Search results for "microRNA."
showing 10 items of 574 documents
Prognostic and Functional Significant of Heat Shock Proteins (HSPs) in Breast Cancer Unveiled by Multi-Omics Approaches
2021
Simple Summary In this study, we investigated the expression pattern and prognostic significance of the heat shock proteins (HSPs) family members in breast cancer (BC) by using several bioinformatics tools and proteomics investigations. Our results demonstrated that, collectively, HSPs were deregulated in BC, acting as both oncogene and onco-suppressor genes. In particular, two different HSP-clusters were significantly associated with a poor or good prognosis. Interestingly, the HSPs deregulation impacted gene expression and miRNAs regulation that, in turn, affected important biological pathways involved in cell cycle, DNA replication, and receptors-mediated signaling. Finally, the proteomi…
Effects of anti-miR-182 on TSP-1 expression in human colon cancer cells: there is a sense in antisense?
2013
Abstract: Objective: miRNAs are attractive molecules for cancer treatment, including colon rectal cancer (CRC). We investigate on the molecular mechanism by which miR-182 could regulate thrombospondin-1 (TSP-1) expression, a protein down-regulated in CRC and inversely correlated with tumor vascularity and metastasis. Background: MicroRNAs are small non-coding RNAs that regulate the expression of different genes, involved in cancer progression, angiogenesis and metastasis. miR-182, over-expressed in colorectal cancer (CRC), has like predictive target thrombospondin-1 (TSP-1), a protein inversely correlated with tumor vascularity and metastasis that results downregulated in different types of…
Interplay Between MicroRNAs and Oxidative Stress in Ovarian Conditions with a Focus on Ovarian Cancer and Endometriosis
2019
Ovarian cancer and endometriosis are two distinct gynaecological conditions that share many biological aspects incuding proliferation, invasion of surrounding tissue, inflammation, inhibition of apoptosis, deregulation of angiogenesis and the ability to spread at a distance. miRNAs are small non-coding RNAs (19–22 nt) that act as post-transcriptional modulators of gene expression and are involved in several of the aforementioned processes. In addition, a growing body of evidence supports the contribution of oxidative stress (OS) to these gynaecological diseases: increased peritoneal OS due to the decomposition of retrograde menstruation blood facilitates both endometriotic lesion developmen…
Identificación de los mecanismos epigenéticos relacionados con los fenómenos de inmunosupresión en sepsis y su uso como biomarcadores de pronóstico
2023
La sepsis es un síndrome complejo que provoca una respuesta inmunitaria exacerbada como resultado a una infección, lo que puede causar disfunción orgánica y, en última instancia, la muerte. La sepsis puede avanzar a shock séptico (SS), caracterizado por una disfunción orgánica aguda que puede provocar una insuficiencia circulatoria y metabólica grave, aumentando el riesgo de mortalidad. Los pacientes con sepsis o SS ingresados durante al menos 7 días en la Unidad de Cuidados Intensivos (UCI) que presentan disfunción orgánica y necesidad de ventilación mecánica se clasifican como enfermos críticos crónicos (ECC), los cuales, si están ingresados al menos 14 días y cumplen una serie de caracte…
Expresión diferencial de microRNAs en tumores de mujeres jóvenes con cáncer de mama: Cribado de dianas moleculares
2015
El cáncer de mama constituye el cáncer invasivo más frecuente en mujeres, afectando a una de cada 8 mujeres a lo largo de su vida, siendo, además, la primera causa de muerte derivada de cáncer invasivo en la población española (15,5%). Las mujeres jóvenes representan únicamente el 3% del total de casos diagnosticados; sin embargo, estas pacientes presentan tumores de mayor tamaño e índice de proliferación, estando sobrerepresentados subtipos tumorales más agresivos y de peor pronóstico. Los microRNAs (miRNAs) son pequeñas moléculas de RNA no codificante, cuyo papel en la célula es regular la expresión de genes, bloqueando el paso de RNA mensajero a proteína. Su desregulación es típica en nu…
MiR-33a Controls hMSCS Osteoblast Commitment Modulating the Yap/Taz Expression Through EGFR Signaling Regulation
2019
Mesenchymal stromal cells (hMSCs) display a pleiotropic function in bone regeneration. The signaling involved in osteoblast commitment is still not completely understood, and that determines the failure of current therapies being used. In our recent studies, we identified two miRNAs as regulators of hMSCs osteoblast differentiation driving hypoxia signaling and cytoskeletal reorganization. Other signalings involved in this process are epithelial to mesenchymal transition (EMT) and epidermal growth factor receptor (EGFR) signalings through the regulation of Yes-associated protein (YAP)/PDZ-binding motif (TAZ) expression. In the current study, we investigated the role of miR-33a family as a (…
Estradiol deficiency and skeletal muscle apoptosis: Possible contribution of microRNAs.
2020
Background Menopause leads to estradiol (E2) deficiency that is associated with decreases in muscle mass and strength. Here we studied the effect of E2 deficiency on miR-signaling that targets apoptotic pathways. Methods C57BL6 mice were divided into control (normal estrous cycle, n = 8), OVX (E2 deficiency, n = 7) and OVX + E2 groups (E2-pellet, n = 4). Six weeks following the OVX surgery, mice were sacrificed and RNA isolated from gastrocnemius muscles. miR-profiles were studied with Next-generation sequencing (NGS) and candidate miRs verified using qPCR. The target proteins of the miRs were found using in silico analysis and measured at mRNA (qPCR) and protein levels (Western blot). Resu…
Aging and microRNA messaging : associations with systemic estrogen levels and physical performance
2018
This thesis took an innovative approach, which involved methodological development, to investigate microRNAs as candidate molecules and as genome-wide regulators of age, menopausal status and physical condition in both sexes. The focus was on potential serum microRNAs indicative of age, pre- or postmenopausal status, with or without estrogen- based hormone replacement therapy (HRT), and long-term physical training. MicroRNA associations with adiposity and inflammatory markers were also analyzed. Three different study designs were used: 1) the SAWEs study comprised healthy postmenopausal monozygotic twin sisters (n=11, 54-62 years) with discordance for HRT and premenopausal women (n=30, 20-4…
Impact of the flame retardant 2,2'4,4'-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles
2023
2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) is one of the most widespread environmental brominated flame-retardant congeners which has also been detected in animal and human tissues. Several studies have reported the effects of PBDEs on different health issues, including neurobehavioral and developmental disorders, reproductive health, and alterations of thyroid function. Much less is known about its immunotoxicity. The aim of our study was to investigate the effects that treatment of THP-1 macrophage-like cells with PBDE-47 could have on the content of small extracellular vesicles’ (sEVs) microRNA (miRNA) cargo and their downstream effects on bystander macrophages. To achieve this, we puri…
Correlación entre la expresión de microRNAs en hipertensión ocular versus glaucoma primario de ángulo abierto
2018
El Glaucoma Primario de Ángulo Abierto (GPAA), la primera causa mundial de ceguera irreversible, aumenta su prevalencia en mayores de 40 años. El primer factor de riesgo es la hipertensión ocular (HTO). No existe un biomarcador que pueda identificar sujetos con mayor predisposición hacia el GPAA. Planteamos la búsqueda de biomarcadores moleculares que ayuden a predecir el riesgo de padecer la enfermedad o de progresión de la misma. Las pequeñas moléculas de ARN no codificante (21-30 nucleótidos) que regulan la expresión génica a nivel posttranscripcional, se denominan miRNAs y se han relacionado con diversas patologías oculares. Esta Tesis Doctoral propone relacionar el perfil diferencial d…