Search results for "methods"
showing 10 items of 4526 documents
Translational Approaches In Cardiovascular Diseases by Omics.
2017
Cardiovascular diseases are among the leading causes of morbidity and mortality. Despite scientific and technical progress in risk prediction, diagnostics, prognostication and therapy of cardiovascular pathologies, new biomarkers and therapeutic targets remain the subject of intense research to reduce the burden of these diseases. High throughput analyses, termed "omics", are a promising avenue of research. These recently developed technical fields have revolutionized biological and medical research in a very short time. By their interdisciplinary nature, these new methods have already provided a wide vision of cell and tissue pathways and functions. Here, we review how these methods can he…
CiliaCarta: An integrated and validated compendium of ciliary genes
2019
The cilium is an essential organelle at the surface of mammalian cells whose dysfunction causes a wide range of genetic diseases collectively called ciliopathies. The current rate at which new ciliopathy genes are identified suggests that many ciliary components remain undiscovered. We generated and rigorously analyzed genomic, proteomic, transcriptomic and evolutionary data and systematically integrated these using Bayesian statistics into a predictive score for ciliary function. This resulted in 285 candidate ciliary genes. We generated independent experimental evidence of ciliary associations for 24 out of 36 analyzed candidate proteins using multiple cell and animal model systems (mouse…
Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis
2022
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Review of current and “omics” methods for assessing the toxicity (genotoxicity, teratogenicity and nephrotoxicity) of herbal medicines and mushrooms
2012
Ethnopharmacological relevance: The increasing use of traditional herbal medicines around the world requires more scientific evidence for their putative harmlessness. To this end, a plethora of methods exist, more or less satisfying. In this post-genome era, recent reviews are however scarce, not only on the use of new "omics" methods (transcriptomics, proteomics, metabonomics) for genotoxicity, teratogenicity, and nephrotoxicity assessment, but also on conventional ones. Methods: The present work aims (i) to review conventional methods used to assess genotoxicity, teratogenicity and nephrotoxicity of medicinal plants and mushrooms; (ii) to report recent progress in the use of "omics" techn…
High-accuracy extrapolated ab initio thermochemistry. II. Minor improvements to the protocol and a vital simplification
2006
The recently developed high-accuracy extrapolated ab initio thermochemistry method for theoretical thermochemistry, which is intimately related to other high-precision protocols such as the Weizmann-3 and focal-point approaches, is revisited. Some minor improvements in theoretical rigor are introduced which do not lead to any significant additional computational overhead, but are shown to have a negligible overall effect on the accuracy. In addition, the method is extended to completely treat electron correlation effects up to pentuple excitations. The use of an approximate treatment of quadruple and pentuple excitations is suggested; the former as a pragmatic approximation for standard cas…
Current density maps, magnetizability, and nuclear magnetic shielding tensors of bis-heteropentalenes. II. Furo-furan Isomers
2004
Magnetic susceptibility and nuclear magnetic shielding at the nuclei of bis-heteropentalenes formed by two furan units ([2,3-b], [3,2-b], [3,4-b], and [3,4-c] isomers) have been computed by several approximated techniques and a large Gaussian basis set to achieve near Hartree–Fock estimates. Ab initio models of the ring currents induced by a magnetic field normal to the molecular plane were obtained for the three isomeric systems of higher symmetry, showing that the π electrons give rise to intense diamagnetic circulation. The π currents are responsible for enhanced magnetic anisotropy and strong out-of-plane proton deshielding. The theoretical findings are used to build up a “diatropicity …
Influence of structure on the polarizability of hydrated methane sulfonic acid clusters
2015
Abstract: The relationship between polarizability and structure is investigated in methane sulfonic acid (MSA) and in 36 hydrated MSA clusters. The polarizabilities are calculated at B3LYP and MP2 level and further partitioned into molecular contributions using classic and iterative Hirshfeld methods. The differences in the two approaches for partitioning of polarizabilities are thoroughly analyzed. The polarizabilities of the molecules are found to be influenced in a systematic way by the hydrogen bond network in the clusters, proton transfer between MSA and water molecules, and weak interactions between water molecules and the methyl group of MSA.
Reinterpretation of Classic Proton Charge Form Factor Measurements
2020
In 1963, a proton radius of $0.805(11)~\mathrm{fm}$ was extracted from electron scattering data and this classic value has been used in the standard dipole parameterization of the form factor. In trying to reproduce this classic result, we discovered that there was a sign error in the original analysis and that the authors should have found a value of $0.851(19)~\mathrm{fm}$. We additionally made use of modern computing power to find a robust function for extracting the radius using this 1963 data's spacing and uncertainty. This optimal function, the Pad\'{e} $(0,1)$ approximant, also gives a result which is consistent with the modern high precision proton radius extractions.
About the finite convergence of the proximal point algorithm
1988
We study the finite convergence property of the proximal point algorithm applied to the partial inverse, with respect to a subspace, of the subdifferential of a polyhedral convex function. Using examples we show how sufficient conditions providing the finite convergence can be realized and we give a case with non finite termination.
Volviendo a pensar la alfabetización a través de los métodos mixtos: un estudio desde la mirada de los alumnos, las familias y el profesorado en la E…
2020
Los métodos mixtos presentan una alternativa sobre la investigación de la alfabetización como un fenómeno complejo. Este artículo analiza la integración de enfoques cuantitativos y cualitativos para hacer inferencias causales entre la alfabetización como práctica social y los diferentes espacios de interacción. Este estudio se realizó siguiendo un diseño secuencial de métodos mixtos. La muestra estuvo compuesta por 1.540 niños, 1.438 familias y 74 maestros. El proceso de recogida de información combinó cuestionarios y procedimientos etnográficos. Los datos se analizaron mediante la realización de análisis correlacionales, modelo de ecuaciones estructurales para grupos múltiples y análisis c…