Search results for "methods"
showing 10 items of 4526 documents
A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences.
2020
To facilitate preclinical testing of T-cell receptors (TCRs) derived from tumor-reactive T-cell clones it is necessary to develop convenient and rapid cloning strategies for the generation of TCR expression constructs. Herein, we describe a pDONR™221 vector backbone allowing to generate Gateway™ compatible entry clones encoding optimized bicistronic αβTCR constructs. It harbors P2A-linked TCR constant regions and head-to-head-oriented recognition sites of the Type IIS restriction enzymes BsmBI and BsaI for seamless cloning of the TCRα and TCRβ V(D)J regions, respectively. Additional well-established TCR optimizations were incorporated to enhance TCR functionality. This included replacing of…
Influence of electronic and geometric properties on melting of sodium clusters
2004
Systematics of the melting transition for sodium clusters with 40-355 atoms has been studied with both ab initio and semiclassical molecular dynamics simulations. The melting temperatures obtained with an ab initio method for Na55 + and Na93 + correlate well with the experimental results. The semiclassically determined melting temperatures show similarities with the experimentally determined ones in the size region from 55 to 93 and near size 142, and the latent heat in the size region from 55 to 139, but not elsewhere in the size region studied. This indicates that the nonmonotonical melting behavior observed experimentally cannot be fully explained by geometrical effects. The semiclassica…
Energy-Stable Numerical Schemes for Multiscale Simulations of Polymer–Solvent Mixtures
2017
We present a new second-order energy dissipative numerical scheme to treat macroscopic equations aiming at the modeling of the dynamics of complex polymer–solvent mixtures. These partial differential equations are the Cahn-Hilliard equation for diffuse interface phase fields and the Oldroyd-B equations for the hydrodynamics of the polymeric mixture. A second-order combined finite volume/finite difference method is applied for the spatial discretization. A complementary approach to study the same physical system is realized by simulations of a microscopic model based on a hybrid Lattice Boltzmann/Molecular Dynamics scheme. These latter simulations provide initial conditions for the numerical…
Novel second-order nonlinear optical polymer materials containing indandione derivativatives as a chromophore
2006
Second order non linear optical (NLO) properties of sPMMA based host-guest systems containing eight dimethylaminobenzylidene -1, 3 - indandione (DMABI) related chromophores have been investigated by means of quantum chemical calculations and SHG experimental characterization. Ab initio calculations with basis set 6-31G were used for molecular geometry determination as well as in the calculations of molecular hyperpolarizability by FF approach. Influence of the chromophore concentration on the host-guest film NLO performance was obtained by SHG Maker fringe experiments. The highest value of d53233 =80 pm/V (frequency corrected value d033 =12 pm/V) at chromophore concentration 15 %wt have bee…
Morphometry of Middle Bronze Age palstaves by discrete cosine transform.
2009
9 pages; International audience; The Discrete Cosine Transform (DCT) is a Fourier-related transform widely used in signal processing and well suited to the analysis of open outlines. This method was applied here to evaluate the discrimination power of the inner lateral rib for two palstave populations dating from the Middle Bronze Age, excavated in northwest France. A corpus of almost 400 palstaves (bronze axes) of the Breton and Norman types was processed, and compared to specimens found at Sermizelles in Burgundy. The procedure is robust and produces a discrimination in good agreement with the traditional typology. Besides the definition of a ‘standard' shape for each population, the morp…
Inland harbours in Central Europe:: Junctions between Northern Europe and the Mediterranean Sea
2016
The international symposium organized in Dijon offers a structural and diachronic comparative analysis of the harbours networks on inland waterbodies. Its goals are the study of the harbours settled on lakes or rivers as a complex phenomenon, and the assessment of their respective significance as hubs in the communication and exchange network between Mediterranean Sea and Northern Europe.These two days gather German, French and European researchers from the different fields required on such a research problem (history, archaeology,geography, palaeoenvironmental analysis, geomatics), in the aim to present recent discoveries and results.The present book contains the 27 abstracts in three lang…
Speech-input multi-target machine translation
2007
In order to simultaneously translate speech into multiple languages an extension of stochastic finite-state transducers is proposed. In this approach the speech translation model consists of a single network where acoustic models (in the input) and the multilingual model (in the output) are embedded. The multi-target model has been evaluated in a practical situation, and the results have been compared with those obtained using several mono-target models. Experimental results show that the multi-target one requires less amount of memory. In addition, a single decoding is enough to get the speech translated into multiple languages.
Studying Nucleosomes Positioning by a Multi-Layer Model
2007
Eukaryotic DNA is packaged into a highly compact and dynamic structure called chromatin. While this packaging allows the cell to organize a large and complex genome in the nucleus, it can also block the access of transcription factors and other proteins to DNA. Nucleosomes are the fundamental repeating units of eukaryotic chromatin. Nucleosome position can be regulated in vivo by multi-subunit chromatin remodeling complexes, and their position can influence gene expression in eukaryotic cells. Alterations in chromatin structure, and hence in nucleosome organization, can result in a variety of diseases, including cancer, highlighting the need to achieve a better understanding of the molecula…
Indicadores compuestos como metodología innovadora en Comunicación. Aplicación para la evaluación de los medios públicos europeos
2020
The currently dominant scientific production model in the field of Communication exhibits an excess of standardization towards mainly empirical work that is overwhelming caried out using quantitative techniques based on simple statistical analyses, in many cases limiting the presentation of results to a reading of the available data. This article presents an innovative proposal in this area, designed as a new form of dissemination and scientific presentation, with the objective of constructing three composite indicators that, when applied to European public service media, will enable the assessment of the perception of their social value, independence, and management efficiency. Another aim…
Dimension enrichment with factual data during the design of multidimensional models: application to bird biodiversity
2015
20 pages; International audience; Data warehouses (DW) and OLAP systems are technologies allowing the on-line analysis of huge volume of data according to decision-makers’ needs. Designing DW involves taking into account functional requirements and data sources (mixed design methodology) [1]. But, for complex applications, existing automatic design methodologies seem inefficient. In some cases, decision-makers need querying, as a dimension, data which have been defined as facts by actual automatic mixed approachs. Therefore, in this paper, we offer a new mixed refinement methodology relevant to constellation multidimensional schema. The proposed methodolgy allows to decision-makers to enric…