatin immunoprecipitationNucleic acids research
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Umbilical cord blood transplantation from unrelated donors in patients with Philadelphia chromosome-positive acute lymphoblastic leukemia

2014

Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't; There are very few disease-specific studies focusing on outcomes of umbilical cord blood transplantation for Philadelphia chromosome-positive acute lymphoblastic leukemia. We report the outcome of 45 patients with Philadelphia chromosome-positive acute lymphoblastic leukemia who underwent myeloablative single unit cord blood transplantation from unrelated donors within the GETH/GITMO cooperative group. Conditioning regimens were based on combinations of thiotepa, busulfan, cyclophospamide or fludarabine, and antithymocyte globulin. At the time of transplantation, 35 patients (78%) were in first complete remission, four (8%) …

MaleTransplantation Conditioning:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Case-Control Studies::Retrospective Studies [Medical Subject Headings]Leucemia-linfoma linfoblástico de células precursorasGraft vs Host Disease:Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings]Supervivencia sin EnfermedadGastroenterology:Anatomy::Body Regions::Transplants::Allografts [Medical Subject Headings]:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents::Myeloablative Agonists [Medical Subject Headings]:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]hemic and lymphatic diseasesMedicineCumulative incidenceTasa de SupervivenciaPhiladelphia ChromosomeChildCromosoma Filadelfia:Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Disease Attributes::Chronic Disease [Medical Subject Headings]:Named Groups::Persons::Age Groups::Child::Child Preschool [Medical Subject Headings]ArticlesHematologyMiddle AgedPrecursor Cell Lymphoblastic Leukemia-LymphomaAllografts:Analytical Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Biological Therapy::Immunomodulation::Immunotherapy::Immunosuppression::Transplantation Conditioning [Medical Subject Headings]FludarabineSurvival RateChild Preschool:Named Groups::Persons::Age Groups::Adolescent [Medical Subject Headings]:Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Chromosome Aberrations::Translocation Genetic::Philadelphia Chromosome [Medical Subject Headings]:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Survival Analysis::Disease-Free Survival [Medical Subject Headings]FemaleAcondicionamiento para el trasplanteCord Blood Stem Cell TransplantationTrasplante de células madre de la sangre del cordónmedicine.drugAloinjertosAdultmedicine.medical_specialtyAdolescent:Check Tags::Male [Medical Subject Headings]Cord Blood Stem Cell TransplantationPhiladelphia chromosomeEnfermedad injerto contra huéspedDisease-Free SurvivalEstudios retrospectivosInternal medicine:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Follow-Up Studies [Medical Subject Headings]:Named Groups::Persons::Age Groups::Adult [Medical Subject Headings]HumansPreschoolSurvival rate:Named Groups::Persons::Age Groups::Child [Medical Subject Headings]Retrospective Studies:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Vital Statistics::Mortality::Survival Rate [Medical Subject Headings]business.industryUmbilical Cord Blood TransplantationEnfermedad crónicaAgonistas mieloablativosMyeloablative Agonistsmedicine.diseaseSettore MED/15SurgeryTransplantation:Diseases::Immune System Diseases::Graft vs Host Disease [Medical Subject Headings]:Diseases::Hemic and Lymphatic Diseases::Lymphatic Diseases::Lymphoproliferative Disorders::Leukemia Lymphoid::Precursor Cell Lymphoblastic Leukemia-Lymphoma [Medical Subject Headings]:Check Tags::Female [Medical Subject Headings]Estudios de SeguimientoChronic Disease:Analytical Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures Operative::Transplantation::Cell Transplantation::Stem Cell Transplantation::Cord Blood Stem Cell Transplantation [Medical Subject Headings]Adolescent; Adult; Allografts; Child; Child Preschool; Chronic Disease; Disease-Free Survival; Female; Follow-Up Studies; Graft vs Host Disease; Humans; Male; Middle Aged; Myeloablative Agonists; Retrospective Studies; Survival Rate; Cord Blood Stem Cell Transplantation; Philadelphia Chromosome; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Transplantation ConditioningbusinessBusulfanFollow-Up Studies
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Glucocorticoid receptor knockdown decreases the antioxidant protection of B16 melanoma cells: an endocrine system-related mechanism that compromises …

2014

We previously reported an interorgan system in which stress-related hormones (corticosterone and noradrenaline), interleukin-6, and glutathione (GSH) coordinately regulate metastatic growth of highly aggressive B16-F10 melanoma cells. Corticosterone, at levels measured in tumor-bearing mice, also induces apoptotic cell death in metastatic cells with low GSH content. In the present study we explored the potential role of glucocorticoids in the regulation of metastatic cell death/survival during the early stages of organ invasion. Glucocorticoid receptor (GCR) knockdown decreased the expression and activity of γ-glutamylcysteine synthetase (γ-GCS), the rate-limiting step in GSH synthesis, in …

MaleTumor PhysiologyGlutathione reductaseCancer TreatmentMelanoma ExperimentalGene Expressionlcsh:MedicineBiochemistryAntioxidantsMetastasisAnalytical Chemistrychemistry.chemical_compoundOxidative DamageMiceGlucocorticoid receptorSpectrum Analysis TechniquesCell SignalingNeoplasmsMolecular Cell BiologyBasic Cancer ResearchMedicine and Health SciencesNeoplasm Metastasislcsh:Sciencechemistry.chemical_classificationMultidisciplinaryCell DeathGlutathione peroxidaseEndocrine TherapyFlow CytometryGlutathioneChemistrymedicine.anatomical_structureOncologyResearch DesignSpectrophotometryPhysical SciencesCytophotometryGlucocorticoidmedicine.drugResearch ArticleSignal Transductionmedicine.medical_specialtyEndotheliumClinical Research DesignCell SurvivalGlutamate-Cysteine LigaseDown-RegulationEndocrine SystemBiologyResearch and Analysis MethodsCell LineReceptors GlucocorticoidInternal medicineCell Line TumormedicineGeneticsAnimalsHumansAnimal Models of DiseaseOncogenic Signalinglcsh:RBiology and Life SciencesEndothelial CellsGlutathioneCell BiologyMice Inbred C57BLEndocrinologyHEK293 CellschemistryCell cultureCancer cellAnimal Studieslcsh:QEndothelium VascularCytometryPLoS ONE
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Cerebral haemodynamic changes during propofol-remifentanil or sevoflurane anaesthesia: transcranial Doppler study under bispectral index monitoring

2006

Background. Sevoflurane or propofol–remifentanil-based anaesthetic regimens represent modern techniques for neurosurgical anaesthesia. Nevertheless, there are potential differences related to their activity on the cerebrovascular system. The magnitude of such difference is not completely known. Methods. In total 40 patients, treated for spinal or maxillo-facial disorders, were randomly allocated to either i.v. propofol–remifentanil or inhalational sevoflurane anaesthesia. Transcranial Doppler was used to assess changes in cerebral blood flow velocity, carbon dioxide reactivity, cerebral autoregulation and the bispectral index to assess the depth of anaesthesia. Results. Time-averaged mean f…

MaleUltrasonography Doppler TranscranialHemodynamicsBlood PressurePiperidinesHeart RateMedicineHomeostasisProspective StudiesPropofolUltrasonographyIntraoperativeAdult Anesthetics; Combined; pharmacology Anesthetics; Inhalation; Intravenous; pharmacology Blood Flow Velocity; drug effects Blood Pressure; drug effects Carbon Dioxide; physiology Cerebrovascular Circulation; drug effects Electroencephalography Female Heart Rate; drug effects Homeostasis; drug effects Humans Male Methyl Ethers; pharmacology Middle Aged Monitoring; Intraoperative; methods Piperidines; pharmacology Propofol; pharmacology Prospective Studies Ultrasonography; Doppler; TranscranialCombineddrug effectDopplerElectroencephalographyMiddle AgedAnesthetics CombinedCerebral blood flowInhalationBispectral indexAnesthesiaCerebrovascular CirculationAnesthetics InhalationmethodFemaleIntravenousPropofolAnesthetics IntravenousBlood Flow Velocitymedicine.drugHumanMethyl EthersAdultMonitoringRemifentanilIntravenouTranscranialCerebral autoregulationtranscranial DopplerSevofluranemethodsRemifentanilSevofluranePiperidineMonitoring IntraoperativeHomeostasiHumansAnestheticsbusiness.industryAnestheticCarbon DioxideTranscranial DopplerProspective StudieAnesthesiology and Pain MedicineMethyl Etherdrug effectsphysiologypharmacologybusiness
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Cancer mortality inequalities in urban areas: a Bayesian small area analysis in Spanish cities

2011

incluye "Erratum to: Cancer mortality inequalities in urban areas: a Bayesian small area analysis in Spanish cities" BACKGROUND: Intra-urban inequalities in mortality have been infrequently analysed in European contexts. The aim of the present study was to analyse patterns of cancer mortality and their relationship with socioeconomic deprivation in small areas in 11 Spanish cities. METHODS: It is a cross-sectional ecological design using mortality data (years 1996-2003). Units of analysis were the census tracts. A deprivation index was calculated for each census tract. In order to control the variability in estimating the risk of dying we used Bayesian models. We present the RR of the censu…

MaleUrban PopulationEstudios transversalesCross-sectional studyEspaña:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies [Medical Subject Headings]Business Management and Accounting(all)Disparidades en el estado de saludPoblación urbanaHealth informatics:Health Care::Population Characteristics::Population::Urban Population [Medical Subject Headings]NeoplasmsHuman geographyEpidemiologyCàncerUrban areasSocioeconomicsSmall-Area Analysismedia_common:Geographicals::Geographic Locations::Europe::Spain [Medical Subject Headings]Geography:Diseases::Neoplasms [Medical Subject Headings]CensusNeoplasiasGeography:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Statistics as Topic::Probability::Bayes Theorem [Medical Subject Headings]lcsh:R858-859.7EnfermeríaFemaleRisk assessmentComputer Science(all)Riskmedicine.medical_specialtyGeneral Computer ScienceInequalitymedia_common.quotation_subjectHealth geographyeducationBayesian probabilityMedi ambientCancer mortalitylcsh:Computer applications to medicine. Medical informaticsRisk AssessmentCàncer -- MortalitatCiutatsMortalitatmedicineConfidence IntervalsTeorema de BayesHumansCancer -- MortalitySocioeconomic statusPovertyPovertybusiness.industryPublic healthResearchPublic Health Environmental and Occupational HealthCorrection:Health Care::Environment and Public Health::Public Health::Epidemiologic Measurements::Demography::Health Status::Health Status Disparities [Medical Subject Headings]Bayes TheoremHealth Status DisparitiesGeneral Business Management and AccountingSocioeconomic deprivationBayesian statistical decisionCross-Sectional StudiesEstadística bayesianaSocioeconomic FactorsSpainInequalitiesbusinessDemographyInternational Journal of Health Geographics
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