Search results for "miR"
showing 10 items of 1117 documents
Checklist of the vascular plants collected during the 12th “Iter Mediterraneum” in Tunisia, 24 March – 4 April 2014
2015
The vascular plants (plus one alga) collected during Iter Mediterraneum XII of OPTIMA in North Tunisia have been studied. In all, 1374 gatherings were made from 43 localities in the “Cap Bon” region, “Dorsale Tunisienne”, “Tunisie du Nord-Est”, “Vallée de la Medjedah”, “Mogods” and “Kroumirie” areas. They belong to 82 families and 539 species or subspecies. Three taxa are new for the flora of Tunisia (Cerastium diffusum subsp. gussonei, Senecio leucanthemifolius subsp. mauritanicus, and Ulmus minor subsp. canescens), 7 represent new records for the “Kroumirie”, 15 for the “Mogods”, 3 for the “Tunisie du Nord-Est”, 2 for the “Dorsale Tunisienne”, and 8 for “Cap Bon”
The effects of protection measures on fish assemblage in the Plemmirio marine reserve (Central Mediterranean Sea, Italy): A first assessment 5years a…
2013
This 2-year study was aimed to investigate the early effects of protection measures on fish assemblage in the Plemmirio marine reserve and to evaluate its level of enforcement. Sampling was carried out by means of underwater visual census techniques in four sampling sites within the reserve boundaries and eight outside the reserve. Results showed significant inside/outside differences in the multivariate abundance of fish assemblage. These results were confirmed and exemplified by significant univariate differences between locations for total abundance, Species Richness and diversity of the fish assemblage; values of these metrics were higher inside the reserve than outside. Small fish size…
Role of sparc and MIR-29B1 in molecular effects induced by win in osteosarcoma MG63 cells
2014
SPARC (Secreted protein acidic and rich in cysteine) is considered as a prototype of matricellular protein due to its structure and the function that it displays in regulating cell/extracellular microenvironment interactions during development and in response to injury. Earlier studies underlined pleiotropic effects of intracellular SPARC on cancer growth and, in some cancer cell lines, identified it as a tumor suppressor protein. Objective This study aimed to evaluate the role of SPARC and its related miRNA in the molecular effects induced by the cannabinoid WIN in osteosarcoma MG63 cells. In these cells WIN is not able to induce cell death but sensitizes cells to TRAIL-mediated apoptotic …
UPREGULATION OF MIR-29A AND GENOMIC DNA HYPERMETHYLATION IN NORMAL KARYOTYPE AML SHOWING DNMT3A MUTATION UPREGOLAZIONE DEL MIR-29A E IPERMETILAZIONE …
2015
DNMT3A, a member of DNA methyltransferases, is mutated in approximately 22% of de novo normal karyotype acute myeloid leukemia (NK-AML) patients leading to adverse overall survival. The highly recurrent mutation in DNMT3A is a “gain of function-like” at codon R882. To indagate about miRNA signature in NK-AML R882-DNMT3A mutated we studied by qRT-PCR the expression of 384 known human miRNA in 9 selected de-novo AML DNMT3A mutated. We compared miRNA expression data with our previous results obtained in 31 AML DNMT3A wild type (WT) and we focused on a strong up-regulation of miR155, miR29a, miR196b and miR25. We consolidated this data in additional 24 new DNMT3A mutated AML and we confirmed th…
Relazione tra l’espressione del repressore trascrizionale MBP-1 e di miRNAs nel carcinoma mammario
2013
MBP-1 (Myc-promoter-binding-protein-1) è una proteina di 37 kDa, prodotta dalla traduzione alternativa del mRNA del gene ENO1, codificante per l’enzima glicolitico α-enolasi. MBP-1 svolge il ruolo di repressore trascrizionale agendo direttamente sul promotore di c-MYC, ERBB2 e COX2, tutti geni coinvolti in diverse fasi della progressione tumorale (1-3). Nei carcinomi duttali infiltranti della mammella (IDC), l’espressione di MBP-1 è inversamente correlata alla comparsa di metastasi linfonodali e di recidive (4). In diverse linee cellulari tumorali l’overespressione di MBP-1 risulta nella riduzione della proliferazione cellulare e dell’invasività e in un aumento nella morte cellulare per apo…
Upregulation of miR-29a and genomic DNA hypermethylation in normal karyotype AML showing DNMT3A mutation
2015
Acute Myeloid Leukaemia (AML) is frequently associated to normal karyotype and DNMT3A mutations (R882). Since we previously demonstrated distinctive miRNA expression in some AML groups, we study 384 miRNA in 9 selected DNMT3A-mutated NK-AML patients. Comparing these data with our previous results obtained in 31 DNMT3A-unmutated AML, we focused on a significant up-regulation of miR-155, miR-29a, miR-196b and miR-25. We investigated expression of these miRNAs in additional 24 DNMT3A-mutated AML patients and we confirm the up-regulation of miR-155, miR-29a and miR-196b; in particular, we judged very interesting the over expression of miR-29a since is known to directly target DNMT3A, TET1 and T…
Detecting significant features in modeling microRNA-target interactions
2017
MicroRNAs (miRNAs) are small non-coding RNA molecules mediating the translational repression and degradation of target mRNAs in the cell. Mature miRNAs are used as a template by the RNA-induced silencing complex (RISC) to recognize the complementary mRNAs to be regulated. Up to 60% of human genes are putative targets of one or more miRNAs. Several prediction tools are available to suggest putative miRNA targets, however, only a small part of the interaction pairs has been validated by experimental approaches. The analysis of the expression profile of the RNA fraction immunoprecipitated (IP) with the RISC proteins is an established method to detect which genes are actually regulated by the R…
MULTIOMIC ANALYSIS OF TRANSCRIPTIONAL REPRESSOR MBP-1 FUNCTIONS IN GYNECOLOGICAL TUMORS
2014
Dal trascrittoma all’interattoma di miRNA: identificazione sperimentale e bioinformatica delle interazioni funzionali miRNA:mRNA
2013
I miRNA, piccole molecole endogene di RNA non codificante, regolano l’espressione genica attraverso la degradazione dei messaggeri (mRNA) o l’inibizione della traduzione. I miRNA maturi interagiscono con le proteine del complesso RISC (RNA-induced silencing complex) tra cui le proteine Argonaute (Ago), capaci di legare direttamente i miRNA e di mediare la regolazione dell’espressione genica in seguito alla interazione del miRNA con il proprio mRNA target. Un singolo miRNA può legare diversi mRNA e ciascun mRNA può essere regolato da diversi miRNA. La maggior parte dei software di predizione oggi disponibili individuano i putativi target di singoli miRNA ignorando caratteristiche di tipo glo…