Search results for "methods"
showing 10 items of 4526 documents
A maChine and deep Learning Approach to predict pulmoNary hyperteNsIon in newbornS with congenital diaphragmatic Hernia (CLANNISH): Protocol for a re…
2021
Introduction Outcome predictions of patients with congenital diaphragmatic hernia (CDH) still have some limitations in the prenatal estimate of postnatal pulmonary hypertension (PH). We propose applying Machine Learning (ML), and Deep Learning (DL) approaches to fetuses and newborns with CDH to develop forecasting models in prenatal epoch, based on the integrated analysis of clinical data, to provide neonatal PH as the first outcome and, possibly: favorable response to fetal endoscopic tracheal occlusion (FETO), need for Extracorporeal Membrane Oxygenation (ECMO), survival to ECMO, and death. Moreover, we plan to produce a (semi)automatic fetus lung segmentation system in Magnetic Resonanc…
Diagnostics of autoimmune bullous diseases in German dermatology departments
2012
Summary Background: No consistent data are available on the currently employed diagnostic tools for autoimmune bullous diseases in Germany. The aim of this survey was to describe currently performed diagnostic methods for bullous autoimmune diseases in German dermatology departments. Methods: A standardized questionnaire evaluated the available diagnostic methods i. e. direct immunofluorescence microscopy (IFM), indirect IFM, commercial ELISA systems, and non-commercial serological tests as well as the number of samples per year in all 34 university and 39 non-university dermatology departments. Results: The overall return rate was 89 %, 100 % and 79 % for the university and non-university …
From Pentalene to Dicyclopenta[b,g]naphthalene, or the Change towards Delocalized Structures
2005
Lining triples-corrected coupled-cluster methods as well as other high-level theoretical approximations, the optimized parameters and isomerization barriers of the family of compounds cyclopentadiene-(benzene) x -cyclopentadiene (x 0, 1, 2) are computed. In contrast to previous studies, s-indacene presents a localized C 2 h geometry. Also, the localized structure of pentalene is found to be the most stable, but when two benzene rings are intercalated between the five-member rings of pentalene, the resulting molecule preferably adopts a delocalized D 2 h conformation.
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
2009
We describe a quantitative method for detecting RNA alternative splicing variants that combines in situ hybridization of fluorescently labeled peptide nucleic acid (PNA) probes with confocal microscopy Förster resonance energy transfer (FRET). The use of PNA probes complementary to sequences flanking a given splice junction allows to specifically quantify, within the cell, the RNA isoform generating such splice junction by FRET measure. As a proof of concept we analyzed two alternative splicing events originating from lymphocyte antigen 6 (LY6) complex, locus G5B (LY6G5B) pre-mRNA. These are characterized by the removal of the first intron (Fully Spliced Isoform, FSI) or by retention of suc…
Adaptation and Psychometric Properties of the Scale of Positive and Negative Experience (SPANE) in the General Colombian Population
2021
(1) Background: Diener’s Scale of Positive and Negative Experiences (SPANE) assesses the presence and intensity of positive and negative affects, since these are considered basic aspects of the study of well-being. This article studies its psychometric properties in the general Colombian population. (2) We conducted a cross-sectional study of a sample of 1255 Colombians and we used structural equation modeling to confirm the bifactor structure. Additionally, we studied invariance by gender, and convergent and concurrent validity. (3) We found acceptable fit indicators for the bifactor model (CFI = 0.889, RMSEA = 0.046, SRMR = 0.059) as well as for the convergent (CFI = 0.909, RMSEA = 0.050,…
Decoding Emotional Valence from Electroencephalographic Rhythmic Activity
2017
We attempt to decode emotional valence from electroencephalographic rhythmic activity in a naturalistic setting. We employ a data-driven method developed in a previous study, Spectral Linear Discriminant Analysis, to discover the relationships between the classification task and independent neuronal sources, optimally utilizing multiple frequency bands. A detailed investigation of the classifier provides insight into the neuronal sources related with emotional valence, and the individual differences of the subjects in processing emotions. Our findings show: (1) sources whose locations are similar across subjects are consistently involved in emotional responses, with the involvement of parie…
Ab-initio Calculation of the Properties of Permalloys Used in Electrical Machines
2006
In this paper an ab-initio calculation that allows to describe the main features of permalloys is presented. The calculation is bawd on Korringa-Kohn-Rostoker electronic structure method. The dependence of the magnetocryttalline anisotropy and of the magnetostriction on the concentration of non magnetic addiction is computed.
Draft Genome Sequences of Dickeya sp. Isolates B16 (NIB Z 2098) and S1 (NIB Z 2099) Causing Soft Rot of Phalaenopsis Orchids
2015
ABSTRACT The genus Dickeya contains bacteria causing soft rot of economically important crops and ornamental plants. Here, we report the draft genome sequences of two Dickeya sp. isolates from rotted leaves of Phalaenopsis orchids.
Citrus aurantium L. Active Constituents, Biological Effects and Extraction Methods. An Updated Review
2021
Citrus genus is a prominent staple crop globally. Long-term breeding and much hybridization engendered a myriad of species, each characterized by a specific metabolism generating different secondary metabolites. Citrus aurantium L., commonly recognized as sour or bitter orange, can exceptionally be distinguished from other Citrus species by unique characteristics. It is a fruit with distinctive flavor, rich in nutrients and phytochemicals which possess different health benefits. This paper presents an overview of the most recent studies done on the matter. It intends to provide an in-depth understanding of the biological activities and medicinal uses of active constituents existing in C. au…
Procedures for Antibiotic Residues in Bovine Muscle Tissues
2011
Abstract Acetonitrile extraction followed by primary-secondary amine dispersive SPE cleanup QuEChERS (quick, easy, cheap, effective, rugged, and safe), was compared to pressurized liquid extraction (PLE) using water at 70°C for 10 min at 1500 psi for the determination of 16 veterinary drugs in bovine muscle tissues by LC/MS/MS. PLE was significantly more effective for the extraction of veterinary drugs (ranging from 69 to 103% with RSD ≤ 18%) than QuEChERS (ranging from 19 to 89% with RSD ≤ 19%). Linearity of the calibration curves was obtained over the range considered (from 10 μg/kg or LOQ to 1000, μg/kg) with r2 ≥ 0.99 for all the analytes by both methods. Although an internal standard w…